BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0236.p (761 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 1432 0.0 gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPas... 258 1e-69 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 218 2e-57 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 201 2e-52 gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 102 1e-22 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 1432 bits (3708), Expect = 0.0, Method: Compositional matrix adjust. Identities = 725/734 (98%), Positives = 730/734 (99%) Query: 28 MGLQALAQWAWLAGPLVVLMAVLADTAMAMRRGNMKLDLIALVSIAGAVLLREDLVAAVI 87 MGLQALAQWAWLAGPLVVLMAVLADTAMAMRRG MKLDLIALVSIAGAVLLREDLVAAVI Sbjct: 1 MGLQALAQWAWLAGPLVVLMAVLADTAMAMRRGGMKLDLIALVSIAGAVLLREDLVAAVI 60 Query: 88 ALMFAGGRALEGVAQRRAQEEMSALLGKAPHVANRYEGERIVTIGLNLVRPGDRLLVRPG 147 ALMFAGGRALE VAQRRAQ+EMSALLGKAPHVANRYEGERIVTIGLNLVRPGDRLLVRPG Sbjct: 61 ALMFAGGRALESVAQRRAQQEMSALLGKAPHVANRYEGERIVTIGLNLVRPGDRLLVRPG 120 Query: 148 EPVPVDGRLLGPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFA 207 EPVPVDGRLLGPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFA Sbjct: 121 EPVPVDGRLLGPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFA 180 Query: 208 QVLRLVQAAQASQAPAARMADRYALLFIPLVLAMAALSWLLTGDPRRALAVVVVATPCPL 267 QVLRLVQAAQASQAPAARMADRYALLFIPLVLAMAALSWLLTGDPRRALAVVVVATPCPL Sbjct: 181 QVLRLVQAAQASQAPAARMADRYALLFIPLVLAMAALSWLLTGDPRRALAVVVVATPCPL 240 Query: 268 ILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTPS 327 ILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTPS Sbjct: 241 ILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTPS 300 Query: 328 IQANELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSGRVDGKLVR 387 IQA+ELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSGRVDGKLVR Sbjct: 301 IQADELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSGRVDGKLVR 360 Query: 388 VGAMQYVIGPNAAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRLDTPRALRQ 447 VGAMQYVIGP+AAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRLDTPRALRQ Sbjct: 361 VGAMQYVIGPSAAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRLDTPRALRQ 420 Query: 448 LRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREGVTMMVGDG 507 LRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREGVTMMVGDG Sbjct: 421 LRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREGVTMMVGDG 480 Query: 508 INDAPALAAPDIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARKIALQSVVA 567 INDAPALAA DIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARKIALQSVVA Sbjct: 481 INDAPALAAADIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARKIALQSVVA 540 Query: 568 GMGLSGCAMLAAAFGYLPPVAGAILQEFIDVAVILNALRVLYVERGNLSDRLSAEDCQTL 627 GMGLSGCAMLAAAFGYLPPVAGAILQEFIDVAVILNALRVLYVERGNLSDRLSAEDCQTL Sbjct: 541 GMGLSGCAMLAAAFGYLPPVAGAILQEFIDVAVILNALRVLYVERGNLSDRLSAEDCQTL 600 Query: 628 REEHVRLQPIIDRLVWLADRVEDLSSAAMREELVLLSAQLQGNLLLHEAQDDGVLYPRLA 687 REEHVRLQPIIDRLVWLADRVEDLSSAAMREELVLLSAQLQGNLL