BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0151.p (448 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 894 0.0 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 198 6e-52 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 191 1e-49 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 187 2e-48 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 135 6e-33 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 894 bits (2311), Expect = 0.0, Method: Compositional matrix adjust. Identities = 442/448 (98%), Positives = 443/448 (98%) Query: 1 MSDSASSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWV 60 MS SASSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWV Sbjct: 1 MSGSASSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWV 60 Query: 61 NLFGHTNARINAALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGAS 120 NLFGHTN RINAALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGAS Sbjct: 61 NLFGHTNTRINAALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGAS 120 Query: 121 AVEIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHV 180 AVEIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHV Sbjct: 121 AVEIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHV 180 Query: 181 AASPDARQAAPGESAEEVAARALDDVESILQQREGRIAALIVEPLVQCAAGMAMHDPGYV 240 AASPDARQAAPGESAEEVAARALDDVE ILQQR+GRIAALIVEPLVQCAAGMAMHDPGYV Sbjct: 181 AASPDARQAAPGESAEEVAARALDDVERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYV 240 Query: 241 RGLRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMS 300 RGLRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMS Sbjct: 241 RGLRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMS 300 Query: 301 SEAIYQAFYDDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAAL 360 SEAIYQAFYDDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAAL Sbjct: 301 SEAIYQAFYDDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAAL 360 Query: 361 QPLAQDPRVRHVRQRGMIWACDAVLEGSAAAATAFSRRMAAEAAQREVLVRPIGASLYLM 420 QPLAQDPRVRHVRQRGMIWA DAVLEGSAAA TAFSRRMAAEAAQREVLVRPIGASLYLM Sbjct: 361 QPLAQDPRVRHVRQRGMIWAYDAVLEGSAAATTAFSRRMAAEAAQREVLVRPIGASLYLM 420 Query: 421 PPYLLDEEQAHWLGRQLHAAFAATMEAL 448 PPYLLDEEQAHWLGRQLHAAFAATMEAL Sbjct: 421 PPYLLDEEQAHWLGRQLHAAFAATMEAL 448 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 198 bits (504), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 145/439 (33%), Positives = 219/439 (49%), Gaps = 24/439 (5%) Query: 25 RRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGHTNARINAALVEQLGQLEHA 84 R S P+A+ G+ L D DG+RY+DA V+ GH + R+ A+ +Q+G+L +A Sbjct: 7 RNPRSKLPMAVA-GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYA 65 Query: 85 MLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHAWRNGGQPDKNEF 144 + FT P L+ LA +L H ++ S G+ AVE ALK++ + GQP + Sbjct: 66 HTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHI 125 Query: 145 ICIAGGYHGETVGALGVTDTPLFRSAYESLLRPA-HVAASPDARQAAPGESAEEVAARAL 203 I YHG T+GAL + R + +L A HV+ R A GES + R Sbjct: 126 IARRQSYHGNTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLA 185 Query: 204 DDVE-SILQQREGRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDRYGAHLIADEIAVG 262 D++E IL ++ A + E +V AG Y R +R++CD+YG LI DE+ G Sbjct: 186 DELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSG 245 Query: 263 CGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFYDDDVRRG---FLH 319 GRTG FACE+ GV PD++ ++KG+ GY P+ ++ S+ IY D V RG F H Sbjct: 246 MGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIY-----DAVLRGSGFFQH 300 Query: 320 SHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAAL-QPLAQDPRVRHVRQRGMI 378 H+Y G+ AC AA+A E+ +LE R L + L Q V +R RG+ Sbjct: 301 GHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLF 360 Query: 379 WACDAVLEGSAAAATA----FSRRMAAEAAQREVLVRPIGAS--------LYLMPPYLLD 426 + V E S+ + R+ AEA +R +LV P+G + + L PP++ Sbjct: 361 VGVELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIAS 420 Query: 427 EEQAHWLGRQLHAAFAATM 445 + ++L A AA++ Sbjct: 421 SNDISEIVQRLSDAVAASV 439 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 191 bits (484), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 135/442 (30%), Positives = 215/442 (48%), Gaps = 17/442 (3%) Query: 13 SLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGHTNARINA 72 SL + W P T R ++AP + ++ A G+ D G LD + W GH + +I + Sbjct: 11 SLSAYWMPFTANRNFKAAPRMLVS-AEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVS 69 Query: 73 ALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHA 132 A+ + + L++A H L+E+L A TG G+ FY G+ AV+ ALK+++ Sbjct: 70 AVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAY 129 Query: 133 WRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPA--HVAASPDARQAA 