BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0214.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 923 0.0 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 182 4e-47 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 162 4e-41 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 145 5e-36 gi|300313659|ref|YP_003777751.1| amidase family protein [Herbasp... 140 3e-34 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 923 bits (2385), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/463 (99%), Positives = 463/463 (100%) Query: 1 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHGP 60 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHGP Sbjct: 6 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQGGHGP 65 Query: 61 LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFG 120 LSGIPIAIKDNIDVAGIASTAGT+ALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFG Sbjct: 66 LSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFG 125 Query: 121 ITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVG 180 ITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVG Sbjct: 126 ITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVG 185 Query: 181 LRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQLRLG 240 LRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQLRLG Sbjct: 186 LRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPPSQLRLG 245 Query: 241 VLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLPTLAPLSHAAGFAIVLHEFLIDL 300 VLRNPSWQGLEEEVEANANAALMQLREAGVEL+DVDLPTLAPLSHAAGFAIVLHEFLIDL Sbjct: 246 VLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPLSHAAGFAIVLHEFLIDL 305 Query: 301 PAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCF 360 PAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCF Sbjct: 306 PAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCF 365 Query: 361 RSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGL 420 RSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGL Sbjct: 366 RSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGL 425 Query: 421 SEYGLPVGLELDGPEGSDRRLLQVAASLETVLGRGPRPPRPPR 463 SEYGLPVGLELDGPEGSDRRLLQVAASLETVLGRGPRPPRPPR Sbjct: 426 SEYGLPVGLELDGPEGSDRRLLQVAASLETVLGRGPRPPRPPR 468 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 182 bits (463), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 170/482 (35%), Positives = 242/482 (50%), Gaps = 48/482 (9%) Query: 9 GLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLD----EQGGHGPLSGI 64 G + R L Y+ + A + LN + +P+ L +A LD + G GPL GI Sbjct: 64 GRATSRSLVLAYLARIRAYDQQGPSLNAIVTLNPKALE-EADQLDRERRQSGPRGPLHGI 122 Query: 65 PIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMHELAFGITNN 124 PI +KDN D + +T GT AL Q A V+ L AGA+ GK MHELA G+T Sbjct: 123 PILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHELAAGVTTV 182 Query: 125 NGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCGVVGLRPT 184 + + G RNPYD GGSSGG GA +AA A A +G+DT GS+RIPAA + GLR T Sbjct: 183 SSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLFGLRTT 242 Query: 185 SRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGS--RDSAPLLA---LPPSQ--- 236 S+ GV+P+S T+D P+ARSV D+A++ GS +DS+ + A +P S Sbjct: 243 RGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHIPKSYRDG 302 Query: 237 --------LRLGVLRNPSWQGLEE-EVEANANAALMQLREAGVELIDVDLPTLAPLSHAA 287 R+GVLR E+ EV A N AL QL++ G + DV +P L L + Sbjct: 303 LRADSLQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGAIVTDVTIPELDGL--LS 360 Query: 288 GFAIVLHEFLIDLPAYLQASDARDV--IKRVLA-----EVGSPDVARIIQATLDQPVPEH 340 G +I+ +EF DL AYLQ+ V + +LA E+ + L P + Sbjct: 361 GSSIIPYEFKYDLGAYLQSHPGAPVGSLGEILARGMEHELLEAGLRLRNSVDLQNPKDKE 420 Query: 341 AYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFA 400 + +R +++L D + +D L++PT A +GE A+ Sbjct: 421 ELEKVMLKRSALKSLMTDVMQKNHLDTLVYPTIQRKAALIGEPQGGAM------------ 468 Query: 401 RNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLETVLGRGPRPPR 460 N ++ GLP++++PVG +E GLPV LEL PE ++ LL +A E +G P R Sbjct: 469 -NCQLSATTGLPALALPVGFTEDGLPVSLELLAPEFAEPALLGLAYGWEQKIG----PRR 523 Query: 461 PP 462 P Sbjct: 524 SP 525 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 162 bits (411), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 164/494 (33%), Positives = 234/494 (47%), Gaps = 45/494 (9%) Query: 1 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQ---GG 57 +TL E+ L R+ S E + +A DLN +L D E +QA++ D + Sbjct: 4 LTLKELSAQLQARKISATELATLFLDRIDAS-DLNAFLHVDRELTLAQAKVADSRLSDNN 62 Query: 58 HGPLSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMHEL 117 G L+G+PIA KD G +TAG+ L N V ATVVE AG + GK N E Sbjct: 63 AGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCDEF 122 Query: 118 AFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCG 177 A G N N FGP NP+D + GGSSGG A +AARLAPAA TDTGGS+R PA+LCG Sbjct: 123 AMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCG 182 Query: 178 VVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG--SRDSAPL------ 229 V G++PT S++G++ + + D GP+A++ D LL M G RDS L Sbjct: 183 VTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPKED 242 Query: 230 ----LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLPTLAPLSH 285 L LR+GV + GL +VEA AAL + + G +L+++ LP LS Sbjct: 243 FTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPK-TELSI 301 Query: 286 AAGFAIVLHEFLIDLPAY------LQASDA---RDVIKRVLAEVGSPDVARII---QATL 333 + I E +L + +A+D D+ ++ AE +V R I L Sbjct: 302 PVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVGSYVL 361 Query: 334 DQPVPEHAYRLALEQRRTMQALYRDCF--RSQGVDALIFPTTALTARPLGE--DDTVALR 389 + Y A + RR + +++ ++ D ++ P + A LG+ +D VA Sbjct: 362 SHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTNDPVANY 421 Query: 390 GERVPTFPAFARNADGASIAGLPSISIPVGLSE----YGLPVGLELDGPEGSDRRLLQVA 445 + T S+AGLP +SIP G + PVGL++ G + RLL VA Sbjct: 422 LADIFTLS--------TSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVA 473 Query: 446 ASLETVLGRGPRPP 459 + R P Sbjct: 474 HQFQQATDWHLREP 487 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 145 bits (367), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 135/411 (32%), Positives = 200/411 (48%), Gaps = 48/411 (11%) Query: 60 PLSGIPIAIKDNIDVAGIASTAGTSALRNN-VPQRHATVVEYLFGAGALHAGKANMHELA 118 PL G+P+++KD DVAG + AG+ LR V HA VV+ L AGA+ GK NM E A Sbjct: 68 PLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFA 127 Query: 119 FGITNNNGVFGPCRNPYDAEL----IAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAA 174 + N +G +N ++ + I GGSS G + +A AAVG+DTGGSVRIP+A Sbjct: 128 YSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSA 187 Query: 175 LCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLLALPP 234 L G+ G +PT+ R S GV+P+S D+ GP+A SV A++D ++AG D +LA P Sbjct: 188 LNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG-EDYVAVLAHPL 246 Query: 235 SQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELIDVDLP---TLAPLSHAAGF-- 289 LRL N G+++ V AL +L AG +++ ++P LA ++ GF Sbjct: 247 RGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINAKGGFTA 306 Query: 290 --AIVLHEFLIDLPAYLQASDARDVIKRVL--AEVGSPDVARIIQATLDQPVPEHAYRLA 345 A H LI A A + V+ R+L ++ + D+ ++ HA Sbjct: 307 AEAYAWHRALI---AENAAGYDQRVVSRILRGKDMSAADLLDVL----------HARPRW 353 Query: 346 LEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE----DDTVALRGERVPTFPAFAR 401 + Q A G DAL+ PT + A + E DD ++ P Sbjct: 354 IAQVEAQLA---------GYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLIN 404 Query: 402 NADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLETVL 452 DG ++S+P + PVGL L G D+RLL + ++E +L Sbjct: 405 FLDGC------ALSLPCH-APGSAPVGLSLAACGGQDKRLLSIGLAVEALL 448 >gi|300313659|ref|YP_003777751.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 506 Score = 140 bits (352), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 157/491 (31%), Positives = 233/491 (47%), Gaps = 60/491 (12%) Query: 4 IEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDP----EQLRSQARMLDEQGGHG 59 +E+RQ + ++ S +E ++A IA E D+N ++ E+ R +AR ++ G Sbjct: 11 VELRQLIGSKQLSPVELLEACIARIE---DINPHINAVTATCFERARGEARAAEQAVIDG 67 Query: 60 P----LSGIPIAIKDNIDVAGIASTAGTSALRNNVPQRHATVVEYLFGAGALHAGKANMH 115 L G+PI IKD + G+ +T G+ R N+P R +V L AGA+ AGK N+ Sbjct: 68 KPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAGKTNVP 127 Query: 116 ELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAAL 175 E+ G + N V+G NP++ L AGGSSGG A LA L P G+DTGGS+RIPAA Sbjct: 128 EMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLRIPAAK 187 Query: 176 CGVVGLRPT-----SRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPL- 229 CGVVG RP+ S R G PIS GPM R VAD L + G S PL Sbjct: 188 CGVVGFRPSPGLVPSER-KLLGWTPISVV----GPMGRDVADTVLQLRATLGLHASDPLG 242 Query: 230 --------LALPP---SQLRLGVLRNPSWQGLEE---EVEANANAALMQLREAGVELIDV 275 +LP SQLR+G + ++ +V + AA+ + E +DV Sbjct: 243 YAVSDSEFASLPQVDLSQLRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYVKL-CEPVDV 301 Query: 276 DLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQ 335 D+ +H A F ++ E A++ DA +R A +G P+ + Sbjct: 302 DMGE----AHRA-FDVIRAE------AFVAGFDA--AYRRDPASLG-PNTRANYEMGAAM 347 Query: 336 PVPEHAYRLALEQR--RTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRGERV 393 + + A+ + + R R Q LY + D ++ PTT ++ P E + G + Sbjct: 348 SLLDCAWAQSEQTRIFRRFQQLY------ERYDLILSPTTPVSPFPWSELYLKEVNGVAL 401 Query: 394 PTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLETVLG 453 + + ++A P+IS+P G G+P GL++ GP D R+L AA+LE Sbjct: 402 ENYYRWLALTYVVTLATNPAISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQAFA 461 Query: 454 RGPRPPRPPRP 464 G R PRP Sbjct: 462 -GNETLRRPRP 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,397,897 Number of extensions: 58641 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 11 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)