HEAQDDGVLYPRLA Sbjct: 601 REEHVRLQPIIDRLVWLADRVEDLSSAAMREELVLLSAQLQGNLLQHEAQDDGVLYPRLA 660 Query: 688 QLIGGEDPMGAMSSMHREIYRLSRSVDRLATQAPEDEQQDDAFMLEVRRTLYALHAVLRL 747 QLIGGEDPMGAMSSMHREIYRLSR+VDRLATQAP+DEQQDDAFMLEVRRTLYALHAVLRL Sbjct: 661 QLIGGEDPMGAMSSMHREIYRLSRTVDRLATQAPDDEQQDDAFMLEVRRTLYALHAVLRL 720 Query: 748 HFAQEEEIFHGLSA 761 HFAQEEEIFHGLSA Sbjct: 721 HFAQEEEIFHGLSA 734 >gi|300310933|ref|YP_003775025.1| metal-transporting P-type ATPase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 778 Score = 258 bits (659), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 186/556 (33%), Positives = 306/556 (55%), Gaps = 33/556 (5%) Query: 39 LAGPLVVLMAVLADTAMAMRRGNMKLDLIALVSIAGAVLLREDLVAAVIALMFAGGRALE 98 LA L+ + L +A+R ++ ++ + +++ GA ++ + AAV+ ++F +E Sbjct: 188 LAAILLGGLGTLKKGWIALRNFSLNMNFLMSLAVIGAAVIGQWPEAAVVIVLFTLAEMIE 247 Query: 99 GVAQRRAQEEMSALLGKAPHVANRYEGERIV--TIGLNLVRPGDRLLVRPGEPVPVDGRL 156 ++ RA+ ++ LL P +A R GE V T+ V D + V PGE VP+DG + Sbjct: 248 ALSLDRARNAIAGLLAMTPDMATR-RGEDGVWSTVPARTVTVDDTVRVAPGERVPLDGLV 306 Query: 157 L-GPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFAQVLRLVQA 215 L G ++++ +TGES+PV G + +G +N AF++ T+ ST A++++ VQ Sbjct: 307 LSGSTSINQAPITGESIPVQKQAGDPVFAGTINEQGAFEMRVTAAQGDSTLARIVKSVQQ 366 Query: 216 AQASQAPAARMADRYALLFIPLVLAM--------------AALSWLLTGDPRRALAVVVV 261 AQ +AP R D +A +IP V+ M A WL +AL ++V+ Sbjct: 367 AQGQRAPTQRFVDNFARYYIPAVVVMAVLVALLPPLLAGAAFQPWLY-----KALVLLVI 421 Query: 262 ATPCPLILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMN 321 A PC L+++ PV IV ++ A+ G+L+K G LE+ Q+R L DKTGTLT GK R+ + Sbjct: 422 ACPCALVISTPVTIVSGLASAARHGILVKGGVYLEQGRQLRALALDKTGTLTFGKPRVTD 481 Query: 322 MVTTPSIQANE--LLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSG 379 +V+ + QA++ LL+LA SL S+H +++A+ AA E + + + G G+ G Sbjct: 482 VVSLQA-QADQQALLQLAVSLAARSDHPVSRAI--AAQEEIATVEV-SEFAALAGRGVQG 537 Query: 380 RVDGKLVRVGAMQYVIGPNAAPPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRL 439 R++G+L+ +G + V P + +G + +DGQ ++ +AD +R Sbjct: 538 RINGQLLHLGNHRLVHELGQCSPELEARLQAFEKQGKTTTVLCLDGQPQLLVAVADTVRP 597 Query: 440 DTPRALRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQAS-RE 498 + +A+ +LRALG + ++MLTGD A I + G D +Q P KL AI++ S R Sbjct: 598 SSRQAVAELRALGVE-VIMLTGDNSHTAQAIAAQTGISDARGDQLPEDKLKAIEELSQRH 656 Query: 499 GV--TMMVGDGINDAPALAAPDIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQ 556 GV MVGDGINDAPALA DIG AMGA G A E+A V L+ D L ++ +R++++ Sbjct: 657 GVRGVGMVGDGINDAPALARADIGFAMGAAGTDTALETADVALMDDDLRKIPDFIRLSRK 716 Query: 557 ARKIALQSVVAGMGLS 572 A + +Q++ +G+ Sbjct: 717 AHAVLVQNISIALGIK 732 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 218 bits (554), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 189/539 (35%), Positives = 294/539 (54%), Gaps = 21/539 (3%) Query: 84 AAVIALMFAGGRALEGVAQRRAQEEMSALLGKAPHVAN-RYEGERIVTIGLNLVRPGDRL 142 +AV+ + G+ LEG A+R+ + ++AL P A R +G+ I + L VR GD + Sbjct: 195 SAVVITLVLLGKWLEGRAKRQTADAIAALNALRPENARVRRDGQDI-EVPLAQVRVGDLV 253 Query: 