BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0045.p (365 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313291|ref|YP_003777383.1| porin transmembrane protein [He... 731 0.0 gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspir... 315 4e-87 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 148 6e-37 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 144 1e-35 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 136 2e-33 >gi|300313291|ref|YP_003777383.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 365 Score = 731 bits (1888), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/365 (100%), Positives = 365/365 (100%) Query: 1 MTRKISLHAVALAAALCASSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSF 60 MTRKISLHAVALAAALCASSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSF Sbjct: 1 MTRKISLHAVALAAALCASSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSF 60 Query: 61 PSRIGFKGTEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQ 120 PSRIGFKGTEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQ Sbjct: 61 PSRIGFKGTEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQ 120 Query: 121 VNMTYLSQLKTDVMGPNLFAIGSIDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAG 180 VNMTYLSQLKTDVMGPNLFAIGSIDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAG Sbjct: 121 VNMTYLSQLKTDVMGPNLFAIGSIDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAG 180 Query: 181 PAGPAATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTD 240 PAGPAATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTD Sbjct: 181 PAGPAATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTD 240 Query: 241 QRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLD 300 QRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLD Sbjct: 241 QRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLD 300 Query: 301 VKNSPNDVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTG 360 VKNSPNDVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTG Sbjct: 301 VKNSPNDVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGAGKNQLGVMTG 360 Query: 361 IRHTF 365 IRHTF Sbjct: 361 IRHTF 365 >gi|300310614|ref|YP_003774706.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 369 Score = 315 bits (806), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 171/370 (46%), Positives = 242/370 (65%), Gaps = 13/370 (3%) Query: 6 SLHAVALAAALCASSAMAQS-SVTIYGIIDTGVVYTTNAN---ANGNSVFKMPSLTGSFP 61 +L A+ +AA L S+ ++Q+ SVT+YGIIDTGV Y +N + A + ++P+LT + P Sbjct: 3 ALKAI-IAALLTMSATLSQAQSVTLYGIIDTGVEYVSNVSNVGAGQQGLLRVPNLTATTP 61 Query: 62 SRIGFKGTEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQV 121 SRIG +G+EDLGGGL+++F+LESGFAPD+GA+ QGGR+FGR +YVGL + WGQ+ GRQ Sbjct: 62 SRIGLRGSEDLGGGLRSVFMLESGFAPDSGALNQGGRMFGRIAYVGLSDHWGQVSFGRQA 121 Query: 122 NMTYLSQLKTDVMGPNLFAIGSIDAYLPNARSDNAIGYLGNFSGFTVGATYSFGRDAAGP 181 M + L +D++G N++ ++D Y PNAR+DN+I Y G+F FT+G TYSFGRD Sbjct: 122 TMLLWATLDSDILGANVYGANALDNYRPNARADNSIAYRGSFGAFTLGGTYSFGRDTVNA 181 Query: 182 A-GPAATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTD 240 PA TNC GE AG+S ACR+ + ++ YD WG + +YD L G GA+ G++SS +D Sbjct: 182 GQNPAGTNCAGEQAGDSLACREWSLMVKYDTAAWGASLAYDTLRGGKGAAAGLSSSQLSD 241 Query: 241 QRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLD 300 R+ L Y +K+G G R + +A + S LYY G+SY L+ L L Q+ L Sbjct: 242 SRMLLGAYIKADRLKVGIGGSLRHNQGSALSK--SQLYYAGLSYDLTPTLNLAGQLYYLH 299 Query: 301 VKNSPNDVTLSVARLTYNLSKRTAVYGSLGYIKNGGTSSVALDAGGTVGA-----GKNQL 355 +S N L R TY+LS RTA+Y + G+I N G S++++ AGG+VG G NQ Sbjct: 300 YDDSANKAWLGALRATYSLSARTALYLTGGFIDNRGQSNLSVSAGGSVGTANPAPGGNQF 359 Query: 356 GVMTGIRHTF 365 G+M GIRH+F Sbjct: 360 GMMAGIRHSF 369 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 148 bits (374), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 137/383 (35%), Positives = 184/383 (48%), Gaps = 63/383 (16%) Query: 20 SAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFP-SRIGFKGTEDLGGGLQA 78 +A AQS+VTIYGI+DT + Y +NANA GNS +M + G+ SRIGFKGTEDLGGGL+A Sbjct: 18 AAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDN--GAIANSRIGFKGTEDLGGGLKA 75 Query: 79 MFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPNL 138 MF LE+GF+ DTGAM QGG