BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0144.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 812 0.0 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 338 6e-94 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 98 1e-21 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 92 6e-20 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 82 6e-17 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 812 bits (2097), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/404 (98%), Positives = 402/404 (99%) Query: 1 VNQFNFQRGLRWALAAALLAMGSVAARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDE 60 +NQFNFQRGLRWALAAALL MGSVAARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDE Sbjct: 1 MNQFNFQRGLRWALAAALLTMGSVAARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDE 60 Query: 61 INASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ 120 INASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ Sbjct: 61 INASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ 120 Query: 121 HKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYG 180 H+VFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYG Sbjct: 121 HRVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYG 180 Query: 181 QSAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKF 240 QSAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKF Sbjct: 181 QSAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKF 240 Query: 241 VREGNIRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAINTPEHKKFVEAYR 300 VREGN RGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAI+TPEHKKFVEAYR Sbjct: 241 VREGNTRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAIHTPEHKKFVEAYR 300 Query: 301 KKFNDYPRNGSLVGYSALMSIAAGLRKAGSTDADRLAAAFSGLKVETPVATIVYRPQDHQ 360 KKFNDYPRNGSLVGYSALMSIAAGLRKAGSTDADRLAAAFSGLKVETPVA+IVYRPQDHQ Sbjct: 301 KKFNDYPRNGSLVGYSALMSIAAGLRKAGSTDADRLAAAFSGLKVETPVASIVYRPQDHQ 360 Query: 361 STLGAFVGRTGLKEGRGVMTSFTYVDGAALQLPDAEVKKLRAPD 404 STLGAFVGRTGLKEGRGVMTSFTY+DGAALQLPDAEVKKLRAPD Sbjct: 361 STLGAFVGRTGLKEGRGVMTSFTYIDGAALQLPDAEVKKLRAPD 404 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 338 bits (866), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 167/376 (44%), Positives = 248/376 (65%) Query: 26 ARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDEINASGGVLGKKLEVIARDDNGNPGD 85 + A + IRIGEINS + YR G +AL++INA+GGV G+ L+++ R+D+G+ Sbjct: 32 SHAAEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDADQ 91 Query: 86 SVRVAEELMARERVALLFGGFLSNTGLALTDYAKQHKVFFLAAEPLTDKIVWQNGNKYTY 145 + R A L++++ V +L G LS+ LA++ A + + + + L+D I GN+YT+ Sbjct: 92 AARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRYTF 151 Query: 146 RLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVAEQA 205 R+RPST M A + ++A +L +RWA+V PNYEYGQSAVASFK L+KQA+PDVEFV++Q Sbjct: 152 RVRPSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSDQW 211 Query: 206 PPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNIRGLFEQVDVVSLLTGEPEY 265 P LGKI A V +A+ ++PD IFNA F DL KFV+ GN + LFE V VVS+ + Sbjct: 212 PALGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGAPS 271 Query: 266 LDPLKGEAPQNWIVTGYPWYAINTPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSIAAGL 325 LDP + EA ++W+ GYPW ++ PEH++F+EAY K+F++ P+ ++ GY+ +M+IAAGL Sbjct: 272 LDPSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAAGL 331 Query: 326 RKAGSTDADRLAAAFSGLKVETPVATIVYRPQDHQSTLGAFVGRTGLKEGRGVMTSFTYV 385 +K+ D + L A GL+ +P+ + +R D Q++LGAFVG K+GR M + Y+ Sbjct: 332 KKSEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWRYL 391 Query: 386 DGAALQLPDAEVKKLR 401 DGAA P A V+ R Sbjct: 392 DGAAYLPPPAYVRSRR 407 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 108/388 (27%), Positives = 183/388 (47%), Gaps = 37/388 (9%) Query: 14 LAAALLAMGSVAARAQDTIRIGEINSYKAQPAFLE-PYRRGWEMALDEINASGGVLGKKL 72 +AAAL+++ ++ ++A D I+IG Y A + R G ++A+ EINA+GG+LG+++ Sbjct: 9 