BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0157.p (135 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 248 2e-67 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 166 4e-43 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 62 2e-11 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 248 bits (632), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 118/121 (97%), Positives = 119/121 (98%) Query: 1 MKTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPASPHLRILPLDVADAHSIQ 60 MKTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPASPHLRILPLDVADAHSIQ Sbjct: 1 MKTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPASPHLRILPLDVADAHSIQ 60 Query: 61 AAVEAAGPVDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRGNGRG 120 AAVEAAGPVDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMR G+G Sbjct: 61 AAVEAAGPVDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRARGQG 120 Query: 121 L 121 L Sbjct: 121 L 121 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 166 bits (421), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 80/121 (66%), Positives = 93/121 (76%) Query: 1 MKTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLPASPHLRILPLDVADAHSIQ 60 M T+ ITGCSSG+GLE AR+FL WKVIATMR+PR DLL S +L ILPLDV+ SI+ Sbjct: 1 MNTVLITGCSSGYGLETARHFLANGWKVIATMRSPRFDLLSPSDNLCILPLDVSQPDSIK 60 Query: 61 AAVEAAGPVDVLVNNAGIGNLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQMRGNGRG 120 A+EAAGP+DVLVNNAGIG GA EA PM+ VRE+FETNT G +AM QA IPQ R G G Sbjct: 61 QALEAAGPIDVLVNNAGIGLFGAFEATPMSTVREIFETNTFGVMAMCQAVIPQFRERGDG 120 Query: 121 L 121 + Sbjct: 121 V 121 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 61.6 bits (148), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 44/126 (34%), Positives = 62/126 (49%), Gaps = 9/126 (7%) Query: 4 IFITGCSSGFGLEIARYFLQRDWKVIATMRTPR--HDLLPASPHLRILP--LDVADAHSI 59 + ITG ++GFG +AR F KVIA R DL+ ++LP LDV SI Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKSSI 62 Query: 60 QAAVE----AAGPVDVLVNNAGIG-NLGALEAQPMAQVRELFETNTLGSIAMTQAFIPQM 114 + + A VDVL+NNAG+ LG ++ + +TN G + +T A +P M Sbjct: 63 ETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPDM 122 Query: 115 RGNGRG 120 GRG Sbjct: 123 VKRGRG 128 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 425,662 Number of extensions: 17409 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 128 Number of HSP's successfully gapped: 6 Length of query: 135 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 59 Effective length of database: 1,260,010 Effective search space: 74340590 Effective search space used: 74340590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)