BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0142.p (460 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311774|ref|YP_003775866.1| two component sensor histidine ... 924 0.0 gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine ... 118 1e-27 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 65 1e-11 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 60 5e-10 gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 57 4e-09 >gi|300311774|ref|YP_003775866.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 460 Score = 924 bits (2389), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/460 (99%), Positives = 459/460 (99%) Query: 1 MTAADAVAKRRTLRERWQRLRQGEQNPAGHQNMLLLIQLRWLAVLGQVSTIAFVVLVFDL 60 MTAADAVAKRRTLRERWQRLRQGEQNPAGHQNMLLLIQLRWLAVLGQVSTIAFVVLVFDL Sbjct: 1 MTAADAVAKRRTLRERWQRLRQGEQNPAGHQNMLLLIQLRWLAVLGQVSTIAFVVLVFDL 60 Query: 61 HLPLPHMLEVLSCLVAFNIASHLRWHERRIATNAELFLALLVDVAVLTFQLYLSGGITNP 120 HLPLPHMLEVLSCLVAFNIASHLRWHERRIATNAELFLALLVDVAVLTFQLYLSGGITNP Sbjct: 61 HLPLPHMLEVLSCLVAFNIASHLRWHERRIATNAELFLALLVDVAVLTFQLYLSGGITNP 120 Query: 121 FAFLYLMQIILSAVLLKPWSSWAIVGVTMLCLAGLALFSEPLALPPDHNAGLLSLYVEGT 180 FAFLYLMQIILSAVLLKPWSSWAIVGVTMLCLAGLALFSEPLALPPDHNAGLLSLYVEGT Sbjct: 121 FAFLYLMQIILSAVLLKPWSSWAIVGVTMLCLAGLALFSEPLALPPDHNAGLLSLYVEGT 180 Query: 181 LICFLLNAGLMVFFITRISSNLRAGDALLADLRQRAAEEDHIVRMGLLASGAAHELGTPL 240 LICFLLNAGLMVFFITRISSNLRAGDALLADLRQRAAEEDHIVRMGLLASGAAHELGTPL Sbjct: 181 LICFLLNAGLMVFFITRISSNLRAGDALLADLRQRAAEEDHIVRMGLLASGAAHELGTPL 240 Query: 241 ATVSVILGDWKRMPEISQRQDMLDDIAEMEAQLQRCKSIVSGVLLSAGQARGESSIKTTL 300 ATVSVILGDWKRMPEISQRQDMLDDIAEMEAQLQRCKSIVSGVLLSAGQARGESSIKTTL Sbjct: 241 ATVSVILGDWKRMPEISQRQDMLDDIAEMEAQLQRCKSIVSGVLLSAGQARGESSIKTTL 300 Query: 301 NTFLDSVAEDWRRSRPVAVFDYQNLIGQDLPVAFDSAIKQMLGNVLDNAFEVSPHWLSLE 360 NTFLDSVAEDWR+SRPVAVFDYQNLIGQDLPVAFDSAIKQMLGNVLDNAFEVSPHWLSLE Sbjct: 301 NTFLDSVAEDWRKSRPVAVFDYQNLIGQDLPVAFDSAIKQMLGNVLDNAFEVSPHWLSLE 360 Query: 361 VRREGGLLLMEVSDQGPGFPEWMINQIGKPYQSTKGKPGRGLGLFFVVNVARKLGGRVWA 420 VRREGGLLLMEVSDQGPGFPEWMINQIGKPYQSTKGKPGRGLGLFFVVNVARKLGGRVWA Sbjct: 361 VRREGGLLLMEVSDQGPGFPEWMINQIGKPYQSTKGKPGRGLGLFFVVNVARKLGGRVWA 420 Query: 421 SNLPGAGARVTLELPLSAISLEADAEPVAGSATPAFPSGA 460 SNLPGAGARVTLELPLSAISLEADAEPV GSATPAFPSGA Sbjct: 421 SNLPGAGARVTLELPLSAISLEADAEPVVGSATPAFPSGA 460 >gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 118 bits (295), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 135/434 (31%), Positives = 212/434 (48%), Gaps = 33/434 (7%) Query: 27 PAGHQNMLLLIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCLVAFNIASHLRWH 86 P H + L LRW V G++ +A + LP+P +L ++ N A+ LR Sbjct: 15 PTQHVLLSRLFWLRWAMVAGELLVLAGAHGWLHIALPVPQLLALIGLQALLNGATWLRLR 74 Query: 87 ERRIATNAELFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIVG 146 R A ELFL LL+D+AVLT LY SGG TNPF YL + A L + + Sbjct: 75 WTRPAEQRELFLQLLLDLAVLTALLYFSGGSTNPFVSFYLPALAAGAASLAWPYALLLAL 134 Query: 147 VTMLCLAGLALFSEPLALPPDHNAG-LLSLYVEGTLICFLLNAGLMVFFITRISSNLRAG 205 + +GL EPL + H+ G ++ ++ G I F ++A L+ +F++RI++ +R+ Sbjct: 135 CALGAYSGLVYVYEPLHI---HDQGQAMAYHLAGMWINFSISALLITWFVSRIAAAVRSR 191 Query: 206 DALLADLRQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKR----MPEISQRQD 261 + LA R+R + I+ +G A+GAAH L TPL+TV+VI G+ +R P ++ + Sbjct: 192 ETELARARERQLQSQRILALGTQAAGAAHALNTPLSTVAVIAGELRRDMATQPALAAYDE 251 Query: 262 MLDDIAEMEAQLQRCKSIVSGVLLSA-GQARGESSIKTTLNTFLDSVAEDWRRSRPVAVF 320 L + E Q+ CK+ + + A Q R E S T +L ++ E WR P + Sbjct: 252 ELRVV---EEQIAVCKAALESMRQDADAQTRNEDSAPIT--PWLTTLLEGWRLRHPAVLL 306 Query: 321 DYQNLIGQDLPVAFD-SAIKQMLGNVLDNAFEV-----SPHWLSLEVRREG--------G 366 + + Q VA + Q+L +LDNA S +S+ +R Sbjct: 307 SAE--VHQPGWVAARLEDLSQILLTLLDNAAHAVRERGSEGRVSIRMRAADEPAGRPAQK 364 Query: 367 LLLMEVSDQGPGFPEWMINQIGK-PYQSTKGKPGRGLGLFFVVNVARKLGGRVWASNLPG 425 ++E++D G G ++ ++G+ P S+ G G GL L F A LGG++ + PG Sbjct: 365 AAIIEIADNGSGIAPELLQRLGRAPVASSSGGSGIGLMLAFA--SAAHLGGKLRLYSTPG 422 Query: 426 AGARVTLELPLSAI 439 G L LPLSA+ Sbjct: 423 QGTCAELRLPLSAM 436 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 73/280 (26%), Positives = 125/280 (44%), Gaps = 49/280 (17%) Query: 218 EEDHIVRMGLLASGAAHELGTPLATVSV---ILGDW------------------------ 250 + + + +G LA+G AHE+ P+ VS LG++ Sbjct: 180 QSEKLASIGQLAAGVAHEINNPIGFVSANLNTLGNYVATLLEGIQLHQCLQGGALPAAMA 239 Query: 251 -KRMPEISQRQDML---DDIAEMEAQ----LQRCKSIVSGVLLSAGQARGESSIK-TTLN 301 +++ R D+ +D+ E+ A+ LQR ++IV L GES+ + T L+ Sbjct: 240 QEQLRSFEARADLAFLSEDVTELLAESNEGLQRVRNIVQS--LKDFSRIGESNWQMTDLH 297 Query: 302 TFLDS---VAEDWRRSRPVAVFDYQNLIGQDLPVAFDSAIKQMLGNVLDNAFEVSPH--W 356 LDS +A + + + V Y L L S + Q+ N+L NA + Sbjct: 298 RGLDSTLNIANNELKYKVSIVKQYGEL---PLVKCLASELNQVFMNLLVNAAQAISERGT 354 Query: 357 LSLEVRREGGLLLMEVSDQGPGFPEWMINQIGKPYQSTKG-KPGRGLGLFFVVNVARKLG 415 +++ R G