BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0218.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311722|ref|YP_003775814.1| phosphate acetyl/butaryl transf... 961 0.0 gi|300309605|ref|YP_003773697.1| phosphate acetyltransferase [He... 311 6e-86 gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [He... 67 4e-12 >gi|300311722|ref|YP_003775814.1| phosphate acetyl/butaryl transferase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 961 bits (2485), Expect = 0.0, Method: Compositional matrix adjust. Identities = 482/484 (99%), Positives = 483/484 (99%) Query: 1 MADSLPAPVVADKDAHASDDVLGLIRNRIFSEIAVGDSATITRTLTLADIQLFAVISGDA 60 MADSLPAPVVADKDAHASDDVLGLIRNRIFSEIAVGDSATITRTLTLADIQLFAVISGDA Sbjct: 1 MADSLPAPVVADKDAHASDDVLGLIRNRIFSEIAVGDSATITRTLTLADIQLFAVISGDA 60 Query: 61 NPQHLDLAFAKETRFHGLIAHGMFGGALISAVLGTRLPGPGTIYLGQTLRFVAPVRIGDT 120 NPQHLDLAFAKETRFHGLIAHGMFGGALISAVLGTRLPGPGTIYLGQTLRFVAPVRIGDT Sbjct: 61 NPQHLDLAFAKETRFHGLIAHGMFGGALISAVLGTRLPGPGTIYLGQTLRFVAPVRIGDT 120 Query: 121 LRTTVTVKERDEARKLLTLACACINQDGVTVIEGEAKVLAPTEPILRRRTSLPEVSMKMG 180 LRTTVTVKERDEARKLLTLACACINQDGVTVIEGEAKVLAPTEPILRRRTSLPEVSMKMG Sbjct: 121 LRTTVTVKERDEARKLLTLACACINQDGVTVIEGEAKVLAPTEPILRRRTSLPEVSMKMG 180 Query: 181 DADLQLLLEHARKPGPAPVAVVHPCDELSLAAVLDAHAAGLISPILVAPRSRLLALAESH 240 DADLQLLLEHARKPGPAPVAVVHPCDELSLAAVLDAHAAGLISPILVAPRSRLLALAESH Sbjct: 181 DADLQLLLEHARKPGPAPVAVVHPCDELSLAAVLDAHAAGLISPILVAPRSRLLALAESH 240 Query: 241 KLDISAFPLEDVPHSHAAAARAIELVMHGKVEMLMKGSLHTEEFMGAVVACPGLHTKRRM 300 KLDISAFPLEDVPHSHAAAARAIELVMHGKVEMLMKGSLHTEEFMGAVVACPGLHTKRRM Sbjct: 241 KLDISAFPLEDVPHSHAAAARAIELVMHGKVEMLMKGSLHTEEFMGAVVACPGLHTKRRM 300 Query: 301 SHCYLMQTPAYPRPFIITDAAVNIEPGLAEKADIIRNAIELAHVIGVKTPKVAILAAVET 360 SHCYLMQTP+YPRPFIITDAAVNIEPGLAEKADIIRNAIELAHVIGVKTPKVAILAAVET Sbjct: 301 SHCYLMQTPSYPRPFIITDAAVNIEPGLAEKADIIRNAIELAHVIGVKTPKVAILAAVET 360 Query: 361 VNPRMPATLDAAALCKMADRGQITGAILDGPLAFDNAVSPVSARVKGISSEVAGQADILV 420 VNPRMPATLDAAALCKMADRGQITGAILDGPLAFDNAVSPVSARVKGI SEVAGQADILV Sbjct: 361 VNPRMPATLDAAALCKMADRGQITGAILDGPLAFDNAVSPVSARVKGIRSEVAGQADILV 420 Query: 421 VPDLESGNLLAKQLEYMGGAISAGLVLGARIPLILTSRADSRETRLASCAVARMLAQHYR 480 VPDLESGNLLAKQLEYMGGAISAGLVLGARIPLILTSRADSRETRLASCAVARMLAQHYR Sbjct: 421 VPDLESGNLLAKQLEYMGGAISAGLVLGARIPLILTSRADSRETRLASCAVARMLAQHYR 480 Query: 481 SAPP 484 SAPP Sbjct: 481 SAPP 484 >gi|300309605|ref|YP_003773697.1| phosphate acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 311 bits (798), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 172/283 (60%), Positives = 212/283 (74%), Gaps = 1/283 (0%) Query: 199 VAVVHPCDELSLAAVLDAHAAGLISPILVAPRSRLLALAESHKLDISAFPLEDVPHSHAA 258 VAVVHPC +L L+A GL P+LV P +R+ +AE+ + + P+ HSHAA Sbjct: 28 VAVVHPCSPEALQGALEARDEGLFDPVLVGPETRIRRIAEAADISLQGLPIVPAEHSHAA 87 Query: 259 AARAIELVMHGKVEMLMKGSLHTEEFMGAVVA-CPGLHTKRRMSHCYLMQTPAYPRPFII 317 AARA+E+ + G+V LMKGSLH++E +GAVV GL T+RR+SH Y M PAYP+P II Sbjct: 88 AARAVEMAVAGEVGALMKGSLHSDELLGAVVGGASGLRTERRISHVYAMAIPAYPKPLII 147 Query: 318 TDAAVNIEPGLAEKADIIRNAIELAHVIGVKTPKVAILAAVETVNPRMPATLDAAALCKM 377 TDAA+NI P LA K DI +NAI+L HV+GV P VA+LAAVETVNP MP+TLDAAAL M Sbjct: 148 TDAAINIAPTLAHKRDICQNAIDLLHVMGVPRPHVAVLAAVETVNPAMPSTLDAAALTVM 207 Query: 378 ADRGQITGAILDGPLAFDNAVSPVSARVKGISSEVAGQADILVVPDLESGNLLAKQLEYM 437 A R QITGA++DGPLAFDNA+S +A VKGI S VAG ADIL+VPDLE+GN+LAKQL Y+ Sbjct: 208 ATRHQITGALVDGPLAFDNAISLQAASVKGIVSPVAGSADILLVPDLEAGNMLAKQLIYL 267 Query: 438 GGAISAGLVLGARIPLILTSRADSRETRLASCAVARMLAQHYR 480 A +AGLVLGAR+P+ILTSRADS RLAS A+AR++A R Sbjct: 268 SQADAAGLVLGARVPIILTSRADSLRVRLASSALARLVASRQR 310 >gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [Herbaspirillum seropedicae SmR1] Length = 686 Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 52/150 (34%), Positives = 74/150 (49%), Gaps = 17/150 (11%) Query: 30 FSEIAVGDSATI-TRTLTLADIQLFAVISGDANPQHLDLAFAKETRFHGLIAHGMFGGAL 88 F ++ +GDS T RT+T ADI F +SGD H D A+++ F IAHG F + Sbjct: 539 FEDLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYF---V 595 Query: 89 ISAVLGTRL-PGPGTI---YLGQTLRFVAPVRIGDTLRTTVTVKER-DEARK-------- 135 +SA G + P PG + Y TLRFV PV IGDT+ +T K + D +K Sbjct: 596 LSAAAGLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQG 655 Query: 136 LLTLACACINQDGVTVIEGEAKVLAPTEPI 165 ++ NQ+G V + L +P+ Sbjct: 656 VVAWDVEVSNQEGELVASYDILTLVAKKPL 685 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,584,738 Number of extensions: 69649 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 3 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)