BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0216.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogen... 310 2e-85 gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 116 3e-27 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 115 8e-27 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 107 2e-24 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 102 8e-23 >gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 328 Score = 310 bits (794), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 173/331 (52%), Positives = 220/331 (66%), Gaps = 13/331 (3%) Query: 1 MNITILDDYFDTLRSLPCFRKLDDHTVTIWNDHVQEVDALAERLRDTEVLVLIRERTQIR 60 M I ILDDY D +R L CFR LD H V ++N+ ++ V LA RL + +VLIRERT + Sbjct: 1 MKIAILDDYQDAVRHLDCFRLLDGHDVKVFNNTIRGVGQLAARLSPFDAVVLIRERTALT 60 Query: 61 APLIARLPALRLISQRSAFP-HIDIAACSTHGVIVSSNQHADTPSYAAAELTWGLILAAM 119 PL+ +LP L+L+SQ H+D+AAC+ G+ + T A AELTW LI+AAM Sbjct: 61 RPLLEKLPLLKLVSQTGKVSGHVDVAACTERGIAIVEGVGDPT---APAELTWALIMAAM 117 Query: 120 RQIPQQMRALQDGFWQIG--------VGKTLRGRTLGIYGYGRIGAEVAAYGAAFGMKVL 171 R++PQ AL+ G WQ +G L+GRTLGI+GYG+IG VA YG AFGM+VL Sbjct: 118 RRVPQYCSALRAGAWQQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQVL 177 Query: 172 IWASETSLMRARDDGWDIAPDKKTFFETCDVLSLHMRLVPATRGIVTAHDLGRMKPSALL 231 +W ++S +A DG+ A K+ FF DVLSLH+RL ATRGIV A DL RMK +A Sbjct: 178 VWGRDSSREQAIKDGYQAAASKEEFFAQADVLSLHLRLNDATRGIVKASDLARMKATACF 237 Query: 232 VNTSRAGLIESGALEQALCAGRPGMAAVDVYEKEPLRDTQHPLLQMKNVVCTPHIGYVTE 291 VNTSRA L+E ALE AL AGRPG+AA+DVYE+EPL + LLQM NVV PH+GYV + Sbjct: 238 VNTSRAELVEPDALEAALIAGRPGLAALDVYEREPL-PVESALLQMDNVVAAPHLGYVEK 296 Query: 292 DEFELQFADIFEQIVSFAAGQPIHVVNPEVL 322 D +EL F F+ IV++AAG P HV+NPE L Sbjct: 297 DGYELYFRAAFQNIVAYAAGAPTHVLNPEAL 327 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 116 bits (291), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 89/264 (33%), Positives = 129/264 (48%), Gaps = 27/264 (10%) Query: 70 LRLISQRS-AFPHIDIAACSTHGVIVSSNQHADTPSYAAAELTWGLILAAMRQIPQ-QMR 127 +R ++ RS F HID+AA G+ V+ + AD Y+ AE GL+ A R+I + MR Sbjct: 69 VRFVTTRSTGFNHIDVAAARALGLTVA--RVADYSPYSVAEFAVGLLQAVNRKIARASMR 126 Query: 128 ALQDGFWQIGV-GKTLRGRTLGIYGYGRIGAEVAAYGAAFGMKVLIWASETSLMRARDDG 186 + F G+ G L G+T+G+ G G+IG A A FG ++L S+ R+ Sbjct: 127 TREGNFELDGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQIL--GSDVH----RNPD 180 Query: 187 WDIAP----DKKTFFETCDVLSLHMRLVPATRGIVTAHDLGRMKPSALLVNTSRAGLIES 242 +D ++ F DV+SLH L ATR +V A L KP +LVNTSR GL+++ Sbjct: 181 FDALGGRYVERTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDT 240 Query: 243 GALEQALCAGRPGMAAVDVYEKEP------------LRDTQHPLLQMKNVVCTPHIGYVT 290 A AL +GR A+DVYE+E D L+ NV+ T H + T Sbjct: 241 EAAIAALKSGRLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFT 300 Query: 291 EDEFELQFADIFEQIVSFAAGQPI 314 E+ E + +FAAG + Sbjct: 301 EEAIGQIMQTTIENLSAFAAGDDL 324 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 115 bits (287), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 88/230 (38%), Positives = 122/230 (53%), Gaps = 10/230 (4%) Query: 68 PALRLISQR-SAFPHIDIAACSTHGVIVSSNQHADTPSYAAAELTWGLILAAMRQIPQQM 