BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0185.p (341 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311737|ref|YP_003775829.1| oxidoreductase domain-containin... 688 0.0 gi|300311736|ref|YP_003775828.1| myo-inositol 2-dehydrogenase [H... 159 3e-40 gi|300313480|ref|YP_003777572.1| dehydrogenase [Herbaspirillum s... 79 5e-16 >gi|300311737|ref|YP_003775829.1| oxidoreductase domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 341 Score = 688 bits (1775), Expect = 0.0, Method: Compositional matrix adjust. Identities = 337/341 (98%), Positives = 338/341 (99%) Query: 1 MSAPASLQVGLVGLGRMGRRHAENIAFNTPGATLCAVCSPLAQERDWAQQHLPSAERYED 60 MSAPASLQVGLVGLGRMGRRHAENIAFNTPGATLCAVCSPLAQERDWAQQHLPSAERYED Sbjct: 1 MSAPASLQVGLVGLGRMGRRHAENIAFNTPGATLCAVCSPLAQERDWAQQHLPSAERYED 60 Query: 61 YPALLRHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEECHRVAAEAARHP 120 Y ALLRHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEEC RVAAEAARHP Sbjct: 61 YQALLRHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEECQRVAAEAARHP 120 Query: 121 QLKVMIGFVRRFDASYQDAHRKISQGLIGAPFMVYSQTADALDPDGFFVRFAASSGGLFL 180 QLKVMIGFVRRFDASYQDAHRKIS+GLIGAPFMVYSQTADALDPDGFFVRFAASSGGLFL Sbjct: 121 QLKVMIGFVRRFDASYQDAHRKISEGLIGAPFMVYSQTADALDPDGFFVRFAASSGGLFL 180 Query: 181 DCSVHDIDLARWLLGAVQPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFYAS 240 DCSVHDIDLARWLLGA QPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFYAS Sbjct: 181 DCSVHDIDLARWLLGADQPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFYAS 240 Query: 241 RTMAHGHETMTEIVGTRGRLSVGANPSLNRLTIADAHGLRNECTPSFIERFAEAFVAEVR 300 RTMAHGHETMTEIVGTRGRLSVGANPSLNRLTIADAHGLRNECTPSFIERFAEAFVAEVR Sbjct: 241 RTMAHGHETMTEIVGTRGRLSVGANPSLNRLTIADAHGLRNECTPSFIERFAEAFVAEVR 300 Query: 301 HFVDAVRYDTPLALTLQDAIEATRIAAAMRQSLLEQQPVQL 341 HFVDAVRYDTPLALTLQDAIEATRIAAAMRQSLLEQQPVQL Sbjct: 301 HFVDAVRYDTPLALTLQDAIEATRIAAAMRQSLLEQQPVQL 341 >gi|300311736|ref|YP_003775828.1| myo-inositol 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 110/337 (32%), Positives = 172/337 (51%), Gaps = 12/337 (3%) Query: 7 LQVGLVGLGRMGRRHAENIAFNTPGATLCAVCSPLAQER-DWAQQHLPSAERYEDYPALL 65 + V + G GR+G+ HA NIA G L VC A+ + AQ+H R E +L Sbjct: 2 IDVAIFGAGRIGKIHAANIAAQA-GVRLKYVCDVHAESAAELAQRHGAQVGRVEQ---VL 57 Query: 66 RHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEECHRVAAEAARHPQLKVM 125 + AV + + T H + I+ A AGK +FCEKP+ LDL+ AEA R + M Sbjct: 58 DDSSVAAVVIASSTDTHADLILRAAAAGKAIFCEKPVDLDLQRAQ-ACAEAVRAAGVTCM 116 Query: 126 IGFVRRFDASYQDAHRKISQGLIGAPFMVYSQTADALDPDGFFVRFAASSGGLFLDCSVH 185 IGF RRFD +++ R++ G IG P M+ + DP + + ASSGG+F D +H Sbjct: 117 IGFQRRFDPTFESLSRRLEAGEIGDPEMLLVTS---RDPGAPPLSYIASSGGIFKDMLIH 173 Query: 186 DIDLARWLLGAVQPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFYASRTMAH 245 D D+ RW+L A + V V A+G + D+D+ GRL SR A+ Sbjct: 174 DFDIFRWIL-ADEAVSVFATGSCLTDPAIATAGDVDSTAVTLTTRSGRLCQINTSRRAAY 232 Query: 246 GHETMTEIVGTRGRLSVGANPSLNRLTIADAHGLRNECTPS-FIERFAEAFVAEVRHFVD 304 G++ E++G++G L G N + A A + ++ F+ER+ A+ E+ HF Sbjct: 233 GYDQRFEVLGSKGMLQAG-NHRPTEVVAATASQVGSDLPEHFFLERYRAAYAREMAHFFQ 291 Query: 305 AVRYDTPLALTLQDAIEATRIAAAMRQSLLEQQPVQL 341 A++ P+ +++D ++A ++A A S E++ V L Sbjct: 292 AMQSGAPVRTSIEDGVKALQLAEAAATSWRERRIVSL 328 >gi|300313480|ref|YP_003777572.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 352 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 65/267 (24%), Positives = 116/267 (43%), Gaps = 19/267 (7%) Query: 6 SLQVGLVGLGRMGRRHAENIAFNTPGATLCAVCS--PLAQERDWAQQHLPSAERYEDYPA 63 +++G+VG GR+ + H +I + L A+C P+ + ++P+ Y Sbjct: 11 KIRIGIVGCGRISKNHFGSIEKHADEMELIAICDIDPVTLAEHAEKYNVPA---YSTMEE 67 Query: 64 LLRHPGLDAVFLVTPTALHGEQIIAALRAGKHVFCEKPLSLDLEECHRVAAEAARHPQLK 123 +L LD V L TP+ H EQ + A + HV EKP++ ++ R+ +A + Sbjct: 68 MLEKEQLDLVALCTPSGSHPEQTVLAAKHRIHVMTEKPMATRWKDGVRMV-KACDEAGVH 126 Query: 124 VMIGFVRRFDASYQDAHRKISQGLIGAPFMV-----YSQTADALDPDGFFVRFAASSGGL 178 + + R + + Q R +++ G MV +++ D + GG Sbjct: 127 LFVVKQNRRNTTLQLLKRAVTEKRFGKIHMVHLNVFWTRPQSYYDQGNGWRGTWEFDGGA 186 Query: 179 FLDCSVHDIDLARWLLGAVQPVRVMASGVRAVHHELEAFQDIDNGVAICEFAQGRLATFY 238 F++ + H +DL WL+G V+ V+ M S R + E D GV ++ G L + Sbjct: 187 FMNQASHYVDLLDWLIGPVEKVQAMMSTTRDIEVE-------DTGVLNVKWRNGALGSMS 239 Query: 239 ASR-TMAHGHETMTEIVGTRGRLSVGA 264 + T E I+G +G + VG Sbjct: 240 VTMLTYPKNLEGSITILGEKGTVRVGG 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,059,284 Number of extensions: 43784 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 3 Length of query: 341 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 255 Effective length of database: 1,212,660 Effective search space: 309228300 Effective search space used: 309228300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)