LFGRQS+VGL+ +G++ +GRQ T L L D P Sbjct: 76 MFQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQ--NTPLFDLLADHFDPLT 133 Query: 139 FAIGSIDAYLPNA----RSDNAIGYLGNFSGFTVGATYSFGRDAAG-------------P 181 + +++LP A RS N + Y G+F A+++FG A Sbjct: 134 VGNYASNSWLPAAASLVRSPNMLRYDGDFGPVAASASWAFGEKAGSVRTGSQFSSALRYT 193 Query: 182 AGPAAT--NCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDI----LYG---NTGASNG 232 AGP A V+ + + + T W +++SYD ++G N +G Sbjct: 194 AGPLALGGGYQQTVSSTNSSLKHTT---------WNLSASYDFGVAKVFGGWLNIKDDSG 244 Query: 233 MTSSDNTDQRVTLNGYFMLGDVKIGGGVVDRRTRAAANTNTDSDLYYLGVSYPLSVALVL 292 +T S NG+ G G V R+ ++LG SY + AL L Sbjct: 245 LTGSVMAADGDVSNGWTTAGAANNGSTGVKRKDNG----------FFLGSSYKATNALTL 294 Query: 293 DAQVSRLDVKNSPNDVTLSV---ARLT------YNLSKRTAVYGSLGYIKNGGTSSVALD 343 KN T +V R T Y LSKRT +Y ++ Y K + L Sbjct: 295 TGAFYYDRSKNVSYVSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELT 354 Query: 344 AGGTVGAGKNQL-GVMTGIRHTF 365 GKN L V GIRH F Sbjct: 355 NSAN---GKNSLTTVGVGIRHIF 374 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 125/360 (34%), Positives = 174/360 (48%), Gaps = 37/360 (10%) Query: 20 SAMAQSSVTIYGIIDTGVVYTTNANANG-NSVFKMPSLTGSFPSRIGFKGTEDLGGGLQA 78 S AQSSVTIYG++DTGV Y NA NG SV +M S + SRIGF+GTEDLGGGL+A Sbjct: 18 SVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTS-GATNTSRIGFRGTEDLGGGLKA 76 Query: 79 MFVLESGFAPDTGAM-GQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQLKTDVMGPN 137 +F LE+GF D G+ G +LF RQS VGL+ +G+++ GR + TY D MG + Sbjct: 77 LFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGPFDPMGYS 136 Query: 138 LFAIGSIDAYLPNARSD-------NAIGYLGNFSGFTVGATYSFGRDAAGPAGPAATNCP 190 + A R D N + Y G FSG +GA Y F Sbjct: 137 ANYSWATTAGATGNRKDGMPTGVSNLVKYQGEFSGVKIGAMYGF---------------- 180 Query: 191 GEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTLNGYFM 250 GEVAG ++ + LG+ + V ++YD N A+ T D GY Sbjct: 181 GEVAGETRNGAKYGVGLGWGTGPFSVVATYDR---NNSATATNTPYDRATSAHLAAGYQP 237 Query: 251 LGDVKIGGGV-VDRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRLDVKNSPNDV- 308 K G +++ A + SD ++ G +Y + A+ L + ++KN ND Sbjct: 238 SEAWKFNLGYRYYKKSLATGAADLRSDFFWGGAAYKFTPAMTLIGAIYYQNIKNVANDAD 297 Query: 309 -TLSVARLTYNLSKRTAVYGSLGY--IKNGGTSSVALDAGGTVGAGKNQLGVMTGIRHTF 365 + RL Y +SKRT +Y S Y KNG ++ D G G +Q G+ G++H F Sbjct: 298 PIMYSLRLKYAMSKRTDLYLSTAYAKAKNGQLVGLSRD---DAGYGSSQFGMTLGMQHRF 354 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 136 bits (343), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 133/384 (34%), Positives = 173/384 (45%), Gaps = 70/384 (18%) Query: 9 AVALAAALCASSAMAQSSVTIYGIIDTGVVYTTNANANGNSVFKMPSLTGSFPSRIGFKG 68 AV +A+ LCA A+AQS+VT+YG+IDT + YT NA G G SR+GFKG Sbjct: 6 AVGVASLLCAGGALAQSNVTLYGVIDTYMGYT---NATGKGSVASLDSGGYQASRVGFKG 62 Query: 69 TEDLGGGLQAMFVLESGFAPDTGAMGQGGRLFGRQSYVGLKNSWGQLMLGRQVNMTYLSQ 128 +EDLGGGL+A F +E+GFA D+GAM RLF RQS+VGL N G+L LGRQ + +L Sbjct: 63 SEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSPGFLMI 122 Query: 129 LKTDVMGPNLFA--IGSIDAYLPNARSDNAIGYLGN-FSGFTVGATYSFGRDAAGPAGPA 185 + D +A + ++ AY P R DN IGYL GF V A Y G A G + Sbjct: 123 ARLDAFAGATYASFLNNVSAYTP--RYDNVIGYLSPVMRGFRVQAYYGLGEQTAPRNGLS 180 Query: 186 ATNCPGEVAGNSKACRQVTALLGYDGKGWGVTSSYDILYGNTGASNGMTSSDNTDQRVTL 245 GE LY +S +++DN R T Sbjct: 181 TAMLAGEYESGP-------------------------LYLGVSSSAQNSANDNITIRSTF 215 Query: 246 NGYFMLGDVKIGGGVV------DRRTRAAANTNTDSDLYYLGVSYPLSVALVLDAQVSRL 299 G G G G V AAA+ NT Y +Y LS L Q + Sbjct: 216 AG----GAYDYGSGKVFLGYYRGNNLGAAASANTPGSYY---SAYSLSANYRLTGQATLG 268 Query: 300 DVKNSPNDVTLS--VARL-----TYNLSKRTAVYGSLGYIKNGGTSSVALD--------- 343 D T + AR TY+LS+RT +Y + ++ N +S +L Sbjct: 269 AGYGWAKDSTGAGRNARQFSLIGTYDLSRRTMLYATYAHLANNNGASFSLAGAAPITKNA 328 Query: 344 --AGGTVGAGKNQLGVMTGIRHTF 365 +GGTV GV GIRH F Sbjct: 329 PVSGGTVN------GVQVGIRHLF 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,103,338 Number of extensions: 48660 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 14 Length of query: 365 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 279 Effective length of database: 1,212,660 Effective search space: 338332140 Effective search space used: 338332140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)