126 P LR+IS+ + ID A G+ V + A + A AE TW LI+A + + Sbjct: 69 PILRVISKHGTGIDSIDSQAAQQRGIAVKAA--AGANAPAVAEHTWALIMACAKNVVGLD 126 Query: 127 RALQDGFWQIGVGKTL--RGRTLGIYGYGRIGAEVAAYGAAFGMKVLIWASETSLMRARD 184 + +++G W K+L +GRTLG+ G G IG VAA AA GM VL + + Sbjct: 127 QRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVL---AHDPYAKEAP 183 Query: 185 DGWDIAPDKKTFFETCDVLSLHMRLVPATRGIVTAHDLGRMKPSALLVNTSRAGLIESGA 244 G + D T F DV+SLH L + ++ A L RMK A+LVNT+R GLI+ A Sbjct: 184 QGVQLV-DLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQA 242 Query: 245 LEQALCAGRPGMAAVDVYEKEPLRDTQHPLLQMKNVVCTPHIGYVTEDEF 294 L AL +G+ A +D +EKEP HPL ++ N V +PHIG VT D + Sbjct: 243 LIAALDSGKLRAAGLDSFEKEPFT-APHPLQRVGNAVLSPHIGGVTSDAY 291 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 83/274 (30%), Positives = 132/274 (48%), Gaps = 16/274 (5%) Query: 59 IRAPLIARLPALRLISQRS-AFPHIDIAACSTHGVIVSSNQHADTPSYAAAELTWGLILA 117 + A L A P L+ + + + +ID+AA + GV+ ++ D + A+ W L++A Sbjct: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTP--DVLNETTADFGWALMMA 114 Query: 118 AMRQIPQQMRALQDGFWQ-----IGVGKTLRGRTLGIYGYGRIGAEVAAYGAAFGMKVLI 172 A R+I + L+ G W+ VG + G TLGI G GRIG +A F M+VL Sbjct: 115 AARRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVL- 173 Query: 173 WASETSL---MRARDDGWDIAPDKKTFFETCDVLSLHMRLVPATRGIVTAHDLGRMKPSA 229 + + + L + AR + K+ D + L + T + A +L MKP+A Sbjct: 174 YHNRSRLAPELEARANHARYV-SKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTA 232 Query: 230 LLVNTSRAGLIESGALEQALCAGRPGMAAVDVYEKEPLRDTQHPLLQMKNVVCTPHIGYV 289 L N +R G+++ AL AL GR A VDV+E EP + L + NVV TPHI Sbjct: 233 TLTNIARGGIVDDAALIAALREGRIAAAGVDVFENEPAFKPE--FLDLSNVVLTPHIASA 290 Query: 290 -TEDEFELQFADIFEQIVSFAAGQPIHVVNPEVL 322 T + I + + +P +++NP+V+ Sbjct: 291 STPTRLAMANCAADNLIAALSGQRPPNLLNPDVV 324 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 102 bits (253), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 84/272 (30%), Positives = 126/272 (46%), Gaps = 18/272 (6%) Query: 50 LVLIRERTQIRAPLIARLPALRLISQRSA-FPHIDIAACSTHGVIVSSNQHADTPSYAAA 108 +VL T + A + LP L+L+ A F ++D+A HG+ +++ A T A Sbjct: 47 VVLTNGSTGLTAEEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATG--AGTNEDCVA 104 Query: 109 ELTWGLILAAMRQIPQQMRALQDGFWQ--IGVGKTLRGRTLGIYGYGRIGAEVAAYGAAF 166 + GL+LA +R IP R +DG W+ I + L G+ +GI G G IG ++A AAF Sbjct: 105 DHALGLLLAILRNIPVLDRYTRDGGWRETIPLQPQLAGKRVGIVGMGNIGKKIARRAAAF 164 Query: 167 GMKVLIWASETSLMRARDD-GWDIAPDKKTFFETCDVLSLHMRLVPATRGIVTAHDLGRM 225 ++ + RDD + PD D L + TR ++ A L + Sbjct: 165 DAEIAYCNR-----KKRDDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEEL 219 Query: 226 KPSALLVNTSRAGLIESGALEQALCAGRPGMAAVDVYEKEPLRDTQHP--LLQMKNVVCT 283 P LVN R ++++ AL AL +GR A +DVYE EP Q P L+ + NVV T Sbjct: 220 GPQGYLVNIGRGSIVDTDALGAALSSGRLAGAGLDVYEGEP----QPPAALIALPNVVLT 275 Query: 284 PHIGYVTEDEFELQFADIFEQIVS-FAAGQPI 314 PHI + + FAA +P+ Sbjct: 276 PHIAGWSPEAIRASVTQFLRNCEEHFAAAEPV 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,339,477 Number of extensions: 56374 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 10 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)