BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0031.0028.p
         (230 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase...   477   e-136
gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy...    67   1e-12
gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba...    63   2e-11
gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba...    60   1e-10

>gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase
           beta chain protein [Herbaspirillum seropedicae SmR1]
          Length = 230

 Score =  477 bits (1228), Expect = e-136,   Method: Compositional matrix adjust.
 Identities = 229/230 (99%), Positives = 230/230 (100%)

Query: 1   MQFDAIEPGVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTK 60
           MQFDAIEPGVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTK
Sbjct: 1   MQFDAIEPGVYPELIYPPYKSTVKRGPTQAPLRVRDEIATGTNLFASPKLILPHDMDLTK 60

Query: 61  QGRGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFG 120
           QG+GEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFG
Sbjct: 61  QGKGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPNFTGFG 120

Query: 121 KMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFA 180
           KMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFA
Sbjct: 121 KMLTDDNGRYRFVSIKPGPYPWGNHHKAWRPAHIHFSLFGNVYAQRLVTQMYFPNDPLFA 180

Query: 181 YDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNATPMGI 230
           YDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNATPMGI
Sbjct: 181 YDPIFQSIPDEAARQRLISRFSLEETVDDKMLGYEFDIVLRGRNATPMGI 230


>gi|300313016|ref|YP_003777108.1|
           6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum
           seropedicae SmR1]
          Length = 296

 Score = 66.6 bits (161), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 47/146 (32%), Positives = 67/146 (45%), Gaps = 25/146 (17%)

Query: 53  PHDMDLTKQGRGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPL 112
           P   D+    +GEP      V+G V   +G+P+ ++ +EVWQ +  G Y    D   A L
Sbjct: 121 PLGADIANGAKGEP----CFVSGSVRGRNGRPLAHARMEVWQADENGFY----DVQYADL 172

Query: 113 DPNFTGFGKMLTDDNGRYRFVSIKPGPYP-------------WGNHHKAWRPAHIHFSLF 159
           D  +   G + TD  GRY F SI   PYP              G H   WRPAH+HF + 
Sbjct: 173 D-QYRARGTLFTDAEGRYHFRSILANPYPIPHDGPVGRMLEKLGRH--PWRPAHLHFRIQ 229

Query: 160 GNVYAQRLVTQMYFPNDPLFAYDPIF 185
              Y + L+T ++  +D     D +F
Sbjct: 230 AEGY-ETLITHVFRRDDVYLDSDAVF 254


>gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 303

 Score = 63.2 bits (152), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 48/132 (36%), Positives = 66/132 (50%), Gaps = 19/132 (14%)

Query: 68  GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRY-FHKKDQHDAPLDPNFTGFGKMLTDD 126
           G+ + + G+V DE GKP+ N+L+EVW  N  GRY F   DQ  +P +   +    + TD+
Sbjct: 125 GDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQ--SPFNLRCS----IRTDE 178

Query: 127 NGRYRFVSIKPGPY---PWGNHHK--------AWRPAHIHFSLFGNVYAQRLVTQMYFPN 175
            GRYRF S  P  Y   P G   +          RPAHIHF +    Y ++L TQ+    
Sbjct: 179 QGRYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGY-RKLTTQINIAG 237

Query: 176 DPLFAYDPIFQS 187
           DP    D  F +
Sbjct: 238 DPYLWDDFAFAT 249


>gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 311

 Score = 60.5 bits (145), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 46/136 (33%), Positives = 60/136 (44%), Gaps = 33/136 (24%)

Query: 68  GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPLDPN-----FTGFGKM 122
           G+ I++ G V   DGKP+  + +EVW  N  G Y H         DP      F     +
Sbjct: 131 GQTIIMHGTVYGADGKPMPGAQVEVWHANTKGFYSH--------FDPTGEQKPFNMRRTI 182

Query: 123 LTDDNGRYRFVSIKPGPY-------------PWGNHHKAWRPAHIHFSLFGNVYAQRLVT 169
           +TD  GRY+F SI P  Y               G H    RPAHIHF +    + ++L T
Sbjct: 183 ITDAQGRYKFRSIMPTAYGCPPAGPTQGLLNALGRHGN--RPAHIHFFVTAEGH-RKLTT 239

Query: 170 QMYFPNDPL----FAY 181
           Q+    DPL    FAY
Sbjct: 240 QINIEGDPLIYDDFAY 255


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.141    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 816,585
Number of extensions: 35961
Number of successful extensions: 68
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 63
Number of HSP's successfully gapped: 4
Length of query: 230
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 148
Effective length of database: 1,231,600
Effective search space: 182276800
Effective search space used: 182276800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 129 (54.3 bits)