BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0180.p (657 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspiril... 1341 0.0 gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbasp... 94 4e-20 >gi|300311742|ref|YP_003775834.1| transferase kinase [Herbaspirillum seropedicae SmR1] Length = 657 Score = 1341 bits (3471), Expect = 0.0, Method: Compositional matrix adjust. Identities = 654/657 (99%), Positives = 655/657 (99%) Query: 1 MQATHTPSTSTPRRFAADRRLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIA 60 MQATHTPSTSTPRRFAADRRLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIA Sbjct: 1 MQATHTPSTSTPRRFAADRRLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIA 60 Query: 61 FGCARLGLKSAMLARIGDEANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSF 120 FGCARLGLKSAMLARIGDEANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSF Sbjct: 61 FGCARLGLKSAMLARIGDEANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSF 120 Query: 121 PLIFYRDNCADMALAPEDIDEAFIASSKALLITGTHFSTPEVHRSSSLALQYARRHNVRT 180 PLIFYRDNCADMALAPEDIDEAFIASSKALLITGTHFSTP+VHRSSSLALQYARRHNVRT Sbjct: 121 PLIFYRDNCADMALAPEDIDEAFIASSKALLITGTHFSTPQVHRSSSLALQYARRHNVRT 180 Query: 181 VLDIDYRPVLWGLTSKGDGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIM 240 VLDIDYRPVLWGLTSKGDGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIM Sbjct: 181 VLDIDYRPVLWGLTSKGDGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDIM 240 Query: 241 AALRAVRALCAATLVVKRGPLGCVVLHGAIPERLDDGFHYRGVQVEVLNVLGAGDAFISG 300 AALRAVRALCAATLVVKRGPLGCVVLHGAIPERLDDGFHYRGVQVEVLNVLGAGDAFISG Sbjct: 241 AALRAVRALCAATLVVKRGPLGCVVLHGAIPERLDDGFHYRGVQVEVLNVLGAGDAFISG 300 Query: 301 FLKGWLNEEDDEHCCRYANACGALVVSRHACAPAMPGPIELDYFLQHAHRLQRPDRDAHL 360 FLKGWLNEEDDEHCCRYANACGALVVSRHACAPAMPGPIELDYFLQHAHRLQRPDRDAHL Sbjct: 301 FLKGWLNEEDDEHCCRYANACGALVVSRHACAPAMPGPIELDYFLQHAHRLQRPDRDAHL 360 Query: 361 QRLHRVVSRRRHWDEVNVFAFDHRNQFFELAVEAGVSQDKLPALKRLLVEAVAQTEAKLG 420 QRLHRVVSRRRHWDEVNVFAFDHRNQFFELAVEAGVSQDKLPALKRLLVEAVAQTEAKLG Sbjct: 361 QRLHRVVSRRRHWDEVNVFAFDHRNQFFELAVEAGVSQDKLPALKRLLVEAVAQTEAKLG 420 Query: 421 LQGKTGVLIDDRYGQDALNAATGRGWWVGRAVELPLSNPLEFDFGRSIGTHLQTWPQEQV 480 LQGKTGVLIDDRYGQDALNAATGRGWWVGRAVELPLSNPLEFDFGRSIGTHLQTWPQEQV Sbjct: 421 LQGKTGVLIDDRYGQDALNAATGRGWWVGRAVELPLSNPLEFDFGRSIGTHLQTWPQEQV 480 Query: 481 VKCLVQFHPDEQVEKRLEQEAQIRALYQAVQRSGHELLLEIIPSKTLPQEEDTVLRALKR 540 VKCLVQFHPDEQVEKRLEQEAQIRALYQAVQRSGHELLLEIIPSKTLPQEEDTVLRALKR Sbjct: 481 VKCLVQFHPDEQVEKRLEQEAQIRALYQAVQRSGHELLLEIIPSKTLPQEEDTVLRALKR 540 Query: 541 LYNLDIYPEWWKLESMSARQWEVIDALIAERDPYCRGVVLLGLAAPVSELAAGFAASRHS 600 LYNLDIYPEWWKLESMSARQWE IDALIAERDPYCRGVVLLGLAAPVSELAAGFAASRHS Sbjct: 541 LYNLDIYPEWWKLESMSARQWEAIDALIAERDPYCRGVVLLGLAAPVSELAAGFAASRHS 600 Query: 601 KTCRGFMVGRTIFHESSRAWLQGRIDDATLIAQVRTTFEELIGLWRASRVAPAREAA 657 KTCRGFMVGRTIFHESSRAWLQGRIDDATLIAQVRTTFEELIGLWRASRV PAREAA Sbjct: 601 KTCRGFMVGRTIFHESSRAWLQGRIDDATLIAQVRTTFEELIGLWRASRVTPAREAA 657 >gi|300310831|ref|YP_003774923.1| 2-ketogluconate kinase [Herbaspirillum seropedicae SmR1] Length = 313 Score = 93.6 bits (231), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 87/328 (26%), Positives = 142/328 (43%), Gaps = 26/328 (7%) Query: 20 RLDLICIGRLAVDLYAQQVGAPLSDVSSFAKYLGGSSANIAFGCARLGLKSAMLARIGDE 79 +LD++ G L A +VG P +V + + L G+ N+A G ARLGLK +R+G++ Sbjct: 4 QLDVVTWGEALALLVADEVG-PFEEVEKYTRRLAGAETNVAIGLARLGLKVGWASRVGND 62 Query: 80 ANGQFLKAELAAEGCDVSHVTVDPQRLTALVMLGLKDKDSFPLI-FYRDNCADMALAPED 138 A G+F++ +A EG +VS V D + TA+ + + P I +YR A L+ +D Sbjct: 63 AFGRFIRQRVAQEGVEVSRVITDMEFRTAIQLKAKAVGGADPAIEYYRKGSAASHLSVDD 122 Query: 139 IDEAFIASSKALLITGTHFSTPEVHRS-SSLALQYARRHNVRTVLDIDYRPVLWGLTSKG 197 D + +++ L TG + + + A+ + R D + RP+LW Sbjct: 123 FDAGYFGAARHLHATGIAPALSATTMAFAHQAMDFMRGQGKTISFDPNLRPMLW------ 176 Query: 198 DGETRFIASDGVTQHLQGILPQFDLVVGTEEEFMIAGGSTDI--MAALRAVRALCAATLV 255 + + + Q L + + D V+ E I G D +A R + +V Sbjct: 177 ------PSQEVMAQQLNALAFKADWVLPGLSEGKILTGHDDAREIAGFYLERGV--KLVV 228 Query: 256 VKRGPLGCVVLHGAIPERLDDGFHYRGVQV-EVLNVLGAGDAFISGFLKGWLNEEDDEHC 314 +K G G +G R+ GV V EV++ +GAGD F G + G L Sbjct: 229 IKLGAEGAYWRNGQGEGRV------AGVPVKEVVDTVGAGDGFAVGVISGMLEGLPVPQA 282 Query: 315 CRYANACGALVVSRHACAPAMPGPIELD 342 N GA + +P EL+ Sbjct: 283 VMRGNRIGAFAIQVVGDMEGLPTRAELE 310 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,083,897 Number of extensions: 87820 Number of successful extensions: 265 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 261 Number of HSP's successfully gapped: 3 Length of query: 657 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 566 Effective length of database: 1,188,985 Effective search space: 672965510 Effective search space used: 672965510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)