BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0171.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311750|ref|YP_003775842.1| transcription response regulato... 422 e-119 gi|300313680|ref|YP_003777772.1| two component response regulato... 226 2e-60 gi|300309981|ref|YP_003774073.1| two component response regulato... 168 3e-43 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 153 9e-39 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 147 7e-37 >gi|300311750|ref|YP_003775842.1| transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 422 bits (1085), Expect = e-119, Method: Compositional matrix adjust. Identities = 216/216 (100%), Positives = 216/216 (100%) Query: 1 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR 60 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR Sbjct: 5 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR 64 Query: 61 QLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAASD 120 QLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAASD Sbjct: 65 QLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAASD 124 Query: 121 QLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGEE 180 QLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGEE Sbjct: 125 QLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGEE 184 Query: 181 VESNAVEFLIHSLRRKLDKEAIKNVRGMGWLVQRAS 216 VESNAVEFLIHSLRRKLDKEAIKNVRGMGWLVQRAS Sbjct: 185 VESNAVEFLIHSLRRKLDKEAIKNVRGMGWLVQRAS 220 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 226 bits (575), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 119/213 (55%), Positives = 152/213 (71%), Gaps = 2/213 (0%) Query: 1 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR 60 LVEDD M+GE VR L+ +G+A DWV+ G +A +AL + Y LLLDLGLP DGLAVLR Sbjct: 5 LVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGLAVLR 64 Query: 61 QLRTH-SLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAAS 119 LR + +PVL+ TARD + DR+ GLDAGADDYL+KPF + EL AR+RA+ RR++G A Sbjct: 65 TLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQAGRAE 124 Query: 120 DQLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGE 179 + ++ L+ A G V LSAREF LL A M RPG +LSRA+LEEKLYGW + Sbjct: 125 SLVQVREVILNPATHEVTV-SGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLYGWDD 183 Query: 180 EVESNAVEFLIHSLRRKLDKEAIKNVRGMGWLV 212 +ESNAVE IH+LR+K+ + IKNVRG+G+LV Sbjct: 184 SIESNAVEVHIHALRKKVGSDFIKNVRGVGYLV 216 >gi|300309981|ref|YP_003774073.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 237 Score = 168 bits (425), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 94/217 (43%), Positives = 143/217 (65%), Gaps = 4/217 (1%) Query: 1 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR 60 L EDD+++ + + +L+ +GYAAD V+ G A AL+ Q +D L+LDLGLP GL VLR Sbjct: 5 LAEDDSVLADGLTRSLRHSGYAADCVKNGVEADSALSTQDFDLLILDLGLPKMSGLEVLR 64 Query: 61 QLRT-HSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAAS 119 +LR +S LPVL++TA D ++ R+ GLD GADDY+ KPFA+ EL AR+RA+ RR +G Sbjct: 65 RLRARNSRLPVLILTAADSVEQRVQGLDLGADDYMAKPFALSELEARVRALTRRGAGGGP 124 Query: 120 DQLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGE 179 + +G ++ D + A E + LSARE LL L+ R G ++S+ +L + L WGE Sbjct: 125 TVIRHGPLSYDQVGRIAYIGE-QMLELSARELGLLEVLLQRTGRLVSKEQLVDHLCEWGE 183 Query: 180 EVESNAVEFLIHSLRRKLDKEAIK--NVRGMGWLVQR 214 EV +NA+E +H LR+K++ ++ VRG+G+ +++ Sbjct: 184 EVSNNAIEVYVHRLRKKIEVGGVRIATVRGLGYCLEK 220 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 153 bits (387), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 85/215 (39%), Positives = 129/215 (60%), Gaps = 5/215 (2%) Query: 1 LVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGLAVLR 60 LVED+ G+ +R L ++G+ DWV+ G L Y ++LD+ LPG +G +LR Sbjct: 5 LVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGWDILR 64 Query: 61 QLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAASD 120 +LR PVL +TARD ++DR+ GL+ GADDYL+KPFA ELLAR+R +LRR D Sbjct: 65 ELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPSREVD 124 Query: 121 QLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGEE 180 + + LD+ + R G + L+A+EFALL L+ RPG +LSR ++ +++ + Sbjct: 125 LIEYDDVVLDVLRRKVT-RAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDMNFD 183 Query: 181 VESNAVEFLIHSLRRKLD----KEAIKNVRGMGWL 211 ++N V+ + LR KLD K+ + V GMG++ Sbjct: 184 SDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYV 218 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 147 bits (371), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 89/218 (40%), Positives = 132/218 (60%), Gaps = 7/218 (3%) Query: 1 LVEDDAMIGEEVRCALKDNGYAADWVRAG-DAALLALAQQQYDALLLDLGLPGADGLAVL 59 +VED+ + E + L + G A D G D LA+ + YD ++LD+ LPG DG +VL Sbjct: 5 IVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAI-EHDYDVIVLDVMLPGLDGFSVL 63 Query: 60 RQLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSGAAS 119 R LR PV+++TARD ++DRI GL GADDYL+KPF+ ELLARL+A+ RR Sbjct: 64 RALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRAQEP 123 Query: 120 DQLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYGWGE 179 QL G + +DL ++ A +R G + L+A+EFALL + R G ILS+ + E ++ Sbjct: 124 AQLRIGDLQIDLLSRKA-FRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDMNF 182 Query: 180 EVESNAVEFLIHSLRRKLD----KEAIKNVRGMGWLVQ 213 + +N VE I LR K+D ++ + VRGMG++++ Sbjct: 183 DSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLE 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 534,574 Number of extensions: 16407 Number of successful extensions: 159 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 36 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)