BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0009.p (663 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 1301 0.0 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 246 3e-66 gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 229 8e-61 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 155 1e-38 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 1301 bits (3368), Expect = 0.0, Method: Compositional matrix adjust. Identities = 662/663 (99%), Positives = 663/663 (100%) Query: 1 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIVIGPHGLGFAEDNETTHALGEF 60 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGI+IGPHGLGFAEDNETTHALGEF Sbjct: 1 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEF 60 Query: 61 GVVFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQ 120 GVVFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQ Sbjct: 61 GVVFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQ 120 Query: 121 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANES 180 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANES Sbjct: 121 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANES 180 Query: 181 GNIMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT 240 GNIMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT Sbjct: 181 GNIMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWIT 240 Query: 241 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWF 300 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWF Sbjct: 241 EKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWF 300 Query: 301 LVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 LVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP Sbjct: 301 LVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 Query: 361 MLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA 420 MLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA Sbjct: 361 MLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVIIA 420 Query: 421 GFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA 480 GFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA Sbjct: 421 GFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHRAA 480 Query: 481 ALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML 540 ALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML Sbjct: 481 ALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSLML 540 Query: 541 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIGGA 600 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIGGA Sbjct: 541 ASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAVTLIGGA 600 Query: 601 SALGHTLAEIDLRAHCGAEVASLRRNKQRIEPPEQTVLMAGDIVVLRGSSESLLRAEKRL 660 SALGHTLAEIDLRAHCGAEVASLRRNKQRIEPPEQTVLMAGDIVVLRGSSESLLRAEKRL Sbjct: 601 SALGHTLAEIDLRAHCGAEVASLRRNKQRIEPPEQTVLMAGDIVVLRGSSESLLRAEKRL 660 Query: 661 LSK 663 LSK Sbjct: 661 LSK 663 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 246 bits (629), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 184/532 (34%), Positives = 278/532 (52%), Gaps = 30/532 (5%) Query: 11 LLAAAVLGVVAFRMMQLPPMLGYLVVGIVIGPHGLGFAEDNETTHALGEFGVVFLMFSIG 70 L+ A + G +A R+ ++PP++GYLV GI IGP GF D + L E GV+ LMF +G Sbjct: 15 LVLAFIFGTLAHRL-KMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVG 73 Query: 71 LEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAA--FALGGA 128 L FSL L ++++I VAQ+ M LGW + W F G A Sbjct: 74 LHFSLKDLLSVKNIAIPGAVAQITIATLLGMALGW----------LLGWPVGEGFLFGLA 123 Query: 129 LTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANE--------S 180 L+++ST ++ K L ER +ET+ GR +G L+ +D+A+V L+++PAL+ S Sbjct: 124 LSVASTVVLLKALQERRLVETQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALS 183 Query: 181 GN-IMVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWI 239 GN +++TLG GK + A++L G +++ + S+ELF L +L I LG A Sbjct: 184 GNAVLLTLGITLGKVVAFAAVMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALG 243 Query: 240 TEKA-GLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDH 298 + G+S ALGAF AGM++SE+E H+ E+ RD LFF+++GML + V Sbjct: 244 SAYVFGVSFALGAFFAGMILSESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVARE 303 Query: 299 WFLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLV 358 LVL + V+ K + R FG G AL + LAQ GEF F+L + L L+ Sbjct: 304 PLLVLATVLIIVVGKSLGALLIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLL 363 Query: 359 DPMLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKHVI 418 PM +IL +LS+L P + + D ++ A + + + +Q HV+ Sbjct: 364 SPMARDLILGGAILSILLNPLLFSLLD----RYEARQ---PKAPQGGDEPQKVELQDHVV 416 Query: 419 IAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHR 478 + GFGR G+ + L +G + +E E ++ R G YG+AA+ E L AA I R Sbjct: 417 