BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0031.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro... 632 0.0 gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr... 131 8e-32 gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum se... 70 3e-13 >gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydrolase/4-carboxymuconolactone decarboxylase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 632 bits (1631), Expect = 0.0, Method: Compositional matrix adjust. Identities = 316/316 (100%), Positives = 316/316 (100%) Query: 105 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL 164 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL Sbjct: 1 MIQAAVYAGVPAGNTAFTHAQKILREVGEQIGYAVAPQAPAASVHPGVGREGRTASSPAL 60 Query: 165 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL 224 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL Sbjct: 61 HYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGL 120 Query: 225 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA 284 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA Sbjct: 121 YSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDA 180 Query: 285 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC 344 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC Sbjct: 181 AREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCC 240 Query: 345 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE 404 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE Sbjct: 241 HAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVE 300 Query: 405 QPALFAELVCGFIDGI 420 QPALFAELVCGFIDGI Sbjct: 301 QPALFAELVCGFIDGI 316 >gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydrolase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 131 bits (330), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 102/256 (39%), Positives = 127/256 (49%), Gaps = 7/256 (2%) Query: 164 LHYTVREPRNGKAPRHTVVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADG 223 LHY + G A +VLS++LGT L MWD Q RV+ YD RGHG SE G Sbjct: 11 LHYQLE----GDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQSEVTPG 66 Query: 224 LYSMADLADDAARLLRELDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPD 283 S+A L D LL L + GLSMGG LA+ HP + L+L NT + Sbjct: 67 ECSIAQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNTGAKIGT 126 Query: 284 AAREAWQQRIATVRAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGC 343 A EAW RI TVR EG+ IA AV+ R+ + A V L+ T A GY Sbjct: 127 A--EAWNSRIETVRREGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEGYAAA 184 Query: 344 CHAVGTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAV 403 C AV D + IR PTL+IAG D TP A AQ + I GA+ V A+H+S Sbjct: 185 CAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQY-VEFDAAHISNQ 243 Query: 404 EQPALFAELVCGFIDG 419 +Q A F + V F+ G Sbjct: 244 QQAAAFTQAVVQFLTG 259 >gi|300313799|ref|YP_003777891.1| arylesterase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 70.5 bits (171), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 74/254 (29%), Positives = 104/254 (40%), Gaps = 16/254 (6%) Query: 181 VVLSHALGTDLMMWDGLANQLAA-DCRVIAYDHRGHGSSEKADGLYSMADLADDAARLLR 239 VV H D MW+ N LA RVIAYD RG G S + Y ADD A L+ Sbjct: 23 VVFGHGWPLDGDMWEYQMNFLAERGYRVIAYDRRGFGRSSQPWSGYDYDTFADDLAELME 82 Query: 240 ELDSGPVVWVGLSMGGMVGQELALRHPG--LVRALVLANTTSAY---PDAAREAWQQRIA 294 LD VG SMGG RH + +A +L T + PD + + A Sbjct: 83 HLDLQGATLVGFSMGGGDVARYIGRHGSKRVAKAALLGAVTPIFGQAPDFPQGVPAEVFA 142 Query: 295 TV-------RAEGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAV 347 + RA+ I A G + +A+ + G + C A Sbjct: 143 GIKEGLLKDRAQFISDFAAVFFGTNRPGKADAVSPGVLAQTFNIAMLASLKGTLDCVTAF 202 Query: 348 GTVDTAARLGSIRVPTLVIAGELDQGTPVAMAQALV-DGISGARLAVIAGASH-VSAVEQ 405 D A + PTLVI G+ DQ P+ LV + + GA+L + +GA H + A + Sbjct: 203 SGTDFRADIKKFNFPTLVIHGDDDQIVPLQSTGKLVAEMVPGAQLKIYSGAPHALCATHK 262 Query: 406 PALFAELVCGFIDG 419 + A+L+ F+ G Sbjct: 263 DQVNADLLA-FLKG 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,407,637 Number of extensions: 61104 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 4 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)