BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0145.p (628 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 1181 0.0 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 158 1e-39 gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC t... 152 9e-38 gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 134 3e-32 gi|300311564|ref|YP_003775656.1| branched-chain amino acid ABC t... 118 2e-27 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 1181 bits (3055), Expect = 0.0, Method: Compositional matrix adjust. Identities = 624/628 (99%), Positives = 625/628 (99%) Query: 1 MTLASLAVQLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLG 60 MTLASLAVQLLNGLADASAMF+VAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLG Sbjct: 1 MTLASLAVQLLNGLADASAMFMVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLG 60 Query: 61 ATPWGYWAALLLAALLVAVLGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWG 120 ATPWGYWAALLLAAL VA LGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWG Sbjct: 61 ATPWGYWAALLLAALPVAALGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWG 120 Query: 121 PEDLFGPRAPGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQD 180 PEDLFGPRAPGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQD Sbjct: 121 PEDLFGPRAPGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQD 180 Query: 181 REMLGALGVNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGM 240 REMLGALGVNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGM Sbjct: 181 REMLGALGVNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGM 240 Query: 241 GSIGGAFAAAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLG 300 GSIGGAFAAAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLG Sbjct: 241 GSIGGAFAAAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLG 300 Query: 301 KPLALVRAASHHEAPLRAASQRERWAAMVLLAALLALPLLAGRFPYLPVLMVEILVAVLF 360 KPLALVRAASHHEAPLRAASQRERWAAMVLLAALLALPLLAGRFPYLPVLMVEILVAVLF Sbjct: 301 KPLALVRAASHHEAPLRAASQRERWAAMVLLAALLALPLLAGRFPYLPVLMVEILVAVLF 360 Query: 361 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAMLAALLFGW 420 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAMLAALLFGW Sbjct: 361 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAMLAALLFGW 420 Query: 421 FCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRTTAALASPLAYYYLALLC 480 FCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRT AALASPLAYYYLALLC Sbjct: 421 FCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRTAAALASPLAYYYLALLC 480 Query: 481 SALGVYLLRRVIHAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAGVLFA 540 SALGVYLLRRVIHAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAGVLFA Sbjct: 481 SALGVYLLRRVIHAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAGVLFA 540 Query: 541 FSKGSISPEVAGVNRSVDGLVMVLLGGVQSLAGPLLGASVFTWLQDLVARETDYWRALLG 600 FSKGSISPEVAGVNRSVDGLVMVLLGGVQSLAGPLLGASVFTWLQDLVARETDYWRALLG Sbjct: 541 FSKGSISPEVAGVNRSVDGLVMVLLGGVQSLAGPLLGASVFTWLQDLVARETDYWRALLG 600 Query: 601 GVILLLVLLLPGGLAGAWRRAGRPGEGA 628 GVILLLVLLLPGGLAGAWRRAGRPGEGA Sbjct: 601 GVILLLVLLLPGGLAGAWRRAGRPGEGA 628 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 115/297 (38%), Positives = 162/297 (54%), Gaps = 12/297 (4%) Query: 9 QLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLGATPWGYWA 68 Q+LN L +FL+A+GL+LIFGV VN AHGSF+M+G Y+A + G+ +W Sbjct: 8 QVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFMIGAYVAAVVAGASGS----FWL 63 Query: 69 ALLLAALLVAVLGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWGPEDLFGPR 128 LL+A A LG L+E + RR+Y+ + Q+LATFA +L+ D WGP+ L Sbjct: 64 GLLVALPATAALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRMIWGPQPLPLNM 123 Query: 129 APGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQDREMLGALG 188 AG V + G Y LL+MA+G +V L+ L+NRTR G +RA + EM+ A+G Sbjct: 124 PAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAGANNLEMVTAMG 183 Query: 189 VNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGMGSIGGAFA 248 +N LF VF+ G LA L G + GP + + D + AFVVVV+GG+GS+ GA Sbjct: 184 INIRLLFRLVFSFGLLLAALAGIMLGPLVAVQAGMGDDVLILAFVVVVIGGIGSVPGAAI 243 Query: 249 AAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAE-------FLVMAVILVVRPWGL 298 A+++ V L +L F + LS V +LVM +L RP GL Sbjct: 244 