BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0220.p (579 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synt... 1177 0.0 gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthe... 325 5e-90 >gi|300311720|ref|YP_003775812.1| poly-beta-hydroxyalkanoate synthase [Herbaspirillum seropedicae SmR1] Length = 590 Score = 1177 bits (3044), Expect = 0.0, Method: Compositional matrix adjust. Identities = 576/579 (99%), Positives = 576/579 (99%) Query: 1 VAGLQEAAIPVAPTVRLDRQIHAALARASGSLSLVSGLLAVTDWAAHLAVSPGKRLELLC 60 VAGLQEAAIPVAPTVRLDRQIHAALARASGSLSLVSGLLAVTDWAAHLAVSPGKRLELLC Sbjct: 12 VAGLQEAAIPVAPTVRLDRQIHAALARASGSLSLVSGLLAVTDWAAHLAVSPGKRLELLC 71 Query: 61 LALQQANHLLHYISRSSSSDPDSPSMLPVADRRFSNPDWQRWPFNLWHQSFLLAEQWWAA 120 LALQQANHLLHYISRSSSSDPDSPSMLPVADRRFSNPDWQRWPFNLWHQSFLLAEQWWAA Sbjct: 72 LALQQANHLLHYISRSSSSDPDSPSMLPVADRRFSNPDWQRWPFNLWHQSFLLAEQWWAA 131 Query: 121 ATRDVWGVEKHHADLVAFAARQCLDVFSPSNQLLTNPEVLRITMEQRGANLLMGMQNCMN 180 ATRDVWGVEKHHADLVAFAARQCLDVFSPSNQLLTNPEVLRITMEQRGANLLMGMQNCMN Sbjct: 132 ATRDVWGVEKHHADLVAFAARQCLDVFSPSNQLLTNPEVLRITMEQRGANLLMGMQNCMN 191 Query: 181 DMIGLLLEKPASAARTFMPGRDVAVSPGKVVLRNRIMELIRYAPATETVYPEPLLLIPAW 240 DMIGLLLEKPAS ARTFMPGRDVAVSPGKVVLRNRIMELIRYAPATETV PEPLLLIPAW Sbjct: 192 DMIGLLLEKPASTARTFMPGRDVAVSPGKVVLRNRIMELIRYAPATETVCPEPLLLIPAW 251 Query: 241 IMKYYILDLSPANSLIRYLVEKGHTVFCVSWKNPAPADRDLSMDDYLDSGFHAALDAVNS 300 IMKYYILDLSPANSLIRYLVEKGHTVFCVSWKNPAPADRDLSMDDYLDSGFHAALDAVNS Sbjct: 252 IMKYYILDLSPANSLIRYLVEKGHTVFCVSWKNPAPADRDLSMDDYLDSGFHAALDAVNS 311 Query: 301 ACPDRKVHGVGYCLGGTLLSIAAAAMARDGDQRLASLTLLAAQTDFTEPGELGLFIDESQ 360 ACPDRKVHGVGYCLGGTLLSIAAAAMARDGDQRLASLTLLAAQTDFTEPGELGLFIDESQ Sbjct: 312 ACPDRKVHGVGYCLGGTLLSIAAAAMARDGDQRLASLTLLAAQTDFTEPGELGLFIDESQ 371 Query: 361 ISLLEAQMQQAGTLSQSQMAAAFQMLRSYDLLWSRVIRQYLMGVPAHNSDLMEWNADATR 420 ISLLEAQMQQAGTLSQSQMAAAFQMLRSYDLLWSRVIRQYLMGVPAHNSDLMEWNADATR Sbjct: 372 ISLLEAQMQQAGTLSQSQMAAAFQMLRSYDLLWSRVIRQYLMGVPAHNSDLMEWNADATR 431 Query: 421 MPALMHSQYLRRLFLDDDLSEGRYEVDGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLH 480 MPALMHSQYLRRLFLDDDLSEGRYEVDGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLH Sbjct: 432 MPALMHSQYLRRLFLDDDLSEGRYEVDGKPVLLADVNLPVFLVGTVRDHVAPWRSVYKLH 491 Query: 481 SLCPAEITFVLASGGHNAGIVSPPGHSRSQFQISTRAAGASYRSADEWMAGTSVTTGSWW 540 SLCPAEITFVLASGGHNAGIVSPPGH RSQFQISTRAAGASYRSADEWMAGTSVTTGSWW Sbjct: 492 SLCPAEITFVLASGGHNAGIVSPPGHPRSQFQISTRAAGASYRSADEWMAGTSVTTGSWW 551 Query: 541 PAWADWLAQRSGTPVPAAGMGYDKAHSLADAPGNYIMEQ 579 PAWADWLAQRSGTPVPAAGMGYDKAHSLADAPGNYIMEQ Sbjct: 552 PAWADWLAQRSGTPVPAAGMGYDKAHSLADAPGNYIMEQ 590 >gi|300312303|ref|YP_003776395.