143 LVRPGEPVPVDGRLL-GPAVVDESMLTGESLPVSHPQGATLASGCVNAGNAFDLLATSTD 201 ++RPGE VPVDG + G + +DE+MLTGES P + G L +G +NA + T+ Sbjct: 254 VLRPGERVPVDGVVREGRSHLDEAMLTGESRPQAKQAGDPLTAGSINAEGVLVMETTAIG 313 Query: 202 QSSTFAQVLRLVQAAQASQAPAARMADRYALLFIPLVLAMAALSWL----LTGDPRRAL- 256 + A+++R+V+ AQA++AP R+ D+ + +F+P VL ++ L+ L + G+ +AL Sbjct: 314 AETVLARIVRMVEQAQAAKAPIQRLVDQVSAVFVPAVLLLSLLTLLGWGLVQGNWEQALL 373 Query: 257 ---AVVVVATPCPLILAVPVAIVCAMSRCAQRGVLIKHGGALEKLAQVRTLFFDKTGTLT 313 AV+V+A PC L LA P AI+ A+ G+LIK ALE +V + FDKTGTLT Sbjct: 374 NAVAVMVIACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLT 433 Query: 314 AGKARLMNMVTTPSIQANELLRLAASLEQMSNHVIAQAVVSAAIERALVLSIPDQVQEQP 373 G RL+ + TPS Q +ELL LAA+L+ S H + +AV AA + A +QV+ Sbjct: 434 LGHPRLLELEATPSTQPDELLALAAALQAHSEHPLGRAVREAAAQSAQAARQAEQVRSVA 493 Query: 374 GAGLSGRVDGKLVRVGAMQYVIGPNAAPPWTSGLRDRLKYEGGAGVCVAVDG----QLCG 429 G G+ G V+G+ + +G+ +++ A P ++L+ +G +A ++ G Sbjct: 494 GLGIEGVVEGRQLMLGSSRWMQQLQAHAPALQARAEQLQAQGRTLSWLARRNDSGVEVLG 553 Query: 430 VLYLADDIRLDTPRALRQLRALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKL 489 +L D I+ A+ L +G R ML+GD A + S LG +V E PA K Sbjct: 554 LLAFGDQIKPGAQAAIATLHRMGV-RSAMLSGDNLGAAQQVASELGIQEVRAEVLPADKA 612 Query: 490 AAIKQASREG---VTMMVGDGINDAPALAAPDIGVAMGARGAAAAAESAQVVLLVDRLDR 546 A+ A R+G V MVGDG+NDAPALAA D+G+AM + G A ++A V L+ Sbjct: 613 EAVT-ALRQGGAQVVAMVGDGVNDAPALAAADVGMAM-STGTDVAMQAAGVTLMHGDPGL 670 Query: 547 LAFAVRMAQQARKIALQSVVAGMGLSGCAMLAAAFGYLPPVAGAILQEFIDVAVILNAL 605 +A A+ ++++ Q++ + + AA G L P+ F V+V+ NAL Sbjct: 671 VADAIDISRRTYAKIRQNLFWAFIYNLVGIPLAALGLLNPMLAGAAMAFSSVSVVSNAL 729 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 201 bits (512), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 179/509 (35%), Positives = 255/509 (50%), Gaps = 25/509 (4%) Query: 110 SALLGKAPHVANRYEGERIVTIGLNLVRPGDRLLVRPGEPVPVDGRLL-GPAVVDESMLT 168 SALL AP + E +V L R GD +L RPG+ + D LL G D ++L+ Sbjct: 321 SALL--APGYPEQRETTLVVAAQL---REGDVILARPGDTIAADACLLEGAGEFDLALLS 375 Query: 169 GESLPVSHPQGATLASGCVNAGNAFDLLATSTDQSSTFAQVLRLVQAAQASQAPAARMAD 228 GES P S G G VN L ST A + RL + A + A+ AD Sbjct: 376 GESRPQSFQIGQEAPGGAVNLSQPVFLRVVRATGESTLAALTRLAEQAGQGKPALAQWAD 435 Query: 229 RYALLFIPLVLAMAA---LSWLLTGDPRRA----LAVVVVATPCPLILAVPVAIVCAMSR 281 A F+ +L +A L+W L DP RA +AV+VV+ PC L LA P A+ A R Sbjct: 436 VVASRFVVALLLLAGATFLAWQLI-DPTRAWSTAIAVLVVSCPCALSLATPSALAAATDR 494 Query: 282 CAQRGVLIKHGGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTPSIQANELLRLAASLE 341 + G LI G LE L + T+ FDKTGTLT G+ +L + + + L +AA++E Sbjct: 495 LLRAGTLIVRGNVLETLQRADTIVFDKTGTLTQGRPQLTALWS--DVDCQRSLAIAAAME 552 Query: 342 QMSNHVIAQAVVSAAIERALVLSIPDQVQEQPGAGLSGRVDGKLVRVGAMQYVIG--PNA 399 + S H +A+A+V R + +Q+ GAG+ G ++G L R+G+ +V G A Sbjct: 553 RGSLHPLAKALVEEGARRQVDALEVEQLTSVTGAGMQGMIEGDLYRIGSRSFVAGLAGTA 612 Query: 400 APPWTSGLRDRLKYEGGAGVCVAVDGQLCGVLYLADDIRLDTPRALRQLRALGAKRIVML 459 PP L G+ + DG L LAD +R D + RALG R ++L Sbjct: 613 LPP---VLLQSAASGAGSVYLGSSDGWL-ARFDLADALRTDAVATVAAFRALG-LRTILL 