BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0031.0025.p
         (388 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313270|ref|YP_003777362.1| 4-hydroxybenzoate 3-monooxygena...   796   0.0  
gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate ...    64   2e-11

>gi|300313270|ref|YP_003777362.1| 4-hydroxybenzoate 3-monooxygenase
           [Herbaspirillum seropedicae SmR1]
          Length = 388

 Score =  796 bits (2057), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 387/388 (99%), Positives = 388/388 (100%)

Query: 1   MRTQVAIIGAGPAGLLLSHLLHLKGIESVVLETRSREEIESTIRAGVLEQGTMDILTETG 60
           MRTQVAIIGAGPAGLLLSHLLHLKGIESVVLETRSREEIESTIRAGVLEQGTMDILTETG
Sbjct: 1   MRTQVAIIGAGPAGLLLSHLLHLKGIESVVLETRSREEIESTIRAGVLEQGTMDILTETG 60

Query: 61  VGERMKREGALHHGIELAFGGRRHRIDLTELTGQAITVYAQHEVIKDLVAARLAAQGQLL 120
           VGERMKREGALHHGIELAFGGRRHRIDLTELTGQAITVYAQHEVIKDLVAARLAAQGQLL
Sbjct: 61  VGERMKREGALHHGIELAFGGRRHRIDLTELTGQAITVYAQHEVIKDLVAARLAAQGQLL 120

Query: 121 FSVSGTSIEGVETDKPRVRFMHEGEQHTLEADFIAGCDGFHGVSRPAIPESKRQDYQRIY 180
           FSVSGTSIEGVETDKPRVRFMHEGEQHTLEADFIAGCDGFHGVSRPAIP+SKRQDYQRIY
Sbjct: 121 FSVSGTSIEGVETDKPRVRFMHEGEQHTLEADFIAGCDGFHGVSRPAIPDSKRQDYQRIY 180

Query: 181 PFGWFGVLVEAPPSSDELIYAQHERGFVLVSTRSPTVQRLYFQCDPKDSVDNWSDDRIWN 240
           PFGWFGVLVEAPPSSDELIYAQHERGFVLVSTRSPTVQRLYFQCDPKDSVDNWSDDRIWN
Sbjct: 181 PFGWFGVLVEAPPSSDELIYAQHERGFVLVSTRSPTVQRLYFQCDPKDSVDNWSDDRIWN 240

Query: 241 EFHTRLENGDGWRLKEGKIFQKGIIGMRSFVSTPMQHGRLFLAGDAAHIVPPTGAKGLNL 300
           EFHTRLENGDGWRLKEGKIFQKGIIGMRSFVSTPMQHGRLFLAGDAAHIVPPTGAKGLNL
Sbjct: 241 EFHTRLENGDGWRLKEGKIFQKGIIGMRSFVSTPMQHGRLFLAGDAAHIVPPTGAKGLNL 300

Query: 301 AVGDVKRLAQGIDDFYRSASEAGLASYTEQALKRIWRAEYFSWWMTSMLHTFEDASPFQR 360
           AVGDVKRLAQGIDDFYRSASEAGLASYTEQALKRIWRAEYFSWWMTSMLHTFEDASPFQR
Sbjct: 301 AVGDVKRLAQGIDDFYRSASEAGLASYTEQALKRIWRAEYFSWWMTSMLHTFEDASPFQR 360

Query: 361 QIQRAELENVVNSRALSTALAENYVGAF 388
           QIQRAELENVVNSRALSTALAENYVGAF
Sbjct: 361 QIQRAELENVVNSRALSTALAENYVGAF 388


>gi|300313232|ref|YP_003777324.1| 3-(3-hydroxyphenyl) propionate
           hydroxylase [Herbaspirillum seropedicae SmR1]
          Length = 544

 Score = 63.9 bits (154), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 73/318 (22%), Positives = 138/318 (43%), Gaps = 20/318 (6%)

Query: 1   MRTQVAIIGAGPAGLLLSHLLHLKGIESVVLETRSREEIESTIRAGVLEQGTMDILTETG 60
           M+ QV I+GAGP G+ L +LL   GI ++++E     ++    RA  ++   +      G
Sbjct: 15  MQAQVLIVGAGPVGVTLGNLLGTYGISTLLIERNL--DVLDYPRAVGIDDEALRTFQSAG 72

Query: 61  VGERMKREGALHHGIELAFGGRRHRIDLTELTGQAITVYAQHEVIKDLVAARLAAQGQLL 120
           + + + R+   +  + +    RR   ++ + T +    Y ++   + L  A L    +  
Sbjct: 73  MADAIVRDVIQNVPMRMYTADRRCFAEI-QPTTREFGWYRRNLFSQPLGEATLRLGLRRF 131

Query: 121 FSVS---GTSIEGVETDKPRVR---FMHEGEQHTLEADFIAGCDGFHGVSRPAIPESKRQ 174
             VS   G  +  +  D+  V+       G Q TL A ++ GCDG     R  I +   +
Sbjct: 132 EHVSLRSGVELLDLVQDEAGVQASIIDEHGAQATLRAQYVVGCDGGRSTVREKILKLPYE 191

Query: 175 DYQRIYPFGWFGVLVEAPP--SSDELIYAQHERGFVLVSTRSPTVQRLYFQCDP-KDSVD 231
              + +P  W  +  +  P  +    ++ + +R +V ++     V+R  F   P +D   
Sbjct: 192 G--KTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPY-GVRRWEFMLFPGEDGEQ 248

Query: 232 NWSDDRIWNEFHTRLENGDGWRLKEGKIFQKGIIGMRSFVSTPMQHGRLFLAGDAAHIVP 291
             + +++       + + D   +   +++        S V+  +  GR+ LAGDAAHI P
Sbjct: 249 MLAPEKVRELLREHVRDVDALNIIRARVYTHN-----SRVAGSLVCGRICLAGDAAHITP 303

Query: 292 PTGAKGLNLAVGDVKRLA 309
           P   +GLN  + D   LA
Sbjct: 304 PWIGQGLNAGLRDAFNLA 321


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,239,040
Number of extensions: 53782
Number of successful extensions: 160
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 160
Number of HSP's successfully gapped: 2
Length of query: 388
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 301
Effective length of database: 1,207,925
Effective search space: 363585425
Effective search space used: 363585425
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)