BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0020.p (895 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 compo... 1801 0.0 gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 compo... 962 0.0 >gi|300313265|ref|YP_003777357.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 1801 bits (4666), Expect = 0.0, Method: Compositional matrix adjust. Identities = 876/893 (98%), Positives = 883/893 (98%) Query: 3 MSRPSDSDIARDIDIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDA 62 MSRPSDSDIARDIDIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDA Sbjct: 1 MSRPSDSDIARDIDIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDA 60 Query: 63 RNTPYVNTIRPEQEPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFA 122 RNTPYVNTIRPEQEPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFA Sbjct: 61 RNTPYVNTIRPEQEPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFA 120 Query: 123 SAADLFEVGFNHFFRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEA 182 SAADLFEVGFNHFFRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEA Sbjct: 121 SAADLFEVGFNHFFRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEA 180 Query: 183 KRVGRQGLSSYPHPWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWG 242 KRVGRQGLSSYPHPWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWG Sbjct: 181 KRVGRQGLSSYPHPWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWG 240 Query: 243 VFGDGEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAG 302 VFGDGEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAG Sbjct: 241 VFGDGEMDEPESLAALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAG 300 Query: 303 WNVIKLLWGSDWDGLFARDKDGELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRA 362 WNVIKLLWGSDWDGLFARDKDGELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRA Sbjct: 301 WNVIKLLWGSDWDGLFARDKDGELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRA 360 Query: 363 IGATLTDEEINRLRRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTT 422 IGATLTDEEINRLRRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTT Sbjct: 361 IGATLTDEEINRLRRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTT 420 Query: 423 HQQKKLDEESLLQFRDRFKLPLTDEQCRALAFYKPADDSPEMKHLHARRAALGGYLPRRQ 482 HQQKKLDEESLLQFRDRFKLPL+DEQCRALAFYKPADDSPEMKHLHARRAAL GYLPRRQ Sbjct: 421 HQQKKLDEESLLQFRDRFKLPLSDEQCRALAFYKPADDSPEMKHLHARRAALSGYLPRRQ 480 Query: 483 AGQARRAVPPLAAHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTF 542 AGQARRAVPPLAAHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTF Sbjct: 481 AGQARRAVPPLAAHSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTF 540 Query: 543 GMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYS 602 GMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYS Sbjct: 541 GMANLFRQVGIYSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYS 600 Query: 603 VHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSL 662 VHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSL Sbjct: 601 VHGTPMLPFYIYYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSL 660 Query: 663 VTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEGVQE 722 VTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEGVQ+ Sbjct: 661 VTAASIPNCVSYDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEGVQD 720 Query: 723 GILRGMYCLGRKPGAQVRLLGSGPILKEAVAASALLQQHWQIEAEVWSVTSYTELARDGV 782 GILRG+YCL RKP AQ RLLGSGPILKEAVAA+ALLQQHWQIEAEVWSVTSYTELARDGV Sbjct: 721 GILRGLYCLARKPQAQARLLGSGPILKEAVAAAALLQQHWQIEAEVWSVTSYTELARDGV 780 Query: 783 AAQRAQRLSGSDGEPYVTAQLRDSAAPVIAVSDYIRTLPESIRAFVPAPYVTLGTDGFGR 842 AAQRAQRL+GS PYVT QL+ SA PVIAVSDYIRTLPESIRAFVPAPYVTLGTDGFGR Sbjct: 781 AAQRAQRLAGSAALPYVTQQLQGSAVPVIAVSDYIRTLPESIRAFVPAPYVTLGTDGFGR 840 Query: 843 SDTRVNLRDFFEIDARWIAYTALCEVWGNDRARLQEAARTLGIDTAKPLSSTV 895 SDTRVNLRDFFEIDARWIAYTALCEVWG+DRARLQEAARTLGIDTAKPLSSTV Sbjct: 841 SDTRVNLRDFFEIDARWIAYTALCEVWGDDRARLQEAARTLGIDTAKPLSSTV 893 >gi|300310785|ref|YP_003774877.1| pyruvate dehydrogenase E1 component protein [Herbaspirillum seropedicae SmR1] Length = 898 Score = 962 bits (2487), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/892 (53%), Positives = 628/892 (70%), Gaps = 20/892 (2%) Query: 16 DIDSQETQEWLQALQSVLAEAGPERARFLLSRLSAAAQQWGVNWRDARNTPYVNTIRPEQ 75 D D ETQEWL AL++V+ + GP+RA +L+ R+ A++ G + + NT YVNTI +Q Sbjct: 15 DPDVMETQEWLDALEAVIEKEGPDRAHYLMERMVDLARRRGAHIPFSSNTAYVNTIPADQ 74 Query: 76 EPPFPGGSDAQAIEERIASIMRWNALAMVVRANRAYGELGGHIASFASAADLFEVGFNHF 135 PG + EER+ S MRWNA+AMVV+ANR G+LGGH++SFAS A++ +GFNHF Sbjct: 75 GEHCPGNLE---YEERLRSWMRWNAMAMVVKANRVDGDLGGHLSSFASLANMLGIGFNHF 131 Query: 136 FRARDEQFAGDVVYFQPHSAPGIYARAFLEGALSEDDLAYYRREIEAKRVGRQGLSSYPH 195 + A E GD++Y Q HS+PGIYARAFLEG L+ED L ++RRE + GLSSYPH Sbjct: 132 WHAPTEDHGGDLLYIQGHSSPGIYARAFLEGRLTEDQLIHFRREADG-----HGLSSYPH 186 Query: 196 PWLMPQFWQFPTGSMGIGPINAIYQARFLRYMEHRGLLQDQGRKVWGVFGDGEMDEPESL 255 P LMP+FWQFPT SMG+GP+ AIYQARFL+Y+ RG+ + RKVW GDGEMDEPES+ Sbjct: 187 PKLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHARGIAKTDNRKVWAFCGDGEMDEPESM 246 Query: 256 AALSLASREKLDNLIFVVNCNLQRLDGPVRGNGHIVDELETLFAGAGWNVIKLLWGSDWD 315 A+ +A REKL+NL+ VVNCNLQRLDGPVRGNG I+ ELE+ F GAGWNV+K++WGS WD Sbjct: 247 GAIGMAGREKLNNLVIVVNCNLQRLDGPVRGNGKIIQELESDFRGAGWNVVKVIWGSGWD 306 Query: 316 GLFARDKDGELVDALNRTVDGQLQTFAANDGAFNRQHFFGQTPGTRAIGATLTDEEINRL 375 L A+DKDG L + TVDG+ Q + A DGA+ R+HFFG+ P + + ++D++I RL Sbjct: 307 ELLAKDKDGILQKVMMETVDGEYQNYKAKDGAYVRKHFFGKHPKLLEMVSKMSDDDIWRL 366 Query: 376 RRGGHDMKKIYSAYQAAARHRGQPTVILAQTKKGYGMGAAGEGKMTTHQQKKLDEESLLQ 435 RGGHD KIY+A++ A + QPTVILA++ KGYG G AGE + T H KKLD+E++ Sbjct: 367 TRGGHDPHKIYAAFKVAQESKDQPTVILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIRA 426 Query: 436 FRDRFKLPLTDEQCRALAFYKPADDSPEMKHLHARRAALGGYLP-RRQAGQARRAVPPLA 494 RDRF+LP++D + F+KP+DD+PEM++LH RR ALGGYLP RR + VP L+ Sbjct: 427 MRDRFQLPISDADLPNIPFFKPSDDAPEMQYLHERRKALGGYLPQRRVQADEKLPVPELS 486 Query: 495 A-HSKFALEADGKEMSSTMALVRQLGNLLKDKDFGQYVVPIVADEARTFGMANLFRQVGI 553 A + A+G+E+S+T A R L LL+D + G VVPI+ DE+RTFGM LFRQ+GI Sbjct: 487 AFQAMLEPTAEGREISTTAAYSRVLTALLRDPNLGPRVVPIMVDESRTFGMEGLFRQIGI 546 Query: 554 YSSQGQLYEPEDIGSILYYREAKDGQILEEGITEAGAISSWTAAATAYSVHGTPMLPFYI 613 +S GQLYEP D ++YYRE K GQIL+EGI EAG +SSW AAAT+YS + M+PFY Sbjct: 547 FSQVGQLYEPVDKDQVMYYREDKAGQILQEGINEAGGMSSWIAAATSYSTNNRVMIPFYT 606 Query: 614 YYSMFGFQRIGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGNSLVTAASIPNCVS 673 YYSMFG QRIGDL WAA D RARGFLIG T+GRTTL GEGLQH+DG+S V A++IPNC+ Sbjct: 607 YYSMFGLQRIGDLAWAAGDMRARGFLIGGTAGRTTLNGEGLQHEDGHSHVFASAIPNCIP 666 Query: 674 YDPAYAYELAVILDEGMRRMMERGDDVFYYITVTNENEAQPSLPEGVQEGILRGMYCL-- 731 YDP +A+E+AVI+ +G+RRM+ +DVFYYITV NEN P + G +EGI++G+Y L Sbjct: 667 YDPTFAHEVAVIIHDGLRRMVANQEDVFYYITVMNENYGHPGIKPGQEEGIIKGLYLLNE 726 Query: 732 -GRKPGAQVRLLGSGPILKEAVAASALLQQHWQIEAEVWSVTSYTELARDGVAAQRAQRL 790 G++ +V+LLGSG IL+E +AA+ LL+ W+++A+VWS S+T LARDG +R L Sbjct: 727 GGKQNKLRVQLLGSGTILREVIAAADLLRDDWKVDADVWSAPSFTLLARDGQDVERWNML 786 Query: 791 SGSDG--EPYVTAQLRDSAAPVIAVSDYIRTLPESIRAFVPA--PYVTLGTDGFGRSDTR 846 ++ + Y L S P++ +DY+RT E +RAFVP Y LGTDG+GRSDTR Sbjct: 787 HPAEAPKKSYFEQSLEGSEGPIVVSTDYMRTYAEQVRAFVPKGRSYKVLGTDGYGRSDTR 846 Query: 847 VNLRDFFEIDARWIAYTALCEVWGNDRARLQEAARTL---GIDTAKPLSSTV 895 LR+FFE++ ++ AL + + + + A+ + GID KP T+ Sbjct: 847 AKLREFFEVNRYFVTVAALKSLADEGKIKPEVVAQAIAKYGIDPNKPNPVTL 898 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,950,599 Number of extensions: 133466 Number of successful extensions: 423 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 417 Number of HSP's successfully gapped: 2 Length of query: 895 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 801 Effective length of database: 1,174,780 Effective search space: 940998780 Effective search space used: 940998780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)