BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0170.p (439 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 857 0.0 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 173 3e-44 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 166 3e-42 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 166 5e-42 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 161 9e-41 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 857 bits (2213), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/439 (99%), Positives = 439/439 (100%) Query: 1 MKRSLQAQLSLALTAAILVVALGAGGFGFVTAYRDSIRSQDDLLMQVGALLKHSGAALSL 60 MKRSLQAQLSLALTAAILVVALGAGGFGFVTAYRDSIRSQDDLLMQVGALLKHSGAALSL Sbjct: 8 MKRSLQAQLSLALTAAILVVALGAGGFGFVTAYRDSIRSQDDLLMQVGALLKHSGAALSL 67 Query: 61 EGAGMAAAGDEHDSRVTVQWLPDPGDPPADAAPLPLPADLAEGFHTLLLHHEYFRVYVQA 120 EGAGMAAAGDEHDSRVTVQWLPDPGDPPADAAPLPLPADLAEGFHTLLLHHEYFRVYVQA Sbjct: 68 EGAGMAAAGDEHDSRVTVQWLPDPGDPPADAAPLPLPADLAEGFHTLLLHHEYFRVYVQA 127 Query: 121 LPNGKRLALAQETDLRQHIAQRSAWRALWPFVVLAPLLLLAVQRIIKRLFAPLQALRQEI 180 LPNGKRLALAQETDLRQHIAQRSAWRALWPFVVLAPLLLLAVQRIIKRLFAPLQALRQEI Sbjct: 128 LPNGKRLALAQETDLRQHIAQRSAWRALWPFVVLAPLLLLAVQRIIKRLFAPLQALRQEI 187 Query: 181 DSRGEDDLQPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHELRTPLTALS 240 DSRGEDDLQPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHELRTPLTALS Sbjct: 188 DSRGEDDLQPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHELRTPLTALS 247 Query: 241 LQAERLGQAPMSETARERLTRLETGLQRARQLVTQLLALARAQTPAGAALQPCSLHQVLR 300 LQAERLG+APMSETARERLTRLETGLQRARQLVTQLLALARAQTPAGAALQPCSLHQVLR Sbjct: 248 LQAERLGRAPMSETARERLTRLETGLQRARQLVTQLLALARAQTPAGAALQPCSLHQVLR 307 Query: 301 EVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNLIDNAIAYTPAGGAVSLRME 360 EVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNLIDNAIAYTPAGGAVSLRME Sbjct: 308 EVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNLIDNAIAYTPAGGAVSLRME 367 Query: 361 RHAQWVALEICDTGPGIAASERERVFQPFYRIDPSGQGSGLGLAIVQSIARRLGLTLRLE 420 RHAQWVALEICDTGPGIAASERERVFQPFYRIDPSGQGSGLGLAIVQSIARRLG+TLRLE Sbjct: 368 RHAQWVALEICDTGPGIAASERERVFQPFYRIDPSGQGSGLGLAIVQSIARRLGVTLRLE 427 Query: 421 AADPAQQSGLRVRLLIPGA 439 AADPAQQSGLRVRLLIPGA Sbjct: 428 AADPAQQSGLRVRLLIPGA 446 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 173 bits (438), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 130/347 (37%), Positives = 191/347 (55%), Gaps = 35/347 (10%) Query: 116 VYVQALPNGKRLALAQETDLRQHIAQRSAWRALW---PFVVLAPLLLLAVQRIIKRLFAP 172 VY ++ + + + + R + ++ + LW V +A LL+ R + R P Sbjct: 144 VYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAALLVWFAVRFVLR---P 200 Query: 173 LQALRQEIDSRGEDDLQPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHEL 232 L L+ E++SR DL L+ +E++ V A+NG + R++A ++AQRRF+ADA+H+L Sbjct: 201 LMQLKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALISAQRRFIADASHQL 260 Query: 233 RTPLTALSLQAERLGQAPMSETARER-----LTRLETGLQR----ARQLVTQLLALARAQ 283 RTPLT L Q+E + E RE+ L L G+ R A L +LL LARA+ Sbjct: 261 RTPLTVLKTQSE----LTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRLLTLARAE 316 Query: 284 TPAG-AALQPCSLHQVLREVIEELLPLAQCKQIDLGLDGERDACVLTQPQELAQLLRNLI 342 A P SL R+V EL A ++I L + E D + L +L+ NL+ Sbjct: 317 HGAAEGGTMPLSLSDAARQVALELSQQAVSREIGLAFEEEGDVSIEANALLLHELMVNLL 376 Query: 343 DNAIAYTPAGGAVSLRMERHAQ----WVA-LEICDTGPGIAASERERVFQPFYR------ 391 DNAI YTPAGG V+LR+ + VA LE+ D+GPGI A+ERE+VF+PFYR Sbjct: 377 DNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPFYRAGATQH 436 Query: 392 IDPSGQGSGLGLAIVQSIARRLGLTLRLEAADPAQQSGLRVRLLIPG 438 ++P G+GLGL+IV+ IA ++ L+A PAQ SGL VR++ G Sbjct: 437 VNPG--GTGLGLSIVREIAAMHRASVTLDA--PAQGSGLVVRIVFQG 479 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 166 bits (421), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 116/316 (36%), Positives = 173/316 (54%), Gaps = 17/316 (5%) Query: 126 RLALAQ--ET-DLRQHIAQRSAWRALWPFVVLAPLLLLAVQRIIKRLFAPLQALRQEIDS 182 RLA Q ET + R +A + P ++ P+ L V + R +PL L+Q I + Sbjct: 51 RLATVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRA 110 Query: 183 RGEDDLQPIAERLLPREVQAFVAAINGLLARVEAGMAAQRRFVADAAHELRTPLTALSLQ 242 R DDL PI +P E+ V ++N +LAR+ +A Q+RF+ADAAH+++TPL + +Q Sbjct: 111 RRPDDLSPIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQ 170 Query: 243 AERLGQAPMSETARERLTRLETGLQRARQLVTQLLALARA--QTPAGAALQPCSLHQVLR 300 +E + R L +L + A +LV QLL+LARA Q + P L+++ R Sbjct: 171 SELALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELAR 230 Query: 301 EVIEELLPLAQCKQIDLGLDGERDACVLT-QPQELAQLLRNLIDNAIAYTPAGG----AV 355 V+++ +P++ ++IDLG + ++T P L +LL NL+DNA+ YTP G V Sbjct: 231 SVVQDWVPMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTV 290 Query: 356 SLRMERHAQWVALEICDTGPGIAASERERVFQPFYRI--DPS-GQGSGLGLAIVQSIARR 412 R + LE+ D GPGI +ER VF+ FYRI PS G G GLGLAIV+ IA++ Sbjct: 291 RARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQ 350 Query: 413 ----LGLTLRLEAADP 424 L +T + DP Sbjct: 351 HEAALEITSNPRSTDP 366 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 166 bits (419), Expect = 5e-42, Method: Compositional matrix adjust. Identities = 135/359 (37%), Positives = 189/359 (52%), Gaps = 25/359 (6%) Query: 94 LPLPADLAEGFHTLLLHHEYFRVYVQALPNGKRLALAQETDLRQHIAQRSAWRALWPFVV 153 +P A+L GF + + +RVY A + +AQ R IA + A + + P ++ Sbjct: 92 MPRQAEL--GFANVSGPNGMWRVY-SAQIGSTVVQVAQPQSARDEIAAQMAVKTVVPLLL 148 Query: 154 LAPLLLLAVQRIIKRLFAPLQALRQEIDSRGEDDLQPIAERLLPREVQAFVAAINGLLAR 213 L PL+ + + R AP++ E+ +R + LQP+A+ +P+E+Q A+N LLAR Sbjct: 149 LFPLIGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADVGMPQEIQPLTHALNDLLAR 208 Query: 214 VEAGMAAQRRFVADAAHELRTPLTALSLQAERLGQAPMSETARERLTRLETGLQRARQLV 273 ++ QR FVADAAHELRTPLTAL LQ + +A E R L+ GL+R LV Sbjct: 209 LKHATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNGLERTTHLV 268 Query: 274 TQLLALARAQTPAGAALQPCSLHQVLREVIEELLPLAQCKQIDLGL----DGERDACVLT 329 QLL LAR Q P + L +L V+ +L +A+ IDLGL D R V Sbjct: 269 HQLLTLAR-QEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTG 327 Query: 330 QPQELAQLLRNLIDNAIAYTPAGGAVSLRME-RHAQWVALEICDTGPGIAASERERVFQP 388 L L NL+DNA+ YTPAGG V + ++ A + + + D+GPGIA SE RVF Sbjct: 328 NADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDR 387 Query: 389 FYRIDPSGQ--------GSGLGLAIVQSIA--RRLGLTLRLEAADPAQQSGLRVRLLIP 437 FYR S + GSGLGLAIV+ IA R+ + LR GL+ R++ P Sbjct: 388 FYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVELRNTG------HGLQARVVFP 440 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 161 bits (408), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 113/288 (39%), Positives = 163/288 (56%), Gaps = 9/288 (3%) Query: 157 LLLLAVQRIIKRL---FAPLQALRQEIDSRGEDDLQPIAERLLPREVQAFVAAINGLLAR 213 LLLLA+ ++ L PL AL+ E+ +R D L P+ L +E++ V AIN + R Sbjct: 170 LLLLAISLVVLGLTMELRPLLALKDELATRRPDSLTPLRAGGLQQELRPIVEAINQYIFR 229 Query: 214 VEAGMAAQRRFVADAAHELRTPLTALSLQAERLGQAPMSETARERLTRLETGLQRARQLV 273 + A + Q+RFVADAAH+LRTPL + Q + Q + L L+ G + L Sbjct: 230 LNAQVTVQKRFVADAAHQLRTPLAVIDSQIQFARQLDDPARVAQVLASLQEGSRSMTDLT 289 Query: 274 TQLLALARAQTPAGAAL--QPCSLHQVLREVIEELLPLAQCKQIDLGLDGERDACVLTQP 331 +LL L++A+ +A P L V V+EEL LA K IDLGL+ ++ ++ Sbjct: 290 NKLLLLSQAEASDSSAFPRAPVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAMVNGN 349 Query: 332 Q-ELAQLLRNLIDNAIAYTPAGGAVSLRMERHAQWVALEICDTGPGIAASERERVFQPFY 390 + +L +L NLIDNA+ YTP GG V++ +E+ + + + D GPGI A RERVF+ FY Sbjct: 350 EAQLMGMLMNLIDNALRYTPEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFY 409 Query: 391 R-IDPSGQGSGLGLAIVQSIARRLGLTLRLEAADPAQQSGLRVRLLIP 437 R P GSGLGLAIV+ I + ++RL+A PAQ SGL + +P Sbjct: 410 RNAAPDQPGSGLGLAIVREIVQASQGSIRLDA--PAQGSGLVATVSLP 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,194,646 Number of extensions: 48575 Number of successful extensions: 352 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 44 Length of query: 439 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 351 Effective length of database: 1,203,190 Effective search space: 422319690 Effective search space used: 422319690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)