BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0181.p (266 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311741|ref|YP_003775833.1| sugar ABC transporter ATPase [H... 531 e-152 gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [H... 171 6e-44 gi|300310396|ref|YP_003774488.1| sugar ABC transporter ATPase [H... 165 3e-42 gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [H... 164 6e-42 gi|300310367|ref|YP_003774459.1| sugar ABC transporter ATPase [H... 164 8e-42 >gi|300311741|ref|YP_003775833.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 531 bits (1369), Expect = e-152, Method: Compositional matrix adjust. Identities = 261/263 (99%), Positives = 263/263 (100%) Query: 4 MLRSDEVILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQA 63 MLRSDE+ILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQA Sbjct: 1 MLRSDEIILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQA 60 Query: 64 SSGQYLVDGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGITVM 123 SSGQYLVDGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFG+TVM Sbjct: 61 SSGQYLVDGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGVTVM 120 Query: 124 DMELAARTAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAAL 183 DMELAARTAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAAL Sbjct: 121 DMELAARTAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAAL 180 Query: 184 GVKQSFNVLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEE 243 GVKQSFNVLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEE Sbjct: 181 GVKQSFNVLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEE 240 Query: 244 VLDMMAGGAEMQTLMGQLEGIQV 266 VLDMMAGGAEMQTLMGQLEGIQV Sbjct: 241 VLDMMAGGAEMQTLMGQLEGIQV 263 >gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 171 bits (433), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 92/242 (38%), Positives = 146/242 (60%), Gaps = 3/242 (1%) Query: 11 ILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLV 70 ++ L V K F V+AL N L GEVH L+G+NGAGKSTL+K+L+GV+Q SG L+ Sbjct: 22 VIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILL 81 Query: 71 DGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGITVMDMELAAR 130 DGKPV P++A +G+ ++Q+L L+ LS A+N F+GREP RK G+ + + EL R Sbjct: 82 DGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREP-RKAMGLFIDEDEL-NR 139 Query: 131 TAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFN 190 A A M + + D P+G ++ +Q + IA+A+ F +RVLI+DEPTAAL + Sbjct: 140 QAAAIFARMRLDM-DPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAE 198 Query: 191 VLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDMMAG 250 + ++I + +G+ +++I+H + I D +++ GK + + S + ++ MM G Sbjct: 199 LFRIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVG 258 Query: 251 GA 252 A Sbjct: 259 RA 260 Score = 67.8 bits (164), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 48/225 (21%), Positives = 102/225 (45%), Gaps = 5/225 (2%) Query: 26 ALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLVDGKPVRFESPKEALD 85 A+++VS LR+GE+ G GAG++ + + + G +G+ ++ G +SP +A+ Sbjct: 288 AIRDVSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVA 347 Query: 86 VGVATVYQD---LALVPLLSVARNFFLGREPVRKLFGITVMDMELAARTAKEKLAEMGIM 142 G+ + +D L + V N L + + + MD A+ + ++ I Sbjct: 348 HGIGYLSEDRKHFGLAVGMDVQANIALS--SMGRFTRVGFMDQRAIREAAQMYVRQLAIK 405 Query: 143 VRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFNVLKLIHRARERG 202 +SGG +Q + IA+ + +L DEPT + V + KL+ E+G Sbjct: 406 TPSVEQQARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQG 465 Query: 203 ISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDM 247 +++ I+ + + ++ G+ G A+ ++E+++ + Sbjct: 466 KAIVMISSELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQL 510 >gi|300310396|ref|YP_003774488.