LVGFGRVGRGIGEQLRAQGKPFVVIEAQLENLEALRAAGVPALYGNAAQSELLQAAAIDR 476 Query: 479 AAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVV 530 A L+V A +V+ +A EL P L V+ R+H DA++E L GA V+ Sbjct: 477 ARWLLVAIPEVFEAGQVIENALELNPGLQVVARAHSDAEIEHLEKHGAQRVI 528 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 229 bits (583), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 202/645 (31%), Positives = 308/645 (47%), Gaps = 49/645 (7%) Query: 1 MFSGLELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIVIGPHGLGFAEDNETTHALGEF 60 M L + L L AAV V + + L +LGYL+ GIVIGP GLG + + EF Sbjct: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 Query: 61 GVVFLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQ 120 GVV L+F IGLE +L A+R +FG G QVA A + ++ W+ Sbjct: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAA--------LAASVPWR 112 Query: 121 AAFALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----AL 176 +++SSTAI L ER T G +LLFQD+A +P++ VP A Sbjct: 113 IGLIGALGMSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAA 172 Query: 177 ANESGNIMVTLGW-AAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLG 235 + SG GW AG+ +V+ALL+F G LMR RI+ K +E+F LL+ + Sbjct: 173 SQGSGQ-----GWLHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIA 227 Query: 236 AAWITEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTV 295 + + G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ Sbjct: 228 TCLLMQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVF 287 Query: 296 IDHWFLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGL 355 + +L ++ G + +K ++ AL R FG G L L+Q GEF F++ A Sbjct: 288 FAQPWRILGMVLGFLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTA 347 Query: 356 KLVDPMLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQK 415 K++ P +++ + LSML TP +L D ++++ WM + + Q Sbjct: 348 KVLTPETSSLLVVVVALSMLVTPLLLLVHDRLLVR-----WMEGG---KRRPEDKIDPQD 399 Query: 416 H-VIIAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAA 474 H VIIAGFGR GQ + RLL GI L+ DP+ + R G V YGDA R + L A Sbjct: 400 HAVIIAGFGRFGQIIGRLLAANGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTA 459 Query: 475 GIHRAAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEAL 534 G +A LV+ +AL+++ E P L + R+ + L+ G T + E Sbjct: 460 GADKARVLVIAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETF 519 Query: 535 EGSLMLASHALVMLGVPLRRVVHRVQDARDERYASLRGYFHGMSDGEADGVGMNVRLQAV 594 E +L + L L R R +L + + +A V M V+ Sbjct: 520 ESALHMGRQVLQQLDFGAYRAREAAMKFRAHNLKTLEALYPHYKN-QAQMVSMAVQ---- 574 Query: 595 TLIGGASALGHTLAEIDLRAHCGAEVASLRR----NKQRIEPPEQ 635 A +L A + ++ R ++ R EPP Q Sbjct: 575 -------------AREELEAMFAGDRQAMEREHSHDQWRGEPPPQ 606 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 155 bits (391), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 110/390 (28%), Positives = 202/390 (51%), Gaps = 17/390 (4%) Query: 6 ELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIVIGPHGLGF--AEDNETTHALGEFGVV 63 ++ + +L A ++ VV F ++ P +LGY+V G++IGPH + +D +T H L E GV+ Sbjct: 10 DMAIIMLIAGIVTVV-FNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVI 68 Query: 64 FLMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAF 123 FL+FS+GLEFSL KL+ + F A++ I +G +F A Sbjct: 69 FLLFSLGLEFSLKKLAKVGATAFIGAAAEILLMIWIGYEIG--------LYFGWKRMDAI 120 Query: 124 ALGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVPALANESGNI 183 LG L +SST I+ K L E + + I G+L+ +D+ + ++ ++ +A Sbjct: 121 FLGAMLAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVD 180 Query: 184 MVTLGWAAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWITEKA 243 + GK ++ + + L G + + + +S E+ ++ +L + G + K Sbjct: 181 STEVFTTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKL 240 Query: 244 GLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHWFLVL 303 S+ALGAF+ G +++E+ H++E ++ RD+ +FF+ IG+L + ++ +W + Sbjct: 241 QYSVALGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYWMPIT 300 Query: 304 LLLTGPV---LLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVDP 360 ++ V L+ GL +A G TP +RVG+GLAQ GEF F++ + LK+ Sbjct: 301 VITLAVVFGKLISCGLGTFIAGQGGRTP---MRVGMGLAQIGEFSFIIAALGQSLKVTSD 357 Query: 361 MLIQVILAAMVLSMLATPFILAKSDAIVMK 390 L +++A ++ L TP+++ +D + K Sbjct: 358 FLYPIVVAVSAVTALLTPYLIKAADPLSAK 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,782,173 Number of extensions: 71699 Number of successful extensions: 457 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 445 Number of HSP's successfully gapped: 5 Length of query: 663 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 572 Effective length of database: 1,188,985 Effective search space: 680099420 Effective search space used: 680099420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)