BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0148.p (552 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 1121 0.0 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 327 2e-90 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 284 1e-77 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 280 2e-76 gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 110 3e-25 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 1121 bits (2899), Expect = 0.0, Method: Compositional matrix adjust. Identities = 550/552 (99%), Positives = 551/552 (99%) Query: 1 MPSSSSSPTNPSQAPAQSASDRYTDAGVRNDLAKVAVGNDINDIFFAAVEMTRMPMIVSD 60 MPSSSSSPTNPSQAPAQ ASDRYTDAGVRNDLAKVAVGNDINDIFFAAVEMTRMPMIVSD Sbjct: 1 MPSSSSSPTNPSQAPAQGASDRYTDAGVRNDLAKVAVGNDINDIFFAAVEMTRMPMIVSD 60 Query: 61 PNRPDNPIVFVNNAFINMTGYSSAEVVGKNCRFLQGPETDRSVVAEVRRAVAERREIATE 120 PNRPDNPIVFVNNAFINMTGYSSAEVVGKNCRFLQGPETDRSVVAEVRRAVAERREIATE Sbjct: 61 PNRPDNPIVFVNNAFINMTGYSSAEVVGKNCRFLQGPETDRSVVAEVRRAVAERREIATE 120 Query: 121 LLNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAEQALGQARKMEALGQLT 180 LLNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAEQALGQARKMEALGQLT Sbjct: 121 LLNYRKNGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAEQALGQARKMEALGQLT 180 Query: 181 GGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAFA 240 GGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAFA Sbjct: 181 GGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAFA 240 Query: 241 RKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPTQMEVALLNVLI 300 RKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPTQMEVALLNVLI Sbjct: 241 RKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWNCRLDPTQMEVALLNVLI 300 Query: 301 NARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDP 360 NARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDP Sbjct: 301 NARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDP 360 Query: 361 FFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQVGGGFEYDN 420 FFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQVGGGFEYDN Sbjct: 361 FFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPATQDQVGGGFEYDN 420 Query: 421 RTQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTD 480 R+QSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTD Sbjct: 421 RSQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTD 480 Query: 481 LIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDELARK 540 LIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDELARK Sbjct: 481 LIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDELARK 540 Query: 541 IRIVLDGATGVS 552 IRIVLDGATGVS Sbjct: 541 IRIVLDGATGVS 552 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 327 bits (838), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 198/504 (39%), Positives = 285/504 (56%), Gaps = 37/504 (7%) Query: 71 VNNAFINMTGYSSAEVVGKNCRFLQGPETDRSVVAEVRRAVAERREIATELLNYRKNGST 130 VN A M G S E++G + P+ ++ R + + R E R +GST Sbjct: 596 VNQALCRMLGRSEEEMLGMTLADVTHPDDVPMNMSLFNRCIEDGRSFTLEKRYLRPDGST 655 Query: 131 FWNALFISPVYDQQGNLKYFFSSQLDISRRRDAEQALG---------------------- 168 FW + IS + D +G + + DI+ RR AE+AL Sbjct: 656 FWVSSDISRIVDGEGRPQAMIAVSTDITERRRAEEALRELNDTLEQRVVEEILERSRAEA 715 Query: 169 ---QARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIRKA 225 Q +KMEA+GQLTGG+AHDFNN+LQ+I G L ++ R D+ RS +R A Sbjct: 716 ALRQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQSDPRLDE-------RSRQRVRIA 768 Query: 226 SG---KAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLW 282 G + A L+ QLLAFAR+Q L+ R+INL + + DL+ R +G +I + T +A LW Sbjct: 769 IGAVDRGAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGENIDISTRVAHDLW 828 Query: 283 NCRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAV 342 N LDP Q+E ++N INARDAM GR+ ++ N L DP Q L G YV + + Sbjct: 829 NTMLDPNQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQI 888 Query: 343 TDDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVT 402 +D+G G++P++ R +PFFTTK EG+GTGLGLSM YGFVKQS G + I S GTT+ Sbjct: 889 SDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIR 948 Query: 403 IYFPATQDQVGGGFEYDNRTQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPA 462 IYFP D+ E + T GG E ILVV+D + L SLGYRV ++ Sbjct: 949 IYFPRCLDEEVRIDEVRDLT-VHGGDETILVVEDDPNLQLTVIDTLSSLGYRVLKADNGE 1007 Query: 463 EALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHG-A 521 AL++++SG +DL+FTD++MPGS+ LA++A+ ++P I++L T+GY +++ G Sbjct: 1008 RALEILQSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILFTSGYPRDTIVHEGRL 1067 Query: 522 DGEFPVLDKPYRHDELARKIRIVL 545 D +L KPYR ++LA++IR +L Sbjct: 1068 DAGVELLSKPYRINQLAQRIRQML 1091 Score = 59.3 bits (142), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 41/115 (35%), Positives = 62/115 (53%), Gaps = 4/115 (3%) Query: 423 QSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTDLI 482 Q G +LVV+D ++ +L +LE+LG++ V S EALQ + + D+LFTD+ Sbjct: 1158 QEDAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEE-QGFDVLFTDVN 1216 Query: 483 MPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDEL 537 +PG M+GV LA+ A P + V+ +GY + G L KPY D+L Sbjct: 1217 LPG-MSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAH--ALPKPYDIDQL 