BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0217.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311724|ref|YP_003775816.1| LysR family transcription regul... 533 e-153 gi|300310792|ref|YP_003774884.1| LysR family transcription regul... 155 3e-39 gi|300311542|ref|YP_003775634.1| LysR family transcription regul... 134 7e-33 gi|300310987|ref|YP_003775079.1| cys regulon transcription regul... 93 2e-20 gi|300312305|ref|YP_003776397.1| LysR family transcription regul... 88 9e-19 >gi|300311724|ref|YP_003775816.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 297 Score = 533 bits (1374), Expect = e-153, Method: Compositional matrix adjust. Identities = 267/269 (99%), Positives = 268/269 (99%) Query: 1 VTQTAVTLRLRKLEDFLGVTLIERTTRSIALTEIGQDFLPQARRLLAELAGALVEIRETG 60 VTQTAVTLRLRKLEDFLGVTLIERTTRSIALTEIG+DFLPQARRLLAELAGALVEIRETG Sbjct: 29 VTQTAVTLRLRKLEDFLGVTLIERTTRSIALTEIGRDFLPQARRLLAELAGALVEIRETG 88 Query: 61 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH 120 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH Sbjct: 89 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH 148 Query: 121 IAGEHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLDP 180 IAGEHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLDP Sbjct: 149 IAGEHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLDP 208 Query: 181 ALKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRS 240 LKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRS Sbjct: 209 TLKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRS 268 Query: 241 LVLITRKAAHLSPAAQALYDMIREHEGDD 269 LVLITRKAAHLSPAAQALYDMIREHEGDD Sbjct: 269 LVLITRKAAHLSPAAQALYDMIREHEGDD 297 >gi|300310792|ref|YP_003774884.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 155 bits (393), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 95/272 (34%), Positives = 150/272 (55%), Gaps = 4/272 (1%) Query: 1 VTQTAVTLRLRKLEDFLGVTLIERTTRSIALTEIGQDFLPQARRLLAELAGALVEIRETG 60 +TQ A++ R+ LE L L +RTTR ++LT G+ FL A+ ++ EL A+ + + Sbjct: 31 ITQPALSRRIENLEQALQERLFDRTTRRVSLTPAGELFLVHAQAVMEELELAVKSVEQRM 90 Query: 61 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH 120 RR V++ACVP+V LP++L+ Y+ + + R++I+D +++ V E+V + E +FG++ Sbjct: 91 AQRREHVTVACVPSVANNLLPQVLKRYARSHANVRVRIIDESAADVLESVRKGEADFGVN 150 Query: 121 IAGEHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLDP 180 G E+ LT +RY+ + P HPLA + W+ L L+ S NR+L+D Sbjct: 151 FIGAQEAEIDFAELTRERYLAVMPTHHPLAAHASLNWKSLAGEKLVSVSSSSGNRSLIDN 210 Query: 181 ALKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRS 240 A YE + A+ LVA G+GVA++P+LAI+ Y + + PV+SR+ Sbjct: 211 AFSRVPQRPVIQYETNHVAGAISLVAAGLGVALLPELAIRNAPYKGIVARPVTSPVLSRA 270 Query: 241 LVLITRKAAHLSPA----AQALYDMIREHEGD 268 + LIT K L PA AQAL D I G Sbjct: 271 MGLITLKGRVLKPAAATLAQALRDAISTRSGK 302 >gi|300311542|ref|YP_003775634.