BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0036.p (410 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 830 0.0 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 536 e-153 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 439 e-124 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 302 4e-83 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 291 7e-80 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 830 bits (2143), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/410 (98%), Positives = 406/410 (99%) Query: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGL 60 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRG Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 61 DVAGAAPAEAEAAAAPASPDQSAATHVGAEADRASAVALREHAREVTRQMFNAARMGKMV 120 DVAGAAPA+AEAAAAPASPDQSAATH GA+ADRASAVALREHAREVTRQMFNAARMGKMV Sbjct: 61 DVAGAAPAKAEAAAAPASPDQSAATHAGADADRASAVALREHAREVTRQMFNAARMGKMV 120 Query: 121 ELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAA 180 ELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAA Sbjct: 121 ELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAA 180 Query: 181 CREAGLSGYLHDVGKAFIDDEILNKPGKLTTEEFALIKRHPELGYQYLIQTPDMPAYASD 240 CREAGLSGYLHDVGKAFIDDEILNKPGKLT EEFALIKRHPELGYQYLIQTPDMPAYASD Sbjct: 181 CREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYASD 240 Query: 241 VCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMAR 300 VCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMAR Sbjct: 241 VCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMAR 300 Query: 301 WEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGQHET 360 WEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQG HET Sbjct: 301 WEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPHET 360 Query: 361 TIGGVLDLMAWPRTDTIIGRGDEEWTRLRQQDAAHAHRPRDGAADQQRSC 410 TIGGVLDLMAWPRTDTIIGRGDEEWTRLRQQDAAHAHRPRDGAADQQRSC Sbjct: 361 TIGGVLDLMAWPRTDTIIGRGDEEWTRLRQQDAAHAHRPRDGAADQQRSC 410 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 536 bits (1381), Expect = e-153, Method: Compositional matrix adjust. Identities = 261/398 (65%), Positives = 307/398 (77%), Gaps = 7/398 (1%) Query: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGL 60 MLKKIKV DLVLGMYFCGFEA+WL HPFWK +FLL +S+L+QA ASGIAYCWID RGL Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 61 DVAGAAPAEAEAAAAP-----ASPDQSAATHVGAEADRASAVALREHAREVTRQMFNAAR 115 DVA EA +AAP SP+ A +A A+ALRE A++ T+ MF+ AR Sbjct: 61 DVA--QEEEAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEAR 118 Query: 116 MGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLG 175 MGK++++ +CV++VEEIA SV++ PNG LSVLRLKL DEYTYLHSV+VCAL++ LGRTLG Sbjct: 119 MGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTLG 178 Query: 176 LSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTTEEFALIKRHPELGYQYLIQTPDMP 235 L E ACREAGL G+LHDVGKAFI E+LNKPGKL+ EEF+ IK+HPELGY+YL Q P++P Sbjct: 179 LDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNVP 238 Query: 236 AYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAI 295 YA DVC HHHE++DG GYP+ L A +ITLY+RM A+CDVYDALTS+RPYK WDPAEAI Sbjct: 239 DYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEAI 298 Query: 296 SQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQ 355 S MA W GHFDR + LAFVKTLGIYPVGS+V+LESG SG VIRQN DLTRPVLKV H Sbjct: 299 SAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHYD 358 Query: 356 GQHETTIGGVLDLMAWPRTDTIIGRGDEEWTRLRQQDA 393 T +LDL+A P DTI+ RG EEW RL DA Sbjct: 359 DSMTVTDTEILDLLANPEADTIVARGGEEWARLSHLDA 396 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 439 bits (1130), Expect = e-124, Method: Compositional matrix adjust. Identities = 204/386 (52%), Positives = 276/386 (71%), Gaps = 5/386 (1%) Query: 2 LKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGLD 61 LKKI+VG L +GMYF GF+ +W+ PFW+T FL+ + S++ +ARA+G YCWID +G+D Sbjct: 4 LKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGID 63 Query: 62 VAGAAPAEAEAAAAPASPDQSAATHVGAEADRASAVALREHAREVTRQMFNAARMGKMVE 121 +AGA EA P + + AA + ++ A+ L E+A++ T+QMF AR+GK ++ Sbjct: 64 LAGA-----EADRLPETEEPEAAGSSSSRSELDDALRLYENAKQTTQQMFKQARLGKTLD 118 Query: 122 LPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAAC 181 + C +LVEEIA SV RD N LLS++RLK DEYTYLHS++VC L++ L RTLG+ + AC Sbjct: 119 VENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIAC 178 Query: 182 REAGLSGYLHDVGKAFIDDEILNKPGKLTTEEFALIKRHPELGYQYLIQTPDMPAYASDV 241 +EAGL+GYLHDVGKAFI EILNKP +L+ +E+ +I+RHP LGY YL +P Y DV Sbjct: 179 KEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDV 238 Query: 242 CRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMARW 301 C HHHE++DG GYP I++Y+RM AVCDVYDA+TSERPYK GWDPAE++S+MA W Sbjct: 239 CLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASW 298 Query: 302 EGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGQHETT 361 +GHFDR + LAFVK LGIYP+GS+V+L+SG + +VIRQN DLT+PV+K E Sbjct: 299 QGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVV 358 Query: 362 IGGVLDLMAWPRTDTIIGRGDEEWTR 387 ++DL A +TD I R ++W + Sbjct: 359 ESEIIDLSAPGQTDAIAERSGQDWLK 384 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 302 bits (773), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 166/407 (40%), Positives = 238/407 (58%), Gaps = 19/407 (4%) Query: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGL 60 MLK I L +GMY F +W P W+ FLL ++ DL +AS I ID+++G Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 61 DVA-----------GAAPAEAEAAAAPASPDQSAATHVGAEADRASAVALREHAREVTRQ 109 DV + AE A A P+ T + AE +RA+ + +T Sbjct: 61 DVQTDEAEQGNGVESSLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLT-- 118 Query: 110 MFNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIG 169 MF+ ARMGK +++ + LV EI SV R+ + ++S++RLK D YTY+HSV+VCA++I Sbjct: 119 MFSQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMIS 178 Query: 170 LGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTTEEFALIKRHPELGYQYLI 229 L LGLS ++AG++G LHD+GK + ILNKP KLT EEF ++ HP G++ L Sbjct: 179 LADQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQ 238 Query: 230 QTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGW 289 + P + DV HHHE++DG GYP L I++ ARM A+CDVYDA+TS+RPYK GW Sbjct: 239 ELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGW 298 Query: 290 DPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVL 349 DP+ +I MA+ +GHFD +L AFVK +GIYPVGS V +++G +V+ Q P L P++ Sbjct: 299 DPSRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLV 358 Query: 350 KVLHLQGQHETTIGGVLDLMAWPRTDTIIGRGD-EEW--TRL-RQQD 392 ++ + + ++DL + I+G D E+W TRL QQD Sbjct: 359 RIFYSTNKKIAVTPTLIDLRR--SANKIVGAVDPEKWGVTRLVEQQD 403 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 291 bits (745), Expect = 7e-80, Method: Compositional matrix adjust. Identities = 156/374 (41%), Positives = 214/374 (57%), Gaps = 6/374 (1%) Query: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGL 60 MLK I + L LGMY W++HPF + F + DLQ R I I+ ++G Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 61 DVAGAAPAEAEAAAAPASP------DQSAATHVGAEADRASAVALREHAREVTRQMFNAA 114 DV E +A + +++ A+ +R A + ++ MF+ A Sbjct: 61 DVTREELEEDDADQDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMFSEA 120 Query: 115 RMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTL 174 RMGK +++ LV EI SV R+ + L+S+ RLK D+YTYLHSV+VCA++I L L Sbjct: 121 RMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQL 180 Query: 175 GLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTTEEFALIKRHPELGYQYLIQTPDM 234 G++ ++AG++G LHDVGK + +ILNKP KLT EEFA ++ H G+ L Q + Sbjct: 181 GMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGI 240 Query: 235 PAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEA 294 A DV HHHE++DG GYP L A+SI++ A+M AVCDVYDA+TS RPYK GWDPA + Sbjct: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 Query: 295 ISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHL 354 I MA GHFD + AFVK +GIYP GS V L+SG GVV+ Q P L P +KV + Sbjct: 301 IRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYS 360 Query: 355 QGQHETTIGGVLDL 368 ++DL Sbjct: 361 TANRMPLESEIIDL 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,304,888 Number of extensions: 54096 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 10 Length of query: 410 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 323 Effective length of database: 1,207,925 Effective search space: 390159775 Effective search space used: 390159775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)