GALIVGVVDTLGRSL-LPQLFALFLSADAAASVGPALGSMMIYLVMIAVLFWRPSGL 299 >gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 152 bits (384), Expect = 9e-38, Method: Compositional matrix adjust. Identities = 114/296 (38%), Positives = 176/296 (59%), Gaps = 19/296 (6%) Query: 9 QLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLGATPWGYWA 68 Q+ NG+ FL+A GLS++FG+ R VNF+HG+F++LG YL + + D LG G+W Sbjct: 7 QIANGVGIGMLYFLLAVGLSIVFGLLRFVNFSHGAFFLLGAYLCFQVTD-LGL---GFWW 62 Query: 69 ALLLAALLVAVLGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWGPEDLFGPR 128 +LL+A L+V + +VE +LR +Y P F +L T + LVI++A + WGP L Sbjct: 63 SLLIAPLVVGLFAWIVERFVLRHVYALPHHFHILFTVGMALVIQEAVISTWGP--LGNNV 120 Query: 129 APG--LAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQDREMLGA 186 AP L G V G P+Y L ++ + ++ LLW +L TR G+++RA ++ EM+ Sbjct: 121 APPDLLQGVVMWGGFVYPKYRLFVIGLTAVMALLLWWVLEGTRLGSIVRAGSESTEMVSL 180 Query: 187 LGVNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGMGSIGGA 246 LG+N +F+ VFALG AGL GAL P A+ + ++ +G AFVVVV+GG+GS GA Sbjct: 181 LGLNINRIFSLVFALGAATAGLAGALAAPIRGADPFMGVEALGIAFVVVVIGGLGSFWGA 240 Query: 247 FAAAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLGKP 302 +L+ V+++ + TV G L + ++ MA ++++RP GLLG+P Sbjct: 241 LIGGILVGIVQSV---MSTVWSEGAHLMI--------YVAMAAVMLLRPNGLLGRP 285 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 134 bits (336), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 93/286 (32%), Positives = 153/286 (53%), Gaps = 14/286 (4%) Query: 16 DASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLGATPWGYWAALLLAAL 75 + S +++ GL++IFG+ ++NFAHG+ YMLG +LA+ + LG YW L++A + Sbjct: 21 NGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLN---YWVMLIVAPI 77 Query: 76 LVAVLGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWGPEDLFGPRAPGLAGA 135 LV G ++E T+LR +Y+ L+ LL TF + L+I+ +G L GA Sbjct: 78 LVGAFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVPDLLQGA 137 Query: 136 VQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQDREMLGALGVNQAWLF 195 + LP Y ++ +V W ++ +TR GA +RA T++ +++ A G+N + Sbjct: 138 TNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGINVPLMV 197 Query: 196 TGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGMGSIGGAFAAAVLIAE 255 T + G LA G L P + + + + I T F VVV+GGMGSI G+ +++ Sbjct: 198 TLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIITGLVLGL 257 Query: 256 VKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLGK 301 ++ L F ELS + + F++MA++L+VRP GL GK Sbjct: 258 IEGLT------RVFYPELSATVV-----FIIMAIVLMVRPAGLFGK 292 >gi|300311564|ref|YP_003775656.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 118 bits (295), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 102/303 (33%), Positives = 148/303 (48%), Gaps = 22/303 (7%) Query: 327 AMVLLAALLALPLLAGRFPYLPVLMVEILVAVLFAASLHFLMGPGGMVSFGHAAYFGLGA 386 A+VLLAA L P A L+ ++ VL S + L+G GGM+SFGHA Y GLGA Sbjct: 14 ALVLLAAPLVFPQSAAL-----SLLSQMGTMVLLCLSYNMLLGEGGMLSFGHAVYSGLGA 68 Query: 387 YAAALVTLR-----LQWPMPLAMLAGAGTAMLAALLFGWFCVRLSGVYLAMLTLAFAQIV 441 + A + R L +PM L LAG T L +L G+ R +G AM+TL ++V Sbjct: 69 FGAIHLMNRMAEGALAFPMTLVPLAGGLTGALCGVLLGYLTTRRAGTTFAMITLGLVELV 128 Query: 442 WAAIYQWDDLTGGSNGLVGLRTTAALASPLAY------YYLA---LLCSALGVYLLRRVI 492 A + + ++ GG G+ G R ++Y YYL LL A+ ++ LR Sbjct: 129 SACVLMFPEVFGGEGGISGNRVIGEPVLGISYGPQIQVYYLIAGWLLVCAIAIWALRAT- 187 Query: 493 HAPFGLALRAARDASGRAQALGMDVRALQWGGFALAGLFCGLAGVLFAFSKGSISPEVAG 552 P G L A RD RA +G D + +++ L+ F G++G L A + +S E G Sbjct: 188 --PLGRILNAVRDNPERAAFIGYDPQRVRYLALILSAFFAGVSGGLSAINFEIVSAEYVG 245 Query: 553 VNRSVDGLVMVLLGGVQSLAGPLLGASVFTWLQDLVARETDYWRALLGGVILLLVLLLPG 612 RS L+ +GG GP++G V ++ T W+ LG +L+V+ PG Sbjct: 246 ALRSGAILLFTFIGGSAYFYGPIVGGVVGVLFSSWLSAYTQAWQFYLGAFFILIVMTAPG 305 Query: 613 GLA 615 GLA Sbjct: 306 GLA 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,370,541 Number of extensions: 45106 Number of successful extensions: 418 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 367 Number of HSP's successfully gapped: 38 Length of query: 628 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 537 Effective length of database: 1,188,985 Effective search space: 638484945 Effective search space used: 638484945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)