1268 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 284 bits (727), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 169/388 (43%), Positives = 237/388 (61%), Gaps = 13/388 (3%) Query: 162 DAEQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADS 221 +AE+AL QA+KMEA+GQLTGG+AHDFNN+L I+G L+++ + L + + + R + Sbjct: 296 NAEEALRQAQKMEAVGQLTGGLAHDFNNILAGISGSLELMKVRLAQGR--IGDIERHLNG 353 Query: 222 IRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGL 281 + A +AA LTQ+LLAF+R+Q L+ + +LN + G +L+ R++G I V+T A GL Sbjct: 354 AQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIAAGGL 413 Query: 282 WNCRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIA 341 W+ +D QME ALLN+ INARDAM GR+ I TAN L D A+Q G+ G YVS++ Sbjct: 414 WHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLDDIG-ALQRGVPPGQYVSLS 472 Query: 342 VTDDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTV 401 V+D G G++P+++ R DPFFTTK G+GTGLGLSMVYGF QSGG+VRI S G V Sbjct: 473 VSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMV 532 Query: 402 TIYFPATQDQVGGG------FEYDNRTQSQGGQEHILVVDDRLEVAELAQTMLESLGYRV 455 IY P VG G ++ + G + ILVVDD V + +L LGY V Sbjct: 533 CIYLP---RHVGEGGVDDLALPEESHVPAAPGSKTILVVDDEPLVRMVTVEVLRDLGYSV 589 Query: 456 SMVNSPAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASES 515 AL+++ + + +DLL TD+ +P MNG LA + P + VL TGYA + Sbjct: 590 LEAEDGPSALRVVEACQEIDLLLTDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGYAENA 649 Query: 516 LERHGA-DGEFPVLDKPYRHDELARKIR 542 + HG + VL KP+ D LAR+++ Sbjct: 650 VLNHGHLERGMQVLTKPFAADVLARRVK 677 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 280 bits (716), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 165/387 (42%), Positives = 234/387 (60%), Gaps = 7/387 (1%) Query: 164 EQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLDMINLALRSDKPDLARVARSADSIR 223 E+AL ++K+EA+GQLTGG+AHDFNNLL I+G LD++ + L + + + R + Sbjct: 456 EEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRGE--ELLRYVELAA 513 Query: 224 KASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVSRTLGGDITVQTELARGLWN 283 +++ +AA LTQ+LLAFAR+Q L + +N+L G +L+ RT+G I ++ A GLW Sbjct: 514 RSTQRAASLTQRLLAFARRQTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWP 573 Query: 284 CRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANEDLADPDRAVQLGLKEGLYVSIAVT 343 +D Q+E ALLN+ INARDAM G GR+ I TAN+ L D A + L G YV I+VT Sbjct: 574 TFVDGPQLESALLNLCINARDAMPGGGRLTIETANKWL-DDRWAHERDLPPGQYVCISVT 632 Query: 344 DDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTI 403 D G G+ PD+L R DPFFTTK G GTGLGLSMVYGF +QSGG VR+ S GTTV+I Sbjct: 633 DSGVGMPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVSI 692 Query: 404 YFPATQ---DQVGGGFEYDNRTQSQGGQEHILVVDDRLEVAELAQTMLESLGYRVSMVNS 460 Y P Q D+ D G E +LVVDD + L +L GY V Sbjct: 693 YLPRHQHDGDEQDEADALDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAED 752 Query: 461 PAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLLTTGYASESLERHG 520 AL++++S + +DLL TD+ +PG +NG +A + P + VL TGYA + +G Sbjct: 753 GPGALRILQSKQRIDLLITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNG 812 Query: 521 A-DGEFPVLDKPYRHDELARKIRIVLD 546 + ++ KP++ + LAR+++ +L+ Sbjct: 813 HLERGMQLMTKPFKMEALARRVQSILE 839 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 110 bits (275), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 93/352 (26%), Positives = 159/352 (45%), Gaps = 23/352 (6%) Query: 68 IVFVNNAFINMTGYSSAEVVGKNCRF-LQGPETDRSVVAEVRRAVAERREIATELLNYRK 126 I +VN AF M G+++ E+VGK PE +A R E + R+ Sbjct: 329 ITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGRATPQFETIFQRR 388 Query: 127 NGSTFWNALFISPVYDQQGNLKYFFSSQLDISRRRDAE-------QALGQARKMEALGQL 179 +G F +F SP+ DQ G + S LDIS R+ AE + L + ++ ++G++ Sbjct: 389 DGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARLASMGEI 448 Query: 180 TGGIAHDFNNLLQVITGYLD-MINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLA 238 +AH+ N L I+ Y +NL ++ + D + + + I + +A + + + Sbjct: 449 ASTLAHELNQPLAAISSYTTGALNL-MQQGQADPKALQPALEKIHAQAQRAGHVIRSVHQ 507 Query: 239 FARKQRLEGRTINLNSLTEGFTDLVS-RTLGGDITVQTELARGLWNCRLDPTQMEVALLN 297 F +K+ + + + L + L+ + + +Q ++ R L D +E +LN Sbjct: 508 FVKKREPARQRVQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLGDQILLEQVILN 567 Query: 298 VLINARDAMNGRGRVR--IRTANEDLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILP 355 + N AM R +R A E + D A V+++VTD G GI D+ Sbjct: 568 LTRNGIQAMAAVPSARRILRVAAEVTRNEDGAES--------VTVSVTDQGHGIPDDVAA 619 Query: 356 RVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRITSGAEGGTTVTIYFPA 407 R+ PFF+TK E G G+GL++ ++ GG++ T GGT PA Sbjct: 620 RLFSPFFSTKAE--GMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPA 669 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,618,619 Number of extensions: 68538 Number of successful extensions: 435 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 413 Number of HSP's successfully gapped: 22 Length of query: 552 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 462 Effective length of database: 1,193,720 Effective search space: 551498640 Effective search space used: 551498640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)