BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0186.p (330 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311736|ref|YP_003775828.1| myo-inositol 2-dehydrogenase [H... 667 0.0 gi|300311737|ref|YP_003775829.1| oxidoreductase domain-containin... 165 5e-42 gi|300313480|ref|YP_003777572.1| dehydrogenase [Herbaspirillum s... 60 3e-10 >gi|300311736|ref|YP_003775828.1| myo-inositol 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 667 bits (1721), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/330 (99%), Positives = 329/330 (99%) Query: 1 MIDVAIFGAGRIGKIHAANIAAQAGVRLKYVCDVHAESAAELAQRHGAQVGRVEQVLDDS 60 MIDVAIFGAGRIGKIHAANIAAQAGVRLKYVCDVHAESAAELAQRHGAQVGRVEQVLDDS Sbjct: 1 MIDVAIFGAGRIGKIHAANIAAQAGVRLKYVCDVHAESAAELAQRHGAQVGRVEQVLDDS 60 Query: 61 SVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQACAEAVRAAGVTCMIGFQ 120 SVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQACAEAVRAAGVTCMIGFQ Sbjct: 61 SVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQACAEAVRAAGVTCMIGFQ 120 Query: 121 RRFDPTFESLSRRLEAGEIGDPEMLLVTSRDPGAPPLSYIASSGGIFKDMLIHDFDIFRW 180 RRFDPTFESLSRRLEAGEIGDPEMLLVTSRDPGAPPLSYIASSGGIFKDMLIHDFDIFRW Sbjct: 121 RRFDPTFESLSRRLEAGEIGDPEMLLVTSRDPGAPPLSYIASSGGIFKDMLIHDFDIFRW 180 Query: 181 ILADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAAYGYDQRFEV 240 ILADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAAYGYDQRFEV Sbjct: 181 ILADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAAYGYDQRFEV 240 Query: 241 LGSKGMLQAGNHRPTEVVAATASQVSSDLPEHFFLERYRAAYAREMAHFFQAMQSGAPVR 300 LGSKGMLQAGNHRPTEVVAATASQV SDLPEHFFLERYRAAYAREMAHFFQAMQSGAPVR Sbjct: 241 LGSKGMLQAGNHRPTEVVAATASQVGSDLPEHFFLERYRAAYAREMAHFFQAMQSGAPVR 300 Query: 301 TSIEDGVKALQLAEAAATSWRERRIVSLSA 330 TSIEDGVKALQLAEAAATSWRERRIVSLSA Sbjct: 301 TSIEDGVKALQLAEAAATSWRERRIVSLSA 330 >gi|300311737|ref|YP_003775829.1| oxidoreductase domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 341 Score = 165 bits (417), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 112/337 (33%), Positives = 174/337 (51%), Gaps = 12/337 (3%) Query: 2 IDVAIFGAGRIGKIHAANIAAQA-GVRLKYVCDVHAESAAELAQRHGAQVGRVEQ---VL 57 + V + G GR+G+ HA NIA G L VC A+ + AQ+H R E +L Sbjct: 7 LQVGLVGLGRMGRRHAENIAFNTPGATLCAVCSPLAQER-DWAQQHLPSAERYEDYQALL 65 Query: 58 DDSSVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQ-ACAEAVRAAGVTCM 116 + AV + + T H + I+ A AGK +FCEKP+ LDL+ Q AEA R + M Sbjct: 66 RHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEECQRVAAEAARHPQLKVM 125 Query: 117 IGFQRRFDPTFESLSRRLEAGEIGDPEMLLVTS---RDPGAPPLSYIASSGGIFKDMLIH 173 IGF RRFD +++ R++ G IG P M+ + DP + + ASSGG+F D +H Sbjct: 126 IGFVRRFDASYQDAHRKISEGLIGAPFMVYSQTADALDPDGFFVRFAASSGGLFLDCSVH 185 Query: 174 DFDIFRWIL-ADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAAY 232 D D+ RW+L AD+ V V A+G + D+D+ GRL SR A+ Sbjct: 186 DIDLARWLLGADQPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFYASRTMAH 245 Query: 233 GYDQRFEVLGSKGMLQAG-NHRPTEVVAATASQVSSDLPEHFFLERYRAAYAREMAHFFQ 291 G++ E++G++G L G N + A A + ++ F+ER+ A+ E+ HF Sbjct: 246 GHETMTEIVGTRGRLSVGANPSLNRLTIADAHGLRNECTPS-FIERFAEAFVAEVRHFVD 304 Query: 292 AMQSGAPVRTSIEDGVKALQLAEAAATSWRERRIVSL 328 A++ P+ +++D ++A ++A A S E++ V L Sbjct: 305 AVRYDTPLALTLQDAIEATRIAAAMRQSLLEQQPVQL 341 >gi|300313480|ref|YP_003777572.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 352 Score = 59.7 bits (143), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 80/352 (22%), Positives = 138/352 (39%), Gaps = 40/352 (11%) Query: 2 IDVAIFGAGRIGKIHAANIAAQAG-VRLKYVCDVHAESAAELAQRHGAQ-VGRVEQVLDD 59 I + I G GRI K H +I A + L +CD+ + AE A+++ +E++L+ Sbjct: 12 IRIGIVGCGRISKNHFGSIEKHADEMELIAICDIDPVTLAEHAEKYNVPAYSTMEEMLEK 71 Query: 60 SSVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQACAEAVRAAGVTCMIGF 119 + V + + + +H + + AA + EKP+ + +A AGV + Sbjct: 72 EQLDLVALCTPSGSHPEQTVLAAKHRIHVMTEKPMATRWKDGVRMVKACDEAGVHLFVVK 131 Query: 120 QRRFDPTFESLSRRLEAGEIGDPEMLLV--------TSRDPGAPPLSYIASSGGIFKDML 171 Q R + T + L R + G M+ + + D G GG F + Sbjct: 132 QNRRNTTLQLLKRAVTEKRFGKIHMVHLNVFWTRPQSYYDQGNGWRGTWEFDGGAFMNQA 191 Query: 172 IHDFDIFRWILADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAA 231 H D+ W++ V A S D + D+ + + R+G L ++ + Sbjct: 192 SHYVDLLDWLIG-PVEKVQAMMSTTRDIEVE-----DTGVLNVKWRNGALGSMSVTMLT- 244 Query: 232 YGYDQRFE----VLGSKGMLQAGNHRPTEVVAATASQVSS-DLPEHFFLERYRAAYAREM 286 Y + E +LG KG ++ G VA Q+ + P + + A Y Sbjct: 245 --YPKNLEGSITILGEKGTVRVGG------VAVNDIQLWQFEEPRDYDHQIQEANYETTS 296 Query: 287 AHFF----------QAMQSGAPVRTSIEDGVKALQLAEAAATSWRERRIVSL 328 + F M+ A T +G+K+L+L AA S R+ VSL Sbjct: 297 VYGFGHPLYYKNVIDVMRGLAAPETDGREGLKSLELLIAAYLSARDGTTVSL 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 903,099 Number of extensions: 31960 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 3 Length of query: 330 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 244 Effective length of database: 1,212,660 Effective search space: 295889040 Effective search space used: 295889040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)