BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0048.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine ... 527 e-151 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 95 7e-21 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 86 4e-18 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 79 3e-16 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 78 6e-16 >gi|300313293|ref|YP_003777385.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 259 Score = 527 bits (1358), Expect = e-151, Method: Compositional matrix adjust. Identities = 259/259 (100%), Positives = 259/259 (100%) Query: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH Sbjct: 1 MNQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEH 60 Query: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI Sbjct: 61 FCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 Query: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY Sbjct: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 Query: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD Sbjct: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 Query: 241 TKGRLAAFLDLKQNKVKPV 259 TKGRLAAFLDLKQNKVKPV Sbjct: 241 TKGRLAAFLDLKQNKVKPV 259 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 94.7 bits (234), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 78/253 (30%), Positives = 125/253 (49%), Gaps = 16/253 (6%) Query: 4 HSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLPE--GVKVAILRGHGEHF 61 + +LL + + +TLNR A RNAL + +R I E V+V ++ G+ + F Sbjct: 7 YEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAF 66 Query: 62 CAGLDLSELRERDT--TESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAH 119 AG DL+E+ +D T+ ++ W A IA P++A ++G +G G EL A Sbjct: 67 AAGADLNEMIHKDAIATQLDVRAQYWRA----IARFPKPILAAVNGYALGAGCELLMHAD 122 Query: 120 IRVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQ 179 I +A G PE G G GG+ R+ R +G M+L+G +SA++ QAG+ Sbjct: 123 IAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVA 182 Query: 180 YSVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTE----SLMAAVA 235 VD A +++ LA IA+ +P+ + + + ++ GL+ E SLMAA A Sbjct: 183 EVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASA 242 Query: 236 GSDPDTKGRLAAF 248 D + +AAF Sbjct: 243 ----DRQEGIAAF 251 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 72/257 (28%), Positives = 121/257 (47%), Gaps = 9/257 (3%) Query: 6 ELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCF--ENLPEGVKVAILRGHGEHFCA 63 E + ++ H + I L+R NAL+D L+ + + + E + I+ G + F A Sbjct: 4 ENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAA 63 Query: 64 GLDLSELRERDTTESLHH---SRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHI 120 G D+S + D + +R W E + R PVIA + G +GGG ELA Sbjct: 64 GADISAMAGYDYMDVFKGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCELAMMCDF 119 Query: 121 RVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQY 180 +A + +G PE + G+ G GG+ R+ R + A+ D+ LTGR++ AEE +AG+ Sbjct: 120 IIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVSR 179 Query: 181 SVDAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPD 240 V A +++A A IA V + R ++ ++ +G+ E + + D Sbjct: 180 IVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATED 239 Query: 241 TKGRLAAFLDLKQNKVK 257 K + AFL+ +Q K Sbjct: 240 QKEGMKAFLEKRQPVFK 256 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 67/266 (25%), Positives = 127/266 (47%), Gaps = 26/266 (9%) Query: 2 NQHSELLDIQQHGAIAEITLNRAAKRNALNDPLVRAIRDCFENLP--EGVKVAILRGHGE 59 + H ++L+ + I +TLNR NAL++ ++ A++ +++ V+ IL +G+ Sbjct: 3 DHHPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGK 62 Query: 60 HFCAGLDLSELRERDTTESLHHSRMWHAAFEQIAFGR---VPVIAVLHGAVIGGGLELAA 116 FCAG DL +++ T + ++ ++H + R VPVIA + G G +L A Sbjct: 63 AFCAGHDLRQMQA--TPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVA 120 Query: 117 AAHIRVAEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAG 176 + +A S + + GLF +V ++R + + R +M++TG+ + A G Sbjct: 121 TCDLAIAAESARFAVSGINVGLFC-STPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWG 179 Query: 177 -----IPQYSVDAGAGMDKARELAER----IARNAPMTNYGVMHVLPRIHDQSIQDGLMT 227 +P+ +D+ A ARE+ + IAR M M LP + + + +M Sbjct: 180 LINQAVPEQELDS-ATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAE--VMA 236 Query: 228 ESLMAAVAGSDPDTKGRLAAFLDLKQ 253 ++M DP+ + + AFLD ++ Sbjct: 237 CNIM------DPEAQEGITAFLDKRK 256 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 78.2 bits (191), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 69/255 (27%), Positives = 115/255 (45%), Gaps = 8/255 (3%) Query: 8 LDIQQHGAIAEITLNR----AAKRNALNDPLVRAIRDCFENLPEGVKVAILRGHGEHF-C 62 L +++ G A +TL+ R AL D L R +++ N + ++ G GE F Sbjct: 7 LKLEKTGHTAVLTLSNPPANTWTRAALAD-LTRLVKEL--NADRDIYTLVVTGEGEKFFS 63 Query: 63 AGLDLSELRERDTTESLHHSRMWHAAFEQIAFGRVPVIAVLHGAVIGGGLELAAAAHIRV 122 AG DL + D + +R + AFE ++ R IA ++G +GGGLE A A IR+ Sbjct: 64 AGADLKLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACDIRI 123 Query: 123 AEPSTFYGLPEGQRGLFVGGGGSVRISRLIGVARMTDMMLTGRVLSAEEGHQAGIPQYSV 182 AE LPE GL GG+ + L+G M+L G ++AE + G+ + V Sbjct: 124 AEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVEEVV 183 Query: 183 DAGAGMDKARELAERIARNAPMTNYGVMHVLPRIHDQSIQDGLMTESLMAAVAGSDPDTK 242 G ++A LA++ + +P + ++ + L TE + D + Sbjct: 184 PRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQDQR 243 Query: 243 GRLAAFLDLKQNKVK 257 + AFL+ + + K Sbjct: 244 EGVQAFLEKRSPQWK 258 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 860,570 Number of extensions: 39052 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 7 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)