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 165 bits (418), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 88/245 (35%), Positives = 150/245 (61%), Gaps = 8/245 (3%) Query: 8 DEVILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQ 67 + +L+L + K FGPV L+ V L +R GEVH LLG+NGAGKSTL+K+L G+ + +G+ Sbjct: 2 EAAMLQLTGIKKNFGPVTVLRGVDLEVRAGEVHALLGENGAGKSTLMKILCGIVRPDAGE 61 Query: 68 YLVDGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGITVMDMEL 127 +DG+P RF+S + A+ GV V+Q+ +L+P L N FL RE +R +G ++ Sbjct: 62 IRIDGQPCRFDSYRAAIAGGVGVVFQEFSLIPYLDAVDNMFLARE-LRSRWGW----LQR 116 Query: 128 AA--RTAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGV 185 AA R A+E + ++G+ + P+ +S ++Q + IA+A+ AR+L+LDEPTA L Sbjct: 117 AAMRRRAQEIIGQLGVAI-PLDVPVCKLSVAQQQFVEIAKALALDARILVLDEPTATLTP 175 Query: 186 KQSFNVLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVL 245 + ++ ++ R +G+++IFI+H++ + I D T+L G + + A + + ++ Sbjct: 176 AEVEHLFAVMRSLRAQGVAIIFISHHLEEIFEICDRITVLRDGAYVATCATAEVDQARLV 235 Query: 246 DMMAG 250 +MM G Sbjct: 236 EMMVG 240 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 60/241 (24%), Positives = 112/241 (46%), Gaps = 10/241 (4%) Query: 9 EVILELDQVS-KFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQ 67 EV+LE+ + + PV Q LRRGE+ G G+G++ + + G H A S + Sbjct: 260 EVVLEVHALQLRRQAPVSQFQ-----LRRGEILGFAGLVGSGRTETVLAMLGAHAALSCK 314 Query: 68 YLVDGKPVRFESPKEALDVGVATV---YQDLALVPLLSVARNFFLGREPVRKLFGITVMD 124 + G P+RF P +AL G+ + ++ L+ S+ N L +L G+ +D Sbjct: 315 LSMHGVPLRFADPAQALQAGIGLLPESRKEQGLITSFSILHNVSLNNYGKYRLGGL-FLD 373 Query: 125 MELAARTAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALG 184 + + + + + A + T+SGG +Q + IAR I+ +VLI DEPT + Sbjct: 374 RRREQQATEAAMQRVRVKAPGAQVRVDTLSGGNQQKVVIARWINHAMKVLIFDEPTRGID 433 Query: 185 VKQSFNVLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEV 244 V + +L+ +G S++ I+ + + D + G + + + ++ EE+ Sbjct: 434 VGAKSEIYQLMREFTAQGYSILMISSELPEVVGMADRVCVFRGGGIVATLEGEAVNAEEI 493 Query: 245 L 245 + Sbjct: 494 M 494 >gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 521 Score = 164 bits (415), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 87/240 (36%), Positives = 143/240 (59%), Gaps = 4/240 (1%) Query: 11 ILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLV 70 +L++ + K FG +AL ++ L +R GE+H L+G+NGAGKSTL+K+L+GVH G+ L+ Sbjct: 6 LLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILL 65 Query: 71 DGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGITVMDMELAAR 130 DG+PV P + G+ +YQ+LA+ P +SVA N F+G E +R G+ +D Sbjct: 66 DGRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSE-LRTRLGL--IDHAAMRS 122 Query: 131 TAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFN 190 L ++G A G +S E+Q + IARA+ +R++I+DEPTAAL +++ Sbjct: 123 RTDAVLRQLGAGF-GASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQ 181 Query: 191 VLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDMMAG 250 + ++ R R+ G+++I+I+H + Y + D T+L G +G +D I E ++ MM G Sbjct: 182 LFNVVRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVG 241 Score = 97.1 bits (240), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 68/233 (29%), Positives = 115/233 (49%), Gaps = 23/233 (9%) Query: 27 LQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLVDGKPVRFESPKEALDV 86 ++ S +R GEV G GAG++ L +LL G S G L++G+PV + P+ A+ Sbjct: 275 IRPASFDVRAGEVLGFAGLVGAGRTELARLLFGADPRSGGDILLEGRPVHIDQPRAAMRA 334 Query: 87 GVATVYQD-----------LALVPLLSVA-RNFFLGREPVRKLFGITVMDMELAARTAKE 134 G+A V +D +A ++VA R+ LG R L G+ AR A + Sbjct: 335 GIAYVPEDRKGQGLFLQMAVAANATMNVASRHTRLGLVRSRSLGGV--------ARAAIQ 386 Query: 135 KLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFNVLKL 194 +L + V P+G +SGG +Q + +AR + +VLILDEPT + + + +L Sbjct: 387 RL---NVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVLILDEPTRGVDIYAKSEIYQL 443 Query: 195 IHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDM 247 +HR +G++V+ I+ + I D ++ G G A I++E ++ + Sbjct: 444 VHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGELAGAAITQENIMRL 496 >gi|300310367|ref|YP_003774459.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 516 Score = 164 bits (414), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 95/240 (39%), Positives = 147/240 (61%), Gaps = 5/240 (2%) Query: 11 ILELDQVSKFFGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLV 70 +LEL + K F V AL +V LRL GEVH L+G NGAGKSTLIK+L GVH+ G+ + Sbjct: 12 MLELSGIHKAFAGVKALTDVGLRLFPGEVHTLMGQNGAGKSTLIKVLTGVHEPDGGKIEL 71 Query: 71 DGKPVRFESPKEALDVGVATVYQDLALVPLLSVARNFFLGREPVRKLFGITVMDMELAAR 130 DG+ + S EA +G++TVYQ++ L P LSVA N F+GR P R FG +D + Sbjct: 72 DGRAIAPASTLEAQALGISTVYQEVNLCPNLSVAENIFVGRYPTR--FG--RIDWKSVHT 127 Query: 131 TAKEKLAEMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFN 190 +++ L ++ I + D P+ + +Q +AI+RA+ A+VLILDEPT++L + Sbjct: 128 QSRQLLQQLQIDI-DVAAPLSSYPLAIQQMVAISRALSVSAKVLILDEPTSSLDEAEVQQ 186 Query: 191 VLKLIHRARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDMMAG 250 + K++ R RE+G++++F+TH + Y I D T+L G G + +S+ ++++ M G Sbjct: 187 LFKVLRRLREQGMAILFVTHFLDQTYEISDRITVLRNGVREGEYLVSELSRLDLVNKMVG 246 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 68/233 (29%), Positives = 117/233 (50%), Gaps = 5/233 (2%) Query: 21 FGPVIALQNVSLRLRRGEVHCLLGDNGAGKSTLIKLLAGVHQASSGQYLVDGKPVRFESP 80 FG L L+LRRGEV L G G+G++ + +LL G +A GQ ++GK VR P Sbjct: 283 FGRRGVLAPQDLQLRRGEVFGLCGLLGSGRTEMARLLFGADRADCGQLRIEGKEVRLNVP 342 Query: 81 KEALDVGVATVYQDL---ALVPLLSVARNFFLGREPVRKLFGITVMDMELAARTAKEKLA 137 ++A+ G+ +D + LSV N L + + LF V+ + + A + + Sbjct: 343 RDAIAAGIGFCSEDRKKEGAILELSVRENIILALQARQGLF--RVLPRKRQNQIAADYVK 400 Query: 138 EMGIMVRDAHHPIGTMSGGEKQCLAIARAIHFGARVLILDEPTAALGVKQSFNVLKLIHR 197 +GI D PIG +SGG +Q +AR + +LILDEPT + V+ ++ + Sbjct: 401 WLGIKTADLETPIGLLSGGNQQKALLARWLATDPGMLILDEPTRGIDVRAKQEIMDFVIA 460 Query: 198 ARERGISVIFITHNVHHAYPIGDTFTLLNRGKSLGSFAKDRISKEEVLDMMAG 250 +G+S++FI+ + D +L ++ G + + + ++ VL ++AG Sbjct: 461 MCRKGMSILFISSEIPEVLRCSDRMLVLRDRRACGEYRRGELDEQSVLQVIAG 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 794,667 Number of extensions: 33244 Number of successful extensions: 486 Number of sequences better than 1.0e-08: 128 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 145 Length of query: 266 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 182 Effective length of database: 1,222,130 Effective search space: 222427660 Effective search space used: 222427660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)