667 Query: 460 TGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREG-VTMMVGDGINDAPALAAPD 518 +GD+ +V + ++ G +V +P KL ++ R+G V +VGDGINDA L A D Sbjct: 668 SGDQPEVCAEVAAAAGISEVQAACTPQGKLDYVRNLQRDGAVVAIVGDGINDAAMLRAGD 727 Query: 519 IGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQARKIALQSVVAGMGLSGCAMLA 578 + AMG +GAA A SA V++ DRL +A MAQ+ I Q++V + A+ A Sbjct: 728 VSFAMG-KGAALAQVSADAVIMSDRLQAVADCAAMAQRTMHIVRQNLVWAATYNFLAIPA 786 Query: 579 AAFGYLPPVAGAILQEFIDVAVILNALRV 607 AAFG L P A+ + V+ NALR+ Sbjct: 787 AAFGLLDPWMSAVGMSLSSLLVVGNALRL 815 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 102 bits (254), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 142/528 (26%), Positives = 226/528 (42%), Gaps = 85/528 (16%) Query: 98 EGVAQRRAQEEMSALLG-----------KAPHVANRYEGERIVTIGLNLV-----RPGDR 141 E +A+ R++ + +AL G +A HV R +G +++ +V R GD Sbjct: 104 EALAEGRSKAQAAALRGVKKSVMAKKLRRADHV--RGDGMQVLASQCEVVEGDSLRKGDL 161 Query: 142 LLVRPGEPVPVDGRLL-GPAVVDESMLTGESLPVSHPQGATLA--SGCVNAGNAFDLLAT 198 +L+ G+ VP D ++ G A VDES +TGES PV G + +G + + ++ Sbjct: 162 VLIEAGDTVPADAEVIEGVATVDESAITGESAPVIRESGGDFSAVTGGTRVLSDWIVVRV 221 Query: 199 STDQSSTF-AQVLRLVQAAQASQAP-----------AARMADRYALLFIPLVLAMAALSW 246 S + F +++ +V+ A+ + P + + +P ++ +++ Sbjct: 222 SVNPGEAFLDRMIAMVEGARRQKTPNELALTLLLVALTLVFLLVTVTLLPF--SIFSVNA 279 Query: 247 LLTGDPRRALAVVVVATPCPLILAVPVAI--------VCAMSRCAQRGVLIKHGGALEKL 298 +G P V V A L+ +P I V MSR Q V+ G A+E Sbjct: 280 AGSGSP-----VSVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAA 334 Query: 299 AQVRTLFFDKTGTLTAGKARLMNMVTTPSIQANELLRLA--ASLEQMSNHVIAQAVVSAA 356 V L DKTGT+T G + + P + EL +A ASL + +++V A Sbjct: 335 GDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELADVAQLASLADETPE--GRSIVVLA 392 Query: 357 IERALVLSIPDQVQEQPGAGL-----SGRVDG-----KLVRVGAMQYVIGPNAA-----P 401 R ++ ++ GA RV G + +R GA + A P Sbjct: 393 KNR---FNLREREMANLGAEFIPFSAQTRVSGIDLGHRRIRKGAADAIRKLTRAADQRLP 449 Query: 402 PWTSGLRDRLKYEGGAGVCV-------AVDGQ-----LCGVLYLADDIRLDTPRALRQLR 449 + + GG + V A+ Q + G + L D ++ +LR Sbjct: 450 AQVESDIESIARRGGTPLVVIEWHAEAAMINQGAPIRILGTVELKDIVKGGIKERFGELR 509 Query: 450 ALGAKRIVMLTGDRRDVAMTIGSSLGFDDVMPEQSPASKLAAIKQASREG-VTMMVGDGI 508 +G K VM+TGD R A I + G DD + E +P KLA I+Q EG + M GDG Sbjct: 510 RMGIK-TVMITGDNRLTAAAIAAEAGVDDFLAEATPEQKLALIRQHQAEGKLVAMTGDGT 568 Query: 509 NDAPALAAPDIGVAMGARGAAAAAESAQVVLLVDRLDRLAFAVRMAQQ 556 NDAPALA D+ VAM + G AA E+ +V L +L V + +Q Sbjct: 569 NDAPALAQADVAVAMNS-GTQAAKEAGNMVDLDSNPTKLIEIVEIGKQ 615 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,159,157 Number of extensions: 88983 Number of successful extensions: 449 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 426 Number of HSP's successfully gapped: 9 Length of query: 761 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 668 Effective length of database: 1,179,515 Effective search space: 787916020 Effective search space used: 787916020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 134 (56.2 bits)