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 588 Score = 325 bits (833), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 184/495 (37%), Positives = 273/495 (55%), Gaps = 12/495 (2%) Query: 89 VADRRFSNPDWQRWP-FNLWHQSFLLAEQWWAAATRDVWGVEKHHADLVAFAARQCLDVF 147 + DRR S P WQ P F ++ L ++ A V G EK L FA +Q +D Sbjct: 90 ITDRRLSAPAWQEHPYFAFQAAAYQLNSRFLLALADAVQGSEKVRHKL-RFAVQQMIDAM 148 Query: 148 SPSNQLLTNPEVLRITMEQRGANLLMGMQNCMNDMIGLLLEKPASAARTFMPGRDVAVSP 207 SP+N L TNP + +E RG +L G+ + D+ + + A F GRDV S Sbjct: 149 SPANFLATNPVAQQKILETRGESLTKGIAQLLADLQKGHVSQTDETA--FEVGRDVGASE 206 Query: 208 GKVVLRNRIMELIRYAPATETVYPEPLLLIPAWIMKYYILDLSPANSLIRYLVEKGHTVF 267 G V+ N + +LI+Y P T+TV+ PLL++P I K+YILDL P NSL+RY VE+GHTVF Sbjct: 207 GSVIFENELFQLIQYKPLTKTVHEVPLLIVPPCINKFYILDLQPHNSLVRYTVEQGHTVF 266 Query: 268 CVSWKNPAPADRDLSMDDYLDSGFHAALDAVNSACPDRKVHGVGYCLGGTLLSIAAAAMA 327 +SW+N A + D Y+D ++ V +++ +G+C+GGT+LS A A +A Sbjct: 267 LISWRNADAAVETATWDQYVDDAVVRSIHIVQEISKQPQINALGFCVGGTILSCALALLA 326 Query: 328 RDGDQRLASLTLLAAQTDFTEPGELGLFIDESQISLLEAQMQQAGTLSQSQMAAAFQMLR 387 G+ +ASLTLL DF++ G + ++IDE Q++L E + Q G ++ A+AF LR Sbjct: 327 ARGEDPVASLTLLTTFLDFSDTGAIDVYIDEMQLALREQMIGQRGLMAGRDFASAFSSLR 386 Query: 388 SYDLLWSRVIRQYLMGVPAHNSDLMEWNADATRMPALMHSQYLRRLFLDDDLSE-GRYEV 446 DLLW+ V YL G DL+ WNAD+T +P M YLR ++L++ L E GR V Sbjct: 387 PNDLLWNYVESNYLKGEKPSAFDLLYWNADSTNLPGPMFCYYLRHMYLENALKEPGRLTV 446 Query: 447 DGKPVLLADVNLPVFLVGTVRDHVAPWRSVYK----LHSLCPAEITFVLASGGHNAGIVS 502 G+ + L +N P F+ + DH+ PW+S Y +++ + FVL + GH AG+++ Sbjct: 447 GGEKIDLRKINAPAFIYASRDDHIVPWKSAYASLGLINTAKRSNNRFVLGASGHIAGVIN 506 Query: 503 PPGHSRSQFQISTRAAGASYRSADEWMAGTSVTTGSWWPAWADWLAQRSGTPVPA-AGMG 561 P ++ + I+ +A S AD W+AG + GSWW WA +LA+ G V A G Sbjct: 507 PASKNKRNYWINDKAKAGS--GADAWLAGATEHPGSWWGQWAAFLAKHGGRKVAAPKAAG 564 Query: 562 YDKAHSLADAPGNYI 576 + + APG Y+ Sbjct: 565 STRYKHIEPAPGRYV 579 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,898,470 Number of extensions: 80725 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 2 Length of query: 579 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 489 Effective length of database: 1,193,720 Effective search space: 583729080 Effective search space used: 583729080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)