BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0174.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 860 0.0 gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 248 5e-67 gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 66 4e-12 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 860 bits (2222), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/435 (99%), Positives = 432/435 (99%) Query: 1 MTSPLRSLRDWLVRHAPSRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQ 60 MTSPLRSLRDWLVRHAPSRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQ Sbjct: 1 MTSPLRSLRDWLVRHAPSRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQ 60 Query: 61 LQLAQPAPGQSAQAMMDPYLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPAIDPAT 120 LQLAQPAPGQSAQAMMDPYLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPA DPAT Sbjct: 61 LQLAQPAPGQSAQAMMDPYLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPATDPAT 120 Query: 121 GKPYRLGYDEILVNPYTGVVQGVRDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGT 180 GKPYRLGYDEILVNPYTGVVQGVRDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGT Sbjct: 121 GKPYRLGYDEILVNPYTGVVQGVRDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGT 180 Query: 181 VGLLWTLDCFVGMILTWPRARPFLVKWKPAWLIKWRAGFYRINLDLHRAFGLWCWVMLLL 240 VGLLWTLDCFVGMILTWPRARPFLVKWKPAWLIKWRAGFYR+NLDLHRAFGLWCWVMLLL Sbjct: 181 VGLLWTLDCFVGMILTWPRARPFLVKWKPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLL 240 Query: 241 FAWSSVMLNLRDQIYLPVMSRILSFDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGF 300 FAWSSVMLNLRDQIYLPVMSRILSFDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGF Sbjct: 241 FAWSSVMLNLRDQIYLPVMSRILSFDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGF 300 Query: 301 ARQRGLVIDFEEKLAFDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGD 360 ARQRGLVIDFEEKL FDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGD Sbjct: 301 ARQRGLVIDFEEKLDFDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGD 360 Query: 361 RDGNTFSNWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAAHRLQLRSPG 420 RDGNTFSNWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAA RLQLRSPG Sbjct: 361 RDGNTFSNWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAARRLQLRSPG 420 Query: 421 DGRKPSASHSDVASR 435 DGRKPSASHSDVASR Sbjct: 421 DGRKPSASHSDVASR 435 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 248 bits (634), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 158/427 (37%), Positives = 234/427 (54%), Gaps = 25/427 (5%) Query: 19 RHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDP 78 R LVLLHRW GL A FL + LTG+++ +D ELDAWL PQL A+ A A A M Sbjct: 2 RATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMPG 61 Query: 79 YLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPAIDPATGKPYRLGYDEILVNPYTG 138 L +V QA P++ ++ ++ A PG T+ +P +DPATGK Y L Y++I V+P+T Sbjct: 62 LELARRVEQAE-PRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTA 120 Query: 139 VVQGVRDRDKISLKPADLMPFLFKLHHSLALPR----APGALLLGTVGLLWTLDCFVGMI 194 +QG R ISL +L+PFL+KLH+++ LP G LL+G +G++W LDC + + Sbjct: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 Query: 195 LTWPRARPFLVKWKPAWLIKWRAGFYRINLDLHRAFGLWCWVMLLLFAWSSVMLNLRDQI 254 L++P R W+ + +WR G +++N DLHR+ G+W W +LL+ A +SV +NL Q+ Sbjct: 181 LSFPNWR----SWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQV 236 Query: 255 YLPVMSRILS-----FDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGFARQRGLVID 309 P++ + + FDT P P+ ++ P + +A AL + AR+ GL + Sbjct: 237 MRPLLQTLSTLTPSPFDTR---TPTAPEKVAEPRITREQAVALGQAQ----ARKMGLT-E 288 Query: 310 FEEKLAFDRRRGVY--IYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFS 367 + + GVY Y N +GN L DA +G G +P G+ F Sbjct: 289 PVGGVFYSGMFGVYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFL 348 Query: 368 NWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAAHRLQLRSPGDGRKPSA 427 LH G + GLP RIL+ +G V++L+VTGV++W +KR A RLQ R +K A Sbjct: 349 QAQFPLHSGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKA-RLQQRERTAAQKTPA 407 Query: 428 SHSDVAS 434 VA Sbjct: 408 REGPVAE 414 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 66.2 bits (160), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 94/393 (23%), Positives = 161/393 (40%), Gaps = 43/393 (10%) Query: 25 LHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDPYLLREK 84 LH + G+ L+ +TG+LL F +E+ L+P + Q QA + P + EK Sbjct: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQV----RQQATLTPQQVLEK 64 Query: 85 VAQAMGPQVHISQLVLHARPGRTVSFQADPAIDPATGKPYRLGYDEILVNPYTGVVQGVR 144 + Q PQ I+ + L A PG +V P G+ V+PYTG + Sbjct: 65 LRQEE-PQRRITTMTLSAEPGDSVRVVFAPPPGERRGQAR-------YVDPYTGAL---- 112 Query: 145 DRDKISLKPA---DLMPFLFKLHHSLALPRAPGALLLGTVGLLWTLDCFVGMILTWPRAR 201 L P + F + H L LP G ++LGT+ L + G+ L WPR Sbjct: 113 ------LPPVTGMEFFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRA 166 Query: 202 PFLVKW-KPAWLIKWRAGFYRINLDLHRAFGLWCWVMLLLFAWSSVMLNL---RDQIYLP 257 L W + + ++ R+ + +LH G W VM ++FA + L +D + Sbjct: 167 LSLRAWLRLDFQLQGRSLLW----NLHSVIGTWSLVMYVIFATTGAYWALDWFKDGV--- 219 Query: 258 VMSRILSFDTSWRP-GPPLPQALSGPPQSWPEAHALARRTMDGFARQRGLVIDFEEKLAF 316 L+ +T P + GP P A L + + F + + +L Sbjct: 220 ---NTLAGETRRAPMARGEGKKKDGPNNQTPVALDLT-QAWNAFLTKAPDYQSAQLRLPE 275 Query: 317 DRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFSNWLGALHMG 376 + + I+ + +A + + + + ++G+I G F + LHMG Sbjct: 276 QGTQPLQIFFLGHDAP--HERARSRMVVLPQSGEIKQLERYEDKSTGGRFIAAIYPLHMG 333 Query: 377 HVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKR 409 FGLP RI++ + + L +TG +++ +R Sbjct: 334 TYFGLPGRIIMAIASLMMPLFGITGWMLYLDRR 366 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.465 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,379,266 Number of extensions: 56329 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 3 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)