BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0052.p (139 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313297|ref|YP_003777389.1| 4-hydroxybenzoyl-CoA thioestera... 285 1e-78 >gi|300313297|ref|YP_003777389.1| 4-hydroxybenzoyl-CoA thioesterase [Herbaspirillum seropedicae SmR1] Length = 139 Score = 285 bits (728), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 138/139 (99%), Positives = 139/139 (100%) Query: 1 MSKTIIHPVRVEFGDCDPAGIVYFPNFFRWYDAASRNFFHECGVPPWRDTEKTRGIIGTP 60 MSKTIIHPVRVEFGDCDPAGIVYFPNFFRWYDAASRNFFHECGVPPWRDTEKTRGIIGTP Sbjct: 1 MSKTIIHPVRVEFGDCDPAGIVYFPNFFRWYDAASRNFFHECGVPPWRDTEKTRGIIGTP 60 Query: 61 VVDISSRFVRPATYGDRIEVHSTIVEWNDKTFVMQHEIKRDGELLCEGRDVRVFAIRHPD 120 VVDISSRFVRPATYGDRIEVHS+IVEWNDKTFVMQHEIKRDGELLCEGRDVRVFAIRHPD Sbjct: 61 VVDISSRFVRPATYGDRIEVHSSIVEWNDKTFVMQHEIKRDGELLCEGRDVRVFAIRHPD 120 Query: 121 DPARIKAIPIPADIREMCS 139 DPARIKAIPIPADIREMCS Sbjct: 121 DPARIKAIPIPADIREMCS 139 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.143 0.471 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 480,055 Number of extensions: 19105 Number of successful extensions: 60 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 1 Length of query: 139 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 63 Effective length of database: 1,260,010 Effective search space: 79380630 Effective search space used: 79380630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)