BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0166.p (414 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 814 0.0 gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 115 7e-27 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 65 7e-12 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 65 1e-11 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 64 2e-11 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 814 bits (2103), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/414 (99%), Positives = 414/414 (100%) Query: 1 MSPIAPPSPQHPPNHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVT 60 ++PIAPPSPQHPPNHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVT Sbjct: 4 IAPIAPPSPQHPPNHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVT 63 Query: 61 GYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGI 120 GYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGI Sbjct: 64 GYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGI 123 Query: 121 AATNLLAKAIITDSFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLV 180 AATNLLAKAIITDSFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLV Sbjct: 124 AATNLLAKAIITDSFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLV 183 Query: 181 YSLGMWAVLWRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIV 240 YSLGMWAVLWRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIV Sbjct: 184 YSLGMWAVLWRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIV 243 Query: 241 GPFMVQNTLHQPPTFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAF 300 GPFMVQNTLHQPPTFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAF Sbjct: 244 GPFMVQNTLHQPPTFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAF 303 Query: 301 VMLLLALSVGLTVWTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLV 360 VMLLLALSVGLTVWTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLV Sbjct: 304 VMLLLALSVGLTVWTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLV 363 Query: 361 SAAMMGLAGFVSIHSLTPLAGFFCVVGLTVIVMVGKLLHHLQVPAPLARPVARP 414 SAAMMGLAGFVSIHSLTPLAGFFCVVGLTVIVMVGKLLHHLQVPAPLA+PVARP Sbjct: 364 SAAMMGLAGFVSIHSLTPLAGFFCVVGLTVIVMVGKLLHHLQVPAPLAQPVARP 417 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 115 bits (288), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 75/252 (29%), Positives = 126/252 (50%), Gaps = 5/252 (1%) Query: 20 LFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADA 79 L ++ + G LAID+Y+P+ +A D +Q ++ +LI A GQ+ +GPL+D Sbjct: 17 LAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDR 76 Query: 80 YGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQA 139 +GRK L G+ L+ + +L + A N+ET + R+ QG G +A ++ +A++ D + Sbjct: 77 FGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 Query: 140 LVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYRETLAQP 199 A++ + + +GLAPI+AP IG + WR V L ++ L +Y Sbjct: 137 SAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVDV 196 Query: 200 VRLHPATLARNARM---VLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPPTFF 256 R P L R R +L F S L GL + + + GPF+V P +F Sbjct: 197 SRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQYF 256 Query: 257 GYL--ALAIGMM 266 G++ + AIG++ Sbjct: 257 GFVFASNAIGLI 268 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 54/171 (31%), Positives = 78/171 (45%), Gaps = 17/171 (9%) Query: 41 LPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVLS 100 LP +A D S I+ V+ Y++ +A L G AD YGR+R+L G+ LF+L + Sbjct: 59 LPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLAC 118 Query: 101 LAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQALVHAYTYMAIAW-------GL 153 AP+ + AR LQG G A A A+I +F H AW GL Sbjct: 119 ALAPSAQALYGARALQGVGAAFLLAPALAVIGHAF------HDEDQRERAWALWGGIMGL 172 Query: 154 APIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRY----RETLAQPV 200 ++AP+IG + GWR L L + +WR+ R L +P+ Sbjct: 173 TMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPRPL 223 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 40/133 (30%), Positives = 68/133 (51%) Query: 40 ALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVL 99 +LP +A D ++ ++T Y+ A + G +AD G +++ + + +F G +L Sbjct: 9 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 68 Query: 100 SLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQALVHAYTYMAIAWGLAPIIAP 159 + +L TFVAAR LQG G A + + II S LV A +Y+ + L P++ P Sbjct: 69 CAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 128 Query: 160 VIGAHLQEAFGWR 172 ++G + F WR Sbjct: 129 LLGGFITTYFHWR 141 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 41/133 (30%), Positives = 67/133 (50%) Query: 40 ALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADAYGRKRILALGLGLFSLGCVL 99 +LP +A+D +++ ++T YL++ A + G +AD +G I + +F LG +L Sbjct: 9 SLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAICVFMLGSIL 68 Query: 100 SLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQALVHAYTYMAIAWGLAPIIAP 159 + L FVAAR LQG G A + + +I S LV A +Y+ I P++ P Sbjct: 69 CGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGP 128 Query: 160 VIGAHLQEAFGWR 172 +G + F WR Sbjct: 129 PLGGFITTYFHWR 141 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.141 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,212,594 Number of extensions: 52030 Number of successful extensions: 364 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 363 Number of HSP's successfully gapped: 18 Length of query: 414 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 326 Effective length of database: 1,203,190 Effective search space: 392239940 Effective search space used: 392239940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)