VAAALMSL-ALTSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGRQI 67 Query: 73 EVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDY-----AKQHKVFFLA 127 ++I RDD V++A+EL+ +E+V GF+ NTG+AL AK + +A Sbjct: 68 QLIERDDEAKNERGVQIAQELINKEKVVATV-GFI-NTGVALASQRFYQEAKIPVINNVA 125 Query: 128 AEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAV-KLKKKRWAIVYPNYEYGQSAVAS 186 + K + + Y +R + + + EAV K K + AI+ + YGQ Sbjct: 126 TGTVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGRED 185 Query: 187 FKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFVREGNI 246 + ++ A V VA + + +D A A+ +L + + Sbjct: 186 LEKVL--ADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQIAN--GM 241 Query: 247 RGLFEQVDVVS---LLTGEPEYLDPL-----KGEAPQNWIVTGYPWYAINTPEHKKFVEA 298 L V ++ L G ++D PQ +I G NTP+ K F+EA Sbjct: 242 EKLNWHVPIIGSWPLSMG--NFIDNSGKNGNGARMPQTFIQDG------NTPKRKAFIEA 293 Query: 299 YRK--KFNDYPRNGSLV-GYSALMSIAAGLRKAGSTDADRLAAAFSGL--KVETPVATI- 352 Y+K K + P S GY +++ +AA +++AG+TD D++ AA L KVE V T Sbjct: 294 YQKAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGDKVRAALENLNTKVEGVVTTYD 353 Query: 353 -VYRPQDHQSTLGAFVGRTGLKEGRGVM 379 + DH++ A V +K GR ++ Sbjct: 354 KPFTHDDHEAIKPAMVVMGEVKNGRVIL 381 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 92.4 bits (228), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 82/313 (26%), Positives = 142/313 (45%), Gaps = 15/313 (4%) Query: 51 RRGWEMALDEINASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNT 110 + G+ +A +EINA GGV G KL ++ D+ G +++ V ++L+ +++V +FG LSN+ Sbjct: 48 KNGFTLASEEINAKGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNS 107 Query: 111 GLALTDYAKQHKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVK-LKKKR 169 A A KV D I +T+R + ++ VK AVK K+ Sbjct: 108 AFAADPIANAAKVVVFGTSNTADGITAM--GPFTFRNSVMEADVLPVTVKAAVKHFGIKK 165 Query: 170 WAIVYPNYE-YGQSAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAI 228 A++Y N + + +S FK ++ + + +A G +D A + PDAI Sbjct: 166 VAVIYGNDDAFTKSGYDVFKATLEHQK--IPVTTTEAYAKGDVDFKAQLTKIKAGNPDAI 223 Query: 229 FNALFAADLGKFVREGNIRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAIN 288 + A + + + G+ +Q + P+ + K +A N ++ G PW A N Sbjct: 224 VCSCLAEEAANIILQTRALGM-KQPFIGGNGLNSPKLFEIAK-DAGDNTLM-GSPWSAEN 280 Query: 289 -TPEHKKFVEAYRKKFNDYPRNGSLVGYSALMSIAAGLRKAG-----STDADRLAAAFSG 342 TP +K F+ AY+ KF P + Y A+ +A L+ + D D L AA Sbjct: 281 QTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALPA 340 Query: 343 LKVETPVATIVYR 355 +K++ +R Sbjct: 341 VKIDGATGKFAFR 353 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 83/313 (26%), Positives = 134/313 (42%), Gaps = 15/313 (4%) Query: 53 GWEMALDEINASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLF-GGFLSNTG 111 G ++AL + G VLGK + D G P +VR +E + E+ F GG LS Sbjct: 53 GMKLALQKY---GKVLGKSTSYVNLDTEGKPATAVRKMQESI--EKGTRFFAGGILSGEA 107 Query: 112 LALTDYAKQHKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKL--KKKR 169 LA+ A + F+ D+I + N+ T+R T + V+ ++ KR Sbjct: 108 LAMGKEADKAGAVFITTAG-ADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKR 166 Query: 170 WAIVYPNYEYGQSAVASFKTLMKQAQPDVEFVAEQAPPLGKID-AGAVTQALADAKPDAI 228 W + P Y +G+ +++ K + K+ +E V L + + +G +T A+A AKPD + Sbjct: 167 WYTITPQYVFGEGLLSAAKNIFKE--KGIEHVGNSYHSLTEKEFSGYLTNAIA-AKPDVL 223 Query: 229 FNALFAADLGKFVREGNIRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAIN 288 F + +R+ G+ ++ E + L + W+AI+ Sbjct: 224 LLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAID 283 Query: 289 TPEHKKFVEAYRKKFNDYPRNGSLVG-YSALMSIAAGLRKAGSTDADRLAAAFSGLKVET 347 P +K+FV+ P N SL G Y I + KA STD + AA G+K E Sbjct: 284 APFNKEFVKLVNDNLKIDP-NYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEG 342 Query: 348 PVATIVYRPQDHQ 360 + R DHQ Sbjct: 343 LTGSEEIRKGDHQ 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,256,963 Number of extensions: 54192 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 19 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)