BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0053.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC t... 773 0.0 gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC t... 318 4e-88 gi|300310681|ref|YP_003774773.1| amino acid ABC transporter peri... 283 2e-77 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 77 2e-15 gi|300312847|ref|YP_003776939.1| branched-chain amino acid ABC t... 66 6e-12 >gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 773 bits (1995), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/381 (100%), Positives = 381/381 (100%) Query: 1 MKTKLAIKAGVLAAMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEK 60 MKTKLAIKAGVLAAMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEK Sbjct: 1 MKTKLAIKAGVLAAMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEK 60 Query: 61 VKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPI 120 VKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPI Sbjct: 61 VKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPI 120 Query: 121 ETAEGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAA 180 ETAEGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAA Sbjct: 121 ETAEGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAA 180 Query: 181 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGYKGKIFQT 240 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGYKGKIFQT Sbjct: 181 GMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGYKGKIFQT 240 Query: 241 HSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQFGAGSRNL 300 HSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQFGAGSRNL Sbjct: 241 HSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQFGAGSRNL 300 Query: 301 FAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNYSPKDHFG 360 FAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNYSPKDHFG Sbjct: 301 FAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNYSPKDHFG 360 Query: 361 LGDDARIMLTIQNGNWKAVNP 381 LGDDARIMLTIQNGNWKAVNP Sbjct: 361 LGDDARIMLTIQNGNWKAVNP 381 >gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 318 bits (816), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 155/359 (43%), Positives = 235/359 (65%), Gaps = 4/359 (1%) Query: 23 HAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEKVKMIILDDATDPTQASKNARRL 82 A++ +GV +S TGP + LGIP KN AL P TIAG KV IILDDA+D TQA KNARR Sbjct: 20 RADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNYIILDDASDTTQAVKNARRF 79 Query: 83 VAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISP----IETAEGKDAWTFRLPQSTA 138 V+E+ VD+I+GS+ P ++++ +VA + QT ++++ +E + K W F+ PQ+ Sbjct: 80 VSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARIVEPMDAKKKWVFKTPQNDI 139 Query: 139 VMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAAGMKMGTVERFSRADTSVT 198 +M+ + M G + I F+GYS++YGE W +E ++ A +++ ERF+ D+S T Sbjct: 140 MMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGLKKLELVANERFAPTDSSTT 199 Query: 199 AQALRVVSSNPDAVLVVASGSGAAMPHKALIERGYKGKIFQTHSAASRDLIRLGGKDVEG 258 AQAL++V++ PDA+L+ ASG+ A +P +AL ERG+KG+I+QTH A+ D +R+GGK ++G Sbjct: 200 AQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQTHGVANADFLRVGGKMLDG 259 Query: 259 AYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQFGAGSRNLFAAHTYDAQLVLQKVVPE 318 + +GP +V LP SN +K+A+D+ T+YE G S +LF + +DA ++L+ +P Sbjct: 260 TLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVSLFGGYAWDAGVLLRWAIPV 319 Query: 319 ALKKAKPGTPAFRAALKEALETSGPIQITQGTLNYSPKDHFGLGDDARIMLTIQNGNWK 377 ALK AKPGTP FRAAL++ LE + + G S DH GL A +M+ I +G W+ Sbjct: 320 ALKGAKPGTPEFRAALRDVLEATKEVTGVHGVYTMSSTDHLGLDQRAAVMVKIVDGAWQ 378 >gi|300310681|ref|YP_003774773.