BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0049.p (620 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase ... 1276 0.0 gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase ... 95 2e-20 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 65 1e-11 >gi|300313294|ref|YP_003777386.1| long chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 1276 bits (3303), Expect = 0.0, Method: Compositional matrix adjust. Identities = 619/620 (99%), Positives = 619/620 (99%) Query: 1 MNTPPRYRSFRFGIGGVDVVPGADGVSIVKTRQPLDAYPARLTDKLIHWATVDPDRTYMA 60 MNTPPRYRSFRFGIGGVDVVPGADGVSIVKTRQPLDAYPARLTDKLIHWATVDPDRTYMA Sbjct: 1 MNTPPRYRSFRFGIGGVDVVPGADGVSIVKTRQPLDAYPARLTDKLIHWATVDPDRTYMA 60 Query: 61 RRDQDGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV 120 RRDQDGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV Sbjct: 61 RRDQDGQWRRISYGQALQSARRIGQALLQRGLSAERPLLILSENDLEHAMLVLGCHYAGV 120 Query: 121 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAPEIEFVVTEAPPA 180 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAPEIEFVVTEAPPA Sbjct: 121 PYAPVSSAYSLLSKDYAKLRHAVQLLTPGLIFAAHGEKFAAAVNAVAPEIEFVVTEAPPA 180 Query: 181 GRECTLYAELEATRAGDEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQM 240 GRECTLYAELEATRAGDEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQM Sbjct: 181 GRECTLYAELEATRAGDEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQM 240 Query: 241 IVQTFPFLGEEPPILIDWLPWNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANL 300 IVQTFPFLGEEPPILIDWLPWNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANL Sbjct: 241 IVQTFPFLGEEPPILIDWLPWNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANL 300 Query: 301 HDISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA 360 HDISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA Sbjct: 301 HDISPTVYFNVPVGWEGIAHALEKDQALREKFYSRLRMQFYAGAALAQPVWDKLHATAEA 360 Query: 361 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN 420 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN Sbjct: 361 TCGERIVMCCGLGMTETSPSALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPN 420 Query: 421 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV 480 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV Sbjct: 421 VTPGYWRAPEQTAEAFDEEGYFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSV 480 Query: 481 GPLRALIAHEGAPYLQDAVITGQDRKEIGMLIVPNIERCRQLARLPAEASRAEVLNAAPV 540 GPLRALIAHEGAPYLQDAVITGQDRKEIGMLIVPNIERCRQLARLPAEASRAEVLNAAPV Sbjct: 481 GPLRALIAHEGAPYLQDAVITGQDRKEIGMLIVPNIERCRQLARLPAEASRAEVLNAAPV 540 Query: 541 RDFFQGVLDRLQSHATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVET 600 RDFFQGVLDRLQSHATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVET Sbjct: 541 RDFFQGVLDRLQSHATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVET 600 Query: 601 LYAGTDPAVLTVQAALSPAH 620 LYAGTDPAVLTVQ ALSPAH Sbjct: 601 LYAGTDPAVLTVQPALSPAH 620 >gi|300311882|ref|YP_003775974.1| long-chain acyl-CoA synthetase [Herbaspirillum seropedicae SmR1] Length = 653 Score = 94.7 bits (234), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 111/482 (23%), Positives = 184/482 (38%), Gaps = 92/482 (19%) Query: 197 DEVERAFAATGPETIVKFLFTSGSTKMPKAVINTQRMMCSNLQMIVQTFPFLGEEPPILI 256 D ++ AA P+ + L+TSG+T PK V ++ R M + +V+ F L E IL Sbjct: 175 DFFDQEIAAGSPDDVAIILYTSGTTGKPKGVCHSHRAMITTATTLVE-FDHLDENDDILC 233 Query: 257 DWLP------WNHTFGANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANLHDISPTVYFN 310 +LP + ++F H G L P+ T + +L +I PT YF Sbjct: 234 -YLPLAWVGDFLYSFAQQHVAGFCL------------NCPESPNTVMTDLREIGPTYYFA 280 Query: 311 VPVGWEGIAHA----LEKDQALREKFY--------------------------------- 333 P +E I +E L+ K + Sbjct: 281 PPRVYENILTQVMIRIEDAGWLKRKMFHGFMKVARRVGMRILDGKPGVSLLDRLQYGLGS 340 Query: 334 -------------SRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSPS 380 SRLR+ + G A+ ++D + I + GMTET + Sbjct: 341 LLVYGPLKNVLGMSRLRVAYTGGEAIGPDLFDFYRSLG-------INLKQLYGMTETCVT 393 Query: 381 ALFVADLEVQAGQIGIPTPGMEIKLVPNGDKIEIRYRGPNVTPGYWRAPEQTAEAFDEEG 440 +V+ +G P G+E+++ NG E+ R P + Y++ P+ TAEA D G Sbjct: 394 VCMQPSGDVKLDSVGRPMKGVEVRIDENG---EVLVRSPGLMKEYFKRPDATAEAIDANG 450 Query: 441 YFRSGDAVRWLDPAHPDRGFMFDGRVAEDFKLYTGTWVSVGPLRALIAHEGAPYLQDAVI 500 YF +GDA + H R + K+ +G+ + + + P++++AV Sbjct: 451 YFHTGDAGFFDSDGH----LKIIDRAKDVGKMASGSMFAPKYIENKLKF--FPFIKEAVT 504 Query: 501 TGQDRKE------IGMLIVPNIERCRQLARLPAEASRAEVLNAAPVRDFFQGVLDRLQSH 554 G R++ I M V N R LA A A +R+ + V L + Sbjct: 505 FGNGREQCMAFINIDMDAVGNWAERRNLAYSGYTDLAANPAVYALLRECVEKVNADLAAD 564 Query: 555 ATGSSNRVTRALVLVDPPSFDRGEITDKGSINQRAVLTHRAALVETLYAGTDPAVLTVQA 614 + +++ R L+L D E+T + + + A L+E LYAG + Q Sbjct: 565 PLLADSQIHRFLILHKELDPDDEELTRTRKVRRGFIADKYAVLIEALYAGRASQYIETQV 624 Query: 615 AL 616 Sbjct: 625 KF 626 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 66/249 (26%), Positives = 102/249 (40%), Gaps = 27/249 (10%) Query: 212 VKFL-FTSGSTKMPKAVINTQRMMCSN-LQMIVQTFPFLGEEPPI----LIDWLPWNHTF 265 + FL +T G+T + K + T R + +N LQ P L PI + LP H F Sbjct: 207 IAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIF 266 Query: 266 GANHNVGIVLYNGGTMYIDEGKPTPQGLATTLANLHDISPTVYFNVPVGWEGIAHALEKD 325 + G + P P+ + + L + V + +AL + Sbjct: 267 ALTVCNMLGTREGALNLL---IPNPRDIPGFVKELAKYKVNTFPAV----NTLYNALLNN 319 Query: 326 QALREKFYSRLRMQFYAGAALAQPVWDKLHATAEATCGERIVMCCGLGMTETSP-SALFV 384 +S R G A+ + V DK E G G++ETSP ++ Sbjct: 320 ADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIE------GYGLSETSPIASANP 373 Query: 385 ADLEVQAGQIGIPTPGMEIKL-------VPNGDKIEIRYRGPNVTPGYWRAPEQTAEAFD 437 ++ G IG+P P E + VP G EI RGP V PGYW+ P++TA+ Sbjct: 374 CTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMT 433 Query: 438 EEGYFRSGD 446 +G+F++GD Sbjct: 434 PDGFFKTGD 442 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,034,486 Number of extensions: 89466 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 3 Length of query: 620 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 529 Effective length of database: 1,188,985 Effective search space: 628973065 Effective search space used: 628973065 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)