BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0173.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 668 0.0 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 156 2e-39 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 132 6e-32 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 105 4e-24 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 88 9e-19 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 668 bits (1723), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/342 (99%), Positives = 339/342 (99%) Query: 1 MPSNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMAS 60 MPSNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMAS Sbjct: 1 MPSNPIPLQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMAS 60 Query: 61 EEQFQRLVALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGL 120 EEQFQRLVALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGL Sbjct: 61 EEQFQRLVALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGL 120 Query: 121 FEALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGAD 180 FEALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGAD Sbjct: 121 FEALDGASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGAD 180 Query: 181 AQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGADPLVAQPGEI 240 AQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAG DPLVAQPGEI Sbjct: 181 AQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGVDPLVAQPGEI 240 Query: 241 VDIHADGRLVRRAARESAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADW 300 VDIH DGRLVRRAAR SAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADW Sbjct: 241 VDIHPDGRLVRRAARASAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADW 300 Query: 301 QVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVAAAGKR 342 QVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVAAAGKR Sbjct: 301 QVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVAAAGKR 342 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 156 bits (395), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 117/333 (35%), Positives = 169/333 (50%), Gaps = 22/333 (6%) Query: 8 LQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRL 67 + + +L +A WL+++ P DAA ++ +A WR P HE+ WS +E F L Sbjct: 11 IDEAILTEAATWLMEMHEGPLDAA----RRSKLAQWRQRSPEHERAWSRAALLQETFAAL 66 Query: 68 VALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGA 127 A + GP R + + + +G A G +A A++ + GA Sbjct: 67 PPGGATALKQVGPAGRRKAIKLLAYALT--LGPA----GWLAYHALSGPE-------QGA 113 Query: 128 SYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHR 187 AT G++R+++L DG+ L LNT + + L F +RLL L++GEI + T D Q P R Sbjct: 114 RIATAIGERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPP-R 172 Query: 188 PFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAG-ADPLVAQPGEIVDIHAD 246 PF V T L+ LGTRF VR+ ++ VL+G V + A A V G Sbjct: 173 PFLVATRQGSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRS 232 Query: 247 GRLVRRAARESAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADWQVSGVF 306 G R ++ AW L+ +M LA + +L RYRSG LQ DPA+A +VSGVF Sbjct: 233 GSAAPRPCDDTQ---AAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVF 289 Query: 307 QLQDTDVALEALAQALPLTIERRTRFWVRVAAA 339 L DTD L LAQ LP+ +++ TR+WVRV A Sbjct: 290 PLADTDRVLRLLAQTLPVRVQQLTRYWVRVLPA 322 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 132 bits (331), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 111/324 (34%), Positives = 157/324 (48%), Gaps = 22/324 (6%) Query: 12 VLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRLVALP 71 V ++A+ WL++L+ P +AP AWR+A P HE+ W + + + Q L A P Sbjct: 20 VAERALEWLLELQTQP----LAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQAL-ASP 74 Query: 72 VPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGASYAT 131 V A+ L RRQ ++ + + G G +A G + + A T Sbjct: 75 VNAALAQAALTPSGNRRQVIKT----LALLVFG-GGVAWGLTRSEPWQQWRA----DLRT 125 Query: 132 RTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHRPFWV 191 G QR+L L DG+ L LNT S+V L F R L L GE+ + TG RPF V Sbjct: 126 DIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAG---GGRPFLV 182 Query: 192 QTAHARLQALGTRFDVRQHGDGTQLTVLEG--RVAVHVAGADPLVAQPGEIVDIHADGRL 249 +TA ALGTR+ VRQ T+++V EG R+ A V Q G+ A G Sbjct: 183 ETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSAQGVG 242 Query: 250 VRRAARESAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADWQVSGVFQLQ 309 A E+ M AW + +V R MRL +A+L RY + L C PAV+ ++SG + + Sbjct: 243 DVTTADETRM---AWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVA 299 Query: 310 DTDVALEALAQALPLTIERRTRFW 333 D+D + LA AL L + R RFW Sbjct: 300 DSDAVIATLAAALGLQAQLRQRFW 323 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 105 bits (263), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 102/337 (30%), Positives = 151/337 (44%), Gaps = 37/337 (10%) Query: 10 DPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRLVA 69 + +++QAI W+++L GD + P +A W+AADPRH ++ L S +QF Sbjct: 5 EKIVEQAIEWMLQLEQ--GD--LPPGERAAFEQWKAADPRHAMAYANLSQSVQQFD---- 56 Query: 70 LPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGASY 129 VP P+ N +Q S+ RR+ + G A + AL A Sbjct: 57 --VPRQVGAAPVILRNTLQQKSK-RRKALKRIAALAGLAVGAAALLDRVTPVAALT-ADL 112 Query: 130 ATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHRPF 189 T TG +++ L DG+ + LN S++ R + L +GEI + A + PF Sbjct: 113 HTGTGQRQRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAA----ANALPF 168 Query: 190 WVQTAHARLQAL-GT--------RFDVRQHGDGTQLTVLEGRVAVHVAGADPLVAQPGEI 240 ++T H + A GT R D+ L+ G+ A AG Q EI Sbjct: 169 SIETVHGSVSATAGTLMLGLQPLRTDLVAQQAAALLSTRNGQHATLAAGQRTWFDQ-DEI 227 Query: 241 VDIHADGRLVRRAARESAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADW 300 A R + +W+E L +VVA L YR G L+ DPAVA Sbjct: 228 -----------GAPRAAGGAENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLDPAVAQL 276 Query: 301 QVSGVFQLQDTDVALEALAQALPLTIERRTRFWVRVA 337 ++SG F L DTD AL ALA ALP+T+ R + +WV ++ Sbjct: 277 RISGAFPLDDTDRALSALASALPITVNRVSPYWVSIS 313 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 88.2 bits (217), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 70/202 (34%), Positives = 103/202 (50%), Gaps = 11/202 (5%) Query: 127 ASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHH 186 A T TG L DG+ L L+ SS+ FD RR + L QGE+ ++ D Sbjct: 127 ADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPA---- 182 Query: 187 RPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGAD----PLVAQPGEIVD 242 RPF +QTA ++ALGTRF VR+ GT +++LE +V++ GAD PLV + G+ V Sbjct: 183 RPFVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQ-PGADTTQAPLVLEAGQRVR 241 Query: 243 IHADGRLVRRAARESAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPA-VADWQ 301 +H +A++ AW LV L +V+ +L R+R G + D A +A Sbjct: 242 LHGGAAGPLEHIDAAALE-RAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLH 300 Query: 302 VSGVFQLQDTDVALEALAQALP 323 ++ V L DT AL+ L +P Sbjct: 301 MTVVLPLDDTQRALQLLVDGVP 322 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,080,014 Number of extensions: 42882 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 6 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)