BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0051.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 432 e-122 gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 226 1e-60 gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 214 5e-57 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 92 4e-20 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 80 9e-17 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 432 bits (1111), Expect = e-122, Method: Compositional matrix adjust. Identities = 215/216 (99%), Positives = 215/216 (99%) Query: 1 MEAAQALADELRCHAVRCDITDSDSVEAALQAAQQTNGAPRILINCAGIGGAKRMVGKDG 60 MEAAQALADELRCHAVRCDITDSDSVE ALQAAQQTNGAPRILINCAGIGGAKRMVGKDG Sbjct: 39 MEAAQALADELRCHAVRCDITDSDSVETALQAAQQTNGAPRILINCAGIGGAKRMVGKDG 98 Query: 61 KPMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQA 120 KPMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQA Sbjct: 99 KPMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQA 158 Query: 121 GYAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKR 180 GYAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKR Sbjct: 159 GYAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKR 218 Query: 181 LGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 216 LGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR Sbjct: 219 LGKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 254 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 226 bits (575), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 117/215 (54%), Positives = 160/215 (74%), Gaps = 2/215 (0%) Query: 2 EAAQALADELRCHAVRCDITDSDSVEAALQAAQQTNGAPRILINCAGIGGAKRMVGKDGK 61 EA + LA EL VRCD++ + +A ++AA+ G+ R L+NCAGI A+++VGK G Sbjct: 40 EAGEPLAAELGGVFVRCDVSSEEDAQAVVRAAEAL-GSLRGLVNCAGIAPAEKLVGKSG- 97 Query: 62 PMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAG 121 P + F + +N+NL+G+FN+ R AA MA + A+ EGERGVI+ TASVAA+DGQ+GQ Sbjct: 98 PHTMALFMKTLNINLVGSFNMARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTA 157 Query: 122 YAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRL 181 YAASKG I AMTLP+AR+L++ GIRV+TIAPG+F TP++ +P+EV+ SL ++PFP RL Sbjct: 158 YAASKGAIVAMTLPMARELSRSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRL 217 Query: 182 GKAEEYAQLALHIATNLSLNGEVIRLDGALRLAPR 216 G+A EYAQLA I N+ LNGE IRLDGA+R+ P+ Sbjct: 218 GRAAEYAQLARTIIENVMLNGETIRLDGAIRMQPK 252 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 214 bits (544), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 107/201 (53%), Positives = 145/201 (72%), Gaps = 2/201 (0%) Query: 16 VRCDITDSDSVEAALQAAQQTNGAPRILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVN 75 V D+T S + A GA L+NCAGI ++++GK G L+ F R +N+N Sbjct: 56 VLTDVTSETSAQEVFGVASSM-GAVVGLVNCAGIAPGEKLLGKGGVHR-LDSFMRALNIN 113 Query: 76 LIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLP 135 L+G+FN+ RLAA ++A A A GERGVI+ TAS+AAFDGQ+GQ YAASKGG+ +MTLP Sbjct: 114 LVGSFNMARLAAQHISAGPADATGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLP 173 Query: 136 LARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRLGKAEEYAQLALHIA 195 +AR+LA++GIRVVTIAPG+ TP++ +PEEV +SL+ ++PFP RLGK E+A L +HI Sbjct: 174 MARELARHGIRVVTIAPGIIETPMMLGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMHIL 233 Query: 196 TNLSLNGEVIRLDGALRLAPR 216 N LNGEVIR+DGALR++ + Sbjct: 234 ENRYLNGEVIRMDGALRMSAK 254 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 92.0 bits (227), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 62/200 (31%), Positives = 101/200 (50%), Gaps = 25/200 (12%) Query: 14 HAVRCDITDSDSVEAALQAAQQTNGAPRILINCAGIGGAKRMVGKDG--KPMPLEDFSRI 71 HA ++++ +S +AA + + G +L+N AGI +DG + M +D+ + Sbjct: 56 HASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGIT-------RDGQFRKMSKDDWDAV 108 Query: 72 VNVNLIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITA 131 ++ NL FNV + E + E G I+ +SV GQ GQ Y+ +K GI Sbjct: 109 IDTNLNSLFNVTK------QVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHG 162 Query: 132 MTLPLARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQSLASSIPFPKRLGKAEEYAQLA 191 T+ LA+++A G+ V T++PG T ++ + EV + + S IP KRLG+ EE A + Sbjct: 163 FTMALAQEVATKGVTVNTVSPGYIGTDMVRAIRPEVLEKIVSGIPV-KRLGEPEEIASIV 221 Query: 192 LHIA---------TNLSLNG 202 IA ++ SLNG Sbjct: 222 AWIASSEGGYATGSDFSLNG 241 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 80.5 bits (197), Expect = 9e-17, Method: Compositional matrix adjust. Identities = 67/203 (33%), Positives = 100/203 (49%), Gaps = 23/203 (11%) Query: 16 VRCDITDSDSVEAALQAAQQTNGAPRILINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVN 75 V ++ +SV AAL Q + I++NCAGI K G + E F +++ VN Sbjct: 58 VEMNVASEESVRAALAGLTQLD----IVVNCAGII-------KRGAELETEVFEQVIAVN 106 Query: 76 LIGTFNVIRLAAARMAAAEALAEGERGVIVATASVAAFDGQVGQAGYAASKGGITAMTLP 135 L GT V AAA L RG IV TAS+ +F G GY+ASKGG+ +T Sbjct: 107 LTGTMRVC-------AAARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKS 159 Query: 136 LARDLAQYGIRVVTIAPGLFLTPLLYKLPEEVQQS--LASSIPFPKRLGKAEEYAQLALH 193 LA A GIRV +APG TPL L ++ ++ + + P R G+ E+ Q+ Sbjct: 160 LAIAYAADGIRVNAVAPGWIATPLTQALQDDPVRAGPILARTPL-GRWGQPEDVGQVVAF 218 Query: 194 IATNLS--LNGEVIRLDGALRLA 214 + + + + G ++ +DG +A Sbjct: 219 LCSPAASFMTGAIVPVDGGYLVA 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 616,515 Number of extensions: 23304 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 26 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)