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 335 Score = 134 bits (338), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 83/262 (31%), Positives = 142/262 (54%), Gaps = 1/262 (0%) Query: 1 VTQTAVTLRLRKLEDFLGVTLIERTTRSIALTEIGQDFLPQARRLLAELAGALVEIRETG 60 V+Q+A + ++K+E G L ER TR++ LT G+ F+ AR+L++++ A ++ + Sbjct: 31 VSQSAFSAMIQKIEAAAGARLFERDTRNVTLTPEGELFVQVARQLVSDIEAAFSDMSDYV 90 Query: 61 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH 120 R+G V+IA +P++ +LP +L Y RYP +++ D S + + +V+ + Sbjct: 91 ARRKGRVAIAALPSLAAGWLPPVLADYRQRYPGVTVELFDAISDQCLDLLRHGKVDIALT 150 Query: 121 IAGEHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLDP 180 G + E + L D + L+C KDH LA +RRI QL +I +S R LD Sbjct: 151 APGPNLLEFDTRPLCSDPFYLVCRKDHKLASKRRIKVPQLAGCEMIHLARSTSVRQHLDA 210 Query: 181 ALKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSRS 240 L+ G + + EV+ +T L+ G+GV+VVP+L + + P + I L P + R Sbjct: 211 VLRPGGV-IHTGLEVEHLATVAALIESGLGVSVVPELTLFQFRLPNLVAIPLDAPELKRP 269 Query: 241 LVLITRKAAHLSPAAQALYDMI 262 L+++T K LS AAQ L +++ Sbjct: 270 LLIVTPKERSLSVAAQGLLELV 291 >gi|300310987|ref|YP_003775079.1| cys regulon transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 311 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 73/265 (27%), Positives = 121/265 (45%), Gaps = 5/265 (1%) Query: 2 TQTAVTLRLRKLEDFLGVTLIERTTRSI-ALTEIGQDFLPQARRLLAELAGALVEIRETG 60 +Q V+ ++R+LE+ LGV + ER + + LTE G+ L RLL E +E Sbjct: 29 SQPGVSRQIRELEEELGVEIFERNGKRLTGLTEPGKGILGIVERLLLEAENLRRASKEYA 88 Query: 61 IARRGDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIH 120 + G +SIA T LPR++QA+ +P RI + A +AE V+ + + GI Sbjct: 89 GEQSGTLSIATTHTQARYVLPRVVQAFRDAFPQVRIALQQSAPEHIAEWVISGKADIGIA 148 Query: 121 IAG-EHHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLIFAGEVSSNRALLD 179 G EL+S +V++ PK HPL +++ L ++PLI + R +D Sbjct: 149 TEGLSQFKELVSFPCYTWSHVIVVPKGHPLLGLEQVSLEDLGTWPLITYDVGFTGRGHID 208 Query: 180 PALKANGFALRSFYEVQRSSTAVGLVAQGVGVAVVPKLAIQEGAYPTVRVIELVDPVVSR 239 A + G S V+ G+GV +V +A+ +G + + I + Sbjct: 209 EAFRQAGIEPDIVLTAMDSDVIQQYVSLGMGVGLVASMAVDQGKHQEMVAINASHLFKAN 268 Query: 240 SLVLITRKAAHLSPAAQALYDMIRE 264 L R+ A+L A +D I++ Sbjct: 269 IARLAVRRGAYLRGYA---FDFIQQ 290 >gi|300312305|ref|YP_003776397.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 87.8 bits (216), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 70/268 (26%), Positives = 123/268 (45%), Gaps = 13/268 (4%) Query: 5 AVTLRLRKLEDFLGVTLIERTTRSIALTEIGQDFLPQARRLLAELAGALVEIRETGIARR 64 + + R+R +ED LG L+ER R + T+ G+ AR + ++ E+ + + Sbjct: 35 SASARIRNMEDALGTPLLERGRRGVQTTDAGRALAHHARMMAQQMEQLRDELGQYARGLK 94 Query: 65 GDVSIACVPTVGVQYLPRILQAYSARYPHNRIKILDHASSAVAEAVLRREVEFGIHIAGE 124 G + + C + ++LP +L + AR+PH I++ + S V +AV+ + GI Sbjct: 95 GHIRLLCNTSAMTEFLPEVLARFLARHPHTDIELEEKLSYEVVQAVIDGVADVGIIADST 154 Query: 125 HHPELISVALTEDRYVLICPKDHPLARRRRIAWRQLQSYPLI-FAGEVSSNRALLDPALK 183 L + +D V + K HPLARR+ +A+ +L + +G+ + + L A + Sbjct: 155 DSGPLQTYPFRQDHLVAVLSKRHPLARRKSLAFAELLGEDFVGLSGDSALQQHLSGHATR 214 Query: 184 ANG-----FALRSFYEVQRSSTAVGLVAQGVGVAVVPKL-AIQEGAYPTVRVIELVDPVV 237 A LRSF + R +V VGVAV+P+ A+ + +R I L D Sbjct: 215 AGKRLQYRVRLRSFDGICR------MVEHQVGVAVLPETAALASTPHMDIRAIPLSDGWS 268 Query: 238 SRSLVLITRKAAHLSPAAQALYDMIREH 265 R L++ R L + L ++ H Sbjct: 269 RRQLLICVRGLEGLPGPTRELIQALQAH 296 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 697,640 Number of extensions: 24350 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 39 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)