BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0160.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 348 4e-97 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 336 2e-93 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 232 4e-62 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 134 1e-32 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 124 9e-30 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 348 bits (893), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 181/333 (54%), Positives = 235/333 (70%), Gaps = 4/333 (1%) Query: 1 MGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWEDLKPSRIFSRAA 60 MGTASTM S+VEA+G LP +IPA+DA + +G RIV MV EDL S+I +R A Sbjct: 202 MGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMVEEDLVLSKILTRQA 261 Query: 61 VDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPSGTRLMED 120 +N I A+GGSTNA IH +AIAGR G++L L D+ +G +P L D+ PSG LMED Sbjct: 262 FENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLMED 321 Query: 121 FYYAGGLPALLEQLKPHLALQSLTVTGLTLGENLARCAASARNAKETAIRPLDQPVTDGA 180 FYYAGGLPA++ QL+ + +LT G TL EN A N E IR D+P + A Sbjct: 322 FYYAGGLPAVIRQLESVIDKTALTANGKTLWEN----CQDAPNWNEEVIRSFDKPFKEAA 377 Query: 181 ALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALVFDSIAEMNATIHAPDLDVDADTVLVL 240 +A+L+GNLAP+GAVIKPSAA+P LLKHRGRA+VF++ +++ I +LDVD VLVL Sbjct: 378 GIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVL 437 Query: 241 RGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDARMSGTHYGTCVLHVAPESAVGGP 300 + GP G PGM E GN+P+P K+L G+ DM+R+SDARMSGT YGT VLHV+PE+A GGP Sbjct: 438 KNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGP 497 Query: 301 LALIRTGDIIELDVAAGSINVLLSDEELAQRRS 333 LA+++ GD IELDV A +++ +SDEELA+RR+ Sbjct: 498 LAVVQNGDFIELDVEARKLHLDVSDEELARRRA 530 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 336 bits (862), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 176/344 (51%), Positives = 233/344 (67%), Gaps = 11/344 (3%) Query: 1 MGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWEDLKPSRIFSRAA 60 MGTASTM + E++G +LP +IPA+DA + SG RIV MVWE L S+I +R A Sbjct: 204 MGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIVDMVWEGLTLSKILTRKA 263 Query: 61 VDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPSGTRLMED 120 +N I A+GGSTNA IH AIAGR GV+L L+D+ ++G G P + D+ PSG LME+ Sbjct: 264 FENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRYLMEE 323 Query: 121 FYYAGGLPALLEQLK-----PHLALQSLTVTGLTLGENLARCAASARNAKETAIRPLDQP 175 FYYAGGLPA+L +L PH +LTV G T+ +N+ A + +RPL +P Sbjct: 324 FYYAGGLPAVLRRLGEADLLPHK--DALTVNGQTMWDNVK----DAPIYNDEVVRPLAKP 377 Query: 176 VTDGAALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALVFDSIAEMNATIHAPDLDVDAD 235 + + + +LRGNLAP GAV+KPSAA+P L+KHRGRA+VF+ I+ PDLDVDA Sbjct: 378 LIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDAS 437 Query: 236 TVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDARMSGTHYGTCVLHVAPES 295 VLV++ GP G PGM E GN+ +P K+LA GV+DM+RISDARMSGT YGT +LHVAPE+ Sbjct: 438 CVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEA 497 Query: 296 AVGGPLALIRTGDIIELDVAAGSINVLLSDEELAQRRSPREVVV 339 A GGPL +++ GD IELD AG + + +S+EE+ +R R V+ Sbjct: 498 AAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVL 541 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 232 bits (591), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 137/346 (39%), Positives = 204/346 (58%), Gaps = 18/346 (5%) Query: 1 MGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWEDLKPSRIFSRAA 60 MGTASTM ++ EA+G +L G ++IPA +M +G RIV M +EDL+PS I +R A Sbjct: 206 MGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMAYEDLRPSAILTRDA 265 Query: 61 VDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPSGTRLMED 120 + I+ A+GGSTNA H +A+A GVEL +D++K G +P+L ++ P+G L E Sbjct: 266 FLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYDVPLLLNMQPAGKYLGER 325 Query: 121 FYYAGGLPALLEQLKP--HLALQSLTVTGLTLGENLARCAASARNAKETAIRPLDQPVTD 178 F+ AGG+PA++ +L+ L + +T TG T+ ENL A++ R I P P+ + Sbjct: 326 FHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDRE----MIYPFAAPLRE 381 Query: 179 GAALAVLRGNLAPNGAVIKPSAASPHLLKH-----------RGRALVFDSIAEMNATIHA 227 A VL+GNL + A++K S S + RA+VFD + +A I+ Sbjct: 382 RAGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDYHARIND 440 Query: 228 PDLDVDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDARMSGTHYGTC 287 P L +D +T+L +RG GP+G PG E N+ P L+ GV + + D R SGT Sbjct: 441 PALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDGRQSGTSDSPS 500 Query: 288 VLHVAPESAVGGPLALIRTGDIIELDVAAGSINVLLSDEELAQRRS 333 +L+ +PESAVGG LA +R GD + +D+ G N+L+S+EELA+R+S Sbjct: 501 ILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRKS 546 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 107/382 (28%), Positives = 173/382 (45%), Gaps = 52/382 (13%) Query: 1 MGTASTMTSIVEAMGFTLPGATSIPAMDAAHPRMCAASGERIVAMVWEDLK-------PS 53 M TA++M + E +G +LPG +I A A + +G IV + + P Sbjct: 202 MFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPR 261 Query: 54 RIFSRAAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPS 113 I ++ +N + +++GGSTN +H +A A VE T+ D ++ +P L + P Sbjct: 262 NICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPM 321 Query: 114 GTRL-MEDFYYAGGLPALLEQLKPHLALQSL--TVTGLTLGENLA--------------- 155 + +ED + AGG+ ++L +L L + TV TLGE + Sbjct: 322 TNKYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKL 381 Query: 156 ------------------RCAASARNAKETAIRPLDQPVTDGAALAVLRGNLAPNGAVIK 197 R A+ + IR + + LAVL GN+A G ++K Sbjct: 382 FRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVK 441 Query: 198 PSAASPHLLKHRGRALVFDSIAEMNATIHAPDLD-VDADTVLVLRGGGPIGAPGMPE--W 254 + +LK G A VF+S +A + D V A V+++R GP G PGM E + Sbjct: 442 TAGVDESILKFSGTARVFES---QDAAVEGILGDKVKAGDVVIVRYEGPKGGPGMQEMLY 498 Query: 255 GNLPIPKKLLAAGVRDMLRISDARMSGTHYGTCVLHVAPESAVGGPLALIRTGDIIELDV 314 I K L +D R SG G + H +PE+A GG + L+ GD+I++D+ Sbjct: 499 PTSYIKSKGLGKAC---ALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDI 555 Query: 315 AAGSINVLLSDEELAQRRSPRE 336 +IN+ +S ++LA+RR+ E Sbjct: 556 PNRTINLRISSDDLAKRRTAME 577 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 124 bits (312), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 114/354 (32%), Positives = 162/354 (45%), Gaps = 30/354 (8%) Query: 2 GTASTMTSIVEAMGFTLPGATSI----PAMDAAHPRMCAASGERIVAMVWEDLKPSRIFS 57 GTA++ ++EAMG LPGA I P DA A +I + + R+ Sbjct: 227 GTANSNQMLMEAMGLHLPGAAFITPNTPLRDAL-TAAAAKQAAKITDLGTQYTPVGRMID 285 Query: 58 RAAVDNGIIAYMALGGSTNAAIHTIAIAGRGGVELTLDDFVKVGAGIPVLADIFPSGTRL 117 ++ N I+A A GGSTN +H +AIA G+ + DDF K+ A +P++ I+P+GT Sbjct: 286 EKSIVNAIVALHATGGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTAD 345 Query: 118 MEDFYYAGGLPALLEQLKP----HLALQSLTVTGLTL----------GEN-----LARCA 158 + F+ AGG +L +L H + ++ GL GEN + Sbjct: 346 VNHFHAAGGTGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHD 405 Query: 159 ASARNAKETAIRPLDQPVTDGAALAVLRGNLAPNGAVIKPSAASPHLLKHRGRALVFDSI 218 A A++ E + + +P L +L+GNL AVIK SA P A+VF S Sbjct: 406 APAQSGDEGVLGTVAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQ 463 Query: 219 AEMNATIHAPDLDVDADTVLVLRGGGPIGAPGMPEWGNLPIPKKLLAAGVRDMLRISDAR 278 A A LD D V+ +G A GMPE L +L R + ++D R Sbjct: 464 EAFMAAFKAGQLDRDFVAVITFQG---PRANGMPELHALTPALGILQDKGRHVALVTDGR 520 Query: 279 MSGTHYGT-CVLHVAPESAVGGPLALIRTGDIIELDVAAGSINVLLSDEELAQR 331 MSG +HV PE GGPL +R GDII LD AG++ + E A R Sbjct: 521 MSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,125,440 Number of extensions: 50196 Number of successful extensions: 196 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 6 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)