BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0033.p (318 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 642 0.0 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 238 5e-64 gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 206 2e-54 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 79 6e-16 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 67 3e-12 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 642 bits (1656), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/318 (99%), Positives = 316/318 (99%) Query: 1 MTTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHE 60 MTTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHE Sbjct: 1 MTTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHE 60 Query: 61 QHRYQIGVLRDVNSRGGSVAVHEALKLGDILTISTPRNQFALVPAQHSLLLAGGIGVTPI 120 QHRYQIGVLRDVNSRGGSVAVHEALKLGDILTIS PRNQFALVPAQHSLLLAGGIGVTPI Sbjct: 61 QHRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVPAQHSLLLAGGIGVTPI 120 Query: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL 180 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL Sbjct: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL 180 Query: 181 AQADPATHLYVCGPVGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADGNGAFEVKLASS 240 AQADPATHLYVCGP GFIAHVVDTARARGWPEAQVHFEYFGAAPVKADGNGAFEVKLASS Sbjct: 181 AQADPATHLYVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAAPVKADGNGAFEVKLASS 240 Query: 241 GQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND 300 GQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND Sbjct: 241 GQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAND 300 Query: 301 QFTPCCSRARSALLVLDL 318 QFTPCCSRARSALLVLDL Sbjct: 301 QFTPCCSRARSALLVLDL 318 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 238 bits (607), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 147/319 (46%), Positives = 187/319 (58%), Gaps = 5/319 (1%) Query: 4 LQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQHR 63 +++ V +E DI + LV DGA LPA+ GAHIDV P G++RQYSLC ++ Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 64 YQIGVLRDVNSRGGSVAVHEALKLGDILTISTPRNQFAL-VPAQHSLLLAGGIGVTPILC 122 Y I V R+ SRGGS A+H+ +K G L + TPRN FAL A LL GIGVTP+L Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 123 MAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALLAQ 182 MA L A F +HY R+ AF + + + FAA V HY A + L L Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 183 ADPATHLYVCGPVGFIAHVVDTARARGWPEAQVHFEYF---GAAPVKADGNGAFEVKLAS 239 H+Y CGP F+ VV A A EA +H E+F AAP A G+GAFEVKLAS Sbjct: 181 GSKEAHVYTCGPAPFMDAVVKCASATRSEEA-IHLEHFQAAPAAPAAAGGDGAFEVKLAS 239 Query: 240 SGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAAN 299 SG+V IP ++ +L G+ + SC +GICGTC+ +LEGEPDHRD L+ +E+AAN Sbjct: 240 SGKVVQIPVGIPIVDILAREGIVIDTSCREGICGTCVVPLLEGEPDHRDNCLSKKEKAAN 299 Query: 300 DQFTPCCSRARSALLVLDL 318 DQ C SRARS +LVLDL Sbjct: 300 DQICTCVSRARSGMLVLDL 318 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 206 bits (523), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 139/329 (42%), Positives = 185/329 (56%), Gaps = 15/329 (4%) Query: 2 TTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQ 61 TTL ++V A R+ A DI LELV+ DGAALP +T GAH+ + L GLVR YSLCN P ++ Sbjct: 14 TTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQR 73 Query: 62 HRYQIGVLRDVNSRGGSVAVHEALKLGDILTISTPRNQFALVP-AQHSLLLAGGIGVTPI 120 Y I V R+ +SRGGS +HE ++ G ++ S P N F L+ + LLLA GIG+TP+ Sbjct: 74 DCYLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPL 133 Query: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAASGFAARVVHHYDDGEAAQKLDLDALL 180 MA LA G +HY ARS A AF +AA G +H DG A + + A L Sbjct: 134 HAMAAELATQGRRHRLHYFARSLAHAAFFHELAA-GSQGMSLHLGLDG-AETEHAIAAAL 191 Query: 181 AQADPATHLYVCGPVGFIAHVVDTARARGWPEAQVHFEYFGAA-----------PVKADG 229 A A ++ LYVCGP FI A+ GW E +HFE F A PV+A Sbjct: 192 AAAPRSSPLYVCGPSPFIDAARRHAQLAGWREQDIHFERFAAPAMPAAEADPARPVQAP- 250 Query: 230 NGAFEVKLASSGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQ 289 + FE+ L SG + P ++++ + GV + SC +G CG+C + VLEG+P HRD Sbjct: 251 DSTFELVLQRSGLRCQVLPGQSIVAAAAQVGVVIGTSCGEGFCGSCESTVLEGQPWHRDS 310 Query: 290 YLTDEERAANDQFTPCCSRARSALLVLDL 318 L+ ERA+ + PC SR LVLDL Sbjct: 311 VLSAAERASGRRILPCVSRCAGTRLVLDL 339 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 78.6 bits (192), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 79/305 (25%), Positives = 135/305 (44%), Gaps = 39/305 (12%) Query: 48 LVRQYSLCNAPHEQHRYQIGVLRDVNSRGG--SVAVHEALKLGDILTISTPRNQFALVPA 105 L R YS+C+A E+ + ++ + R + GG S +E+ G + + P F VP Sbjct: 53 LRRSYSICSAVQER-QLRVAIKR---APGGLFSNWANESFVPGQRIDVMPPMGHFN-VPL 107 Query: 106 Q-----HSLLLAGGIGVTPILCMAEA--LAAAGASFEMHYCARSPARQAFRERIA--ASG 156 + H L A G G+TP++ + + L+ + F + Y R+ + F+E + Sbjct: 108 EAGNRKHYLAFAAGSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDA 167 Query: 157 FAARVVHHYDDGEAAQKLDL----------DALLAQADPATHL---YVCGPVGFIAHVVD 203 + R Y Q ++L DA A + ++CGP + V Sbjct: 168 YLERFNVVYVMSREQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAA 227 Query: 204 TARARGWPEAQVHFEYFGAA-PVKAD------GNGAFEVKLASSG--QVYTIPPER-TVI 253 + +A G P+ Q+ E F A+ P +A G EV + G V+T+ E+ +V+ Sbjct: 228 SLQAHGLPKTQIKTELFAASTPPRAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVL 287 Query: 254 QVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSAL 313 + +HG+D+ SC+ G+C TC +V+EG+ D Y ++ A C S S Sbjct: 288 EAGLKHGIDLRYSCKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDK 347 Query: 314 LVLDL 318 L+LD Sbjct: 348 LLLDF 352 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 67/240 (27%), Positives = 110/240 (45%), Gaps = 30/240 (12%) Query: 8 VAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLV------RQYSLC---NAP 58 V+ + E+ +I + V DG L F G +I + + +V RQYSL Sbjct: 160 VSRKVAESEEITSFHFVPQDGGELLDFAPGQYIGLRM---VVDGEEQRRQYSLSALLRQA 216 Query: 59 HEQHRYQIGVLRDVNSRGGSVA--VHEALKLGDILTISTPRNQFALVPAQHSL-LLAGGI 115 + +Y+I V R+ GG V+ +H+ + +G ++ + P +F L+ + L L++GG+ Sbjct: 217 GQPPQYRISVKREP---GGKVSRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGV 273 Query: 116 GVTPILCMAEALAAAGASFEMHYCARSPARQAFR---ERIAASGFAARVVHHYD---DGE 169 G+TP++ M A +G + AR AFR E +AA ++ Y+ G+ Sbjct: 274 GITPMMSMLAAALPSGRPIHFIHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGD 333 Query: 170 A---AQKLDLDALLAQ---ADPATHLYVCGPVGFIAHVVDTARARGWPEAQVHFEYFGAA 223 A A L +LL Q +P Y GP F+ + G P AQ +E+FG A Sbjct: 334 AAPHATGLIERSLLQQWLPGNPDLDAYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPA 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 959,330 Number of extensions: 38621 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 6 Length of query: 318 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 233 Effective length of database: 1,217,395 Effective search space: 283653035 Effective search space used: 283653035 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)