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 283 bits (724), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 161/383 (42%), Positives = 226/383 (59%), Gaps = 10/383 (2%) Query: 1 MKTKLAIKAGVLAAMLACA-GIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGE 59 M K I A VL M + A A A++ +GVV S TGP S +GIP KN L P I Sbjct: 1 MLHKRLIAAVVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 Query: 60 KVKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISP 119 KV+ I LDDA+DPT++ ++L+AE VD IIG + P + + E LA P Sbjct: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLA--P 118 Query: 120 IETA------EGKDAWTFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEV 173 + TA + W F+ Q+ ++A +V+QM K VKT GF+ +D +GENW + Sbjct: 119 VGTAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAGFIAVNDPFGENWARVF 178 Query: 174 QRLATAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGY 233 LA +K+ ER+ R DTSVTAQ+L++V++NPDAVLV A G+ +P L ++GY Sbjct: 179 SDLAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGY 238 Query: 234 KGKIFQTHSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQF 293 KGKI+QTH AA ++LG VEG + + +V +PDSN SKK+A D++ Y+K + Sbjct: 239 KGKIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVATDYIEAYKKMY 298 Query: 294 GAGSRNLFAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNY 353 A F A+ YDA L+L+K +P A +KAKPG+ FRAAL++ALE + + TQG N Sbjct: 299 NAVPAT-FGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNM 357 Query: 354 SPKDHFGLGDDARIMLTIQNGNW 376 S DH G D R ++T++ GNW Sbjct: 358 SATDHSGFDDRGRELITVKGGNW 380 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 89/332 (26%), Positives = 147/332 (44%), Gaps = 33/332 (9%) Query: 7 IKAGVLAAMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETI------AGEK 60 I A ++ A L+ A +A A++ +GV +LTGP + G+ KNGF L E I G+K Sbjct: 10 IPALLIGATLSQAALA-ADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDK 68 Query: 61 VKMIILDDATDPTQASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPI 120 + ++I D+ +A ++L+ +DKV + G A +A + V S Sbjct: 69 LALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTS-- 126 Query: 121 ETAEGKDA---WTFRLPQSTA-VMAGGVVNQMKKLGVKTIGFL-GYSDSYGENWLKEVQR 175 TA+G A +TFR A V+ V +K G+K + + G D++ ++ + Sbjct: 127 NTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKA 186 Query: 176 LATAAGMKMGTVERFSRADTSVTAQALRVVSSNPDAVLVVASGSGAA---MPHKALIERG 232 + + T E +++ D AQ ++ + NPDA++ AA + +AL G Sbjct: 187 TLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRAL---G 243 Query: 233 YKGKIFQTHSAASRDLIRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQ 292 K + S L + KD ++ P P + A F+T Y+ + Sbjct: 244 MKQPFIGGNGLNSPKLFEI-AKDAGDNTLMGSPWSAENQTPANKA-------FITAYKAK 295 Query: 293 FGAGSRNLFAAHTYDAQLVLQKVVPEALKKAK 324 FGA + FAA YDA +V +ALK K Sbjct: 296 FGA-DPDQFAAQAYDAMY----IVADALKNVK 322 >gi|300312847|ref|YP_003776939.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 65.9 bits (159), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 89/363 (24%), Positives = 147/363 (40%), Gaps = 47/363 (12%) Query: 20 GIAHAELTIGVVMSLTGPGSGLG----IPAKNGFALWPET--IAGEKVKMIILDDATDPT 73 G++ E+ +G +GP S LG + A++ FA+ + G +V+++ LDD +P Sbjct: 29 GVSATEVVLGQSAPFSGPASDLGRDFLVGAQSYFAMVNAKGGVHGRRVRLLSLDDGYEPE 88 Query: 74 QASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISPIETA----EGKDAW 129 + + N ++L+ DKV + G + TLA A A+ V L +P+ A E + Sbjct: 89 RTAANTQQLIWRDKVFALFGYSGTATTLA-ALPAIREANVPL-FAPVSGALALREPFNRL 146 Query: 130 TFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAAGMKMGTVER 189 F + S A ++ Q++ G + + +D G++ L++++ A G+ + Sbjct: 147 IFHVRASYAQETDFLLRQLQGTGRRKVAVFYQNDELGKSMLEQLRLRALVYGLTLVGTAA 206 Query: 190 FSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIER----GYKGKIFQTHSAAS 245 R +V A ++ PD V+ V S P ALI R GY G S Sbjct: 207 VERNSDAVEGAAQALLKVKPDVVIQVVS----YRPAAALIARMRQAGYLGGFSHYSFVGS 262 Query: 246 RDL---IRLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEK---QFGAGSRN 299 L ++ G E A V+ P K + V Y K Q N Sbjct: 263 LSLSARLKECGIGAEIAQVVPFP-------------NKARLPLVYEYRKAVAQHPGAVDN 309 Query: 300 LFAAHTYDAQLVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTL--NYSPKD 357 + Y + + + E L++A G R L ALET P G ++S D Sbjct: 310 FLSLEGY----IAARTLVEGLRRA--GRALTREGLIRALETISPANYDGGGFSQHFSRTD 363 Query: 358 HFG 360 H G Sbjct: 364 HSG 366 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,158,617 Number of extensions: 47166 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 10 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)