190 R G + I GYHG G + V R + + L P H+ + + + A Sbjct: 130 HRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNA 189 Query: 191 PGESAEEVAARALDDVESILQQRE-GRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDR 249 + E A D++E ++ + IAA+IVEP V + G+ + GY++ LR +C + Sbjct: 190 FTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP-VAGSTGVLIPPKGYLKRLREICTK 248 Query: 250 YGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFY 309 +G LI DE+ G GR G+ FA + V PD+ +KG++ G +P+ V I+ AF Sbjct: 249 HGILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFM 308 Query: 310 DDDVRRGFLHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQPLAQDPRV 369 + H ++Y+G+PLAC A LA+L +F + VLE +A A A L P V Sbjct: 309 NGPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLENAQAVADYFQEAAHALRGLPHV 368 Query: 370 RHVRQRGMIWACD-AVLEG--SAAAATAFSRRMAAEAAQREVLVRPIGASLYLMPPYLLD 426 VR G+I + A + G A A AF R A +L+R G + L PP +++ Sbjct: 369 IDVRTIGLIAGIELASIPGRVGARAYDAFVR-----AFNDGILIRVTGDIIALSPPLIIN 423 Query: 427 EEQAHWLGRQLHAAFAATMEAL 448 ++Q +L AA ++ L Sbjct: 424 KQQI----DELFGKLAAILKTL 441 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 127/429 (29%), Positives = 206/429 (48%), Gaps = 15/429 (3%) Query: 6 SSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGH 65 +SAL A R + HP T +R HE P+ + G +L D ++ LDA S W G+ Sbjct: 3 TSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGY 62 Query: 66 TNARINAALVEQLGQLEHAMLAGFTH---EPVVALSEQLAALTGQALGHCFYASDGASAV 122 + A EQ+ +L +A G+ H EP + L+++L ++ +L H ++ G+ AV Sbjct: 63 GQESVIRAATEQMQRLPYA--TGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV 120 Query: 123 EIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHVAA 182 + A++ H + G+P K FI + GYHG + G+T P F + L H Sbjct: 121 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP 180 Query: 183 SP-DARQAAPGESAEEVAA-RALDDVESILQQREGRIAALIVEPLVQCAAGMAMHDPGYV 240 SP R G+ A A+ +AL D + + +AA EP +Q + G+ + G++ Sbjct: 181 SPYPYRSELQGDEAIIAASVQALRD--KVAELGADNVAAFFCEP-IQGSGGVVVPPKGWL 237 Query: 241 RGLRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMS 300 + ++ C+ + DE+ G GRTG FAC V PDL+ ++KG++ GY P+ VM Sbjct: 238 KAMQLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMM 297 Query: 301 SEAIYQAFYDDDVRRGFL-HSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAA 359 S+ +Y D + H +Y+ +P++ AL L ++ E +L+ R PV A Sbjct: 298 SDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGG 357 Query: 360 LQPLAQDPRVRHVRQRGMIWACDAVLEGSAAAATAFSRRM---AAEAAQREVLV-RPIGA 415 L+ L P V R RG++ A + V + A A ++ +AA R LV R G Sbjct: 358 LRALLAHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGD 417 Query: 416 SLYLMPPYL 424 ++ P L Sbjct: 418 NILGFAPAL 426 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 135 bits (341), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 116/395 (29%), Positives = 181/395 (45%), Gaps = 48/395 (12%) Query: 37 RAHGLWLHDSDGRRYLDAISSWWVNLFGHTNARINAALVEQLGQLEHAMLAGFTHEPVVA 96 RA L D +GRR++D + V GH + ++ A+ Q+ + H + V Sbjct: 32 RAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVE 91 Query: 97 LSEQLAALT-GQALGHCFYASDGASAVEIALKMS-VHAWRNGGQPDKNEFICIAGGYHGE 154 L+E++ LT G + S GA AVE A+K++ H R G I AGG+HG Sbjct: 92 LAERINRLTPGNYPKKTAFFSTGAEAVENAIKIARAHTGRPG-------VIAFAGGFHGR 144 Query: 155 TVGALGVTDT-------------PLFRSAYESLLRPAHVAASPDARQAAPGESAEEVAAR 201 T+ + +T +F + Y S L H S DA +A G ++ A+ Sbjct: 145 TMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSAL---HGITSEDALEAVKGLFKSDIEAK 201 Query: 202 ALDDVESILQQREGRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDRYGAHLIADEIAV 261 R+AA+I+EP VQ G ++RGLR+LCD +G LIADE+ Sbjct: 202 --------------RVAAIILEP-VQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQS 246 Query: 262 GCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFYDDDVRRGFLHSH 321 G GRTG FA E V PDL+ ++K ++GG +PLS V I A + Sbjct: 247 GYGRTGKLFAMEHYDVLPDLMTMAKSLAGG-MPLSAVNGRAEIMDAPAPGGL------GG 299 Query: 322 SYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQPL-AQDPRVRHVRQRGMIWA 380 +Y GNPLA +ALA L + E+ ++ R + L L+ L + P++ VR G + A Sbjct: 300 TYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVA 359 Query: 381 CDAVLEGSAAAATAFSRRMAAEAAQREVLVRPIGA 415 + + +++++ A +L+ G+ Sbjct: 360 VEFADPATGKPDAEYTKKVQQHALNNGLLLLTCGS 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,373,824 Number of extensions: 56132 Number of successful extensions: 238 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 10 Length of query: 448 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 360 Effective length of database: 1,203,190 Effective search space: 433148400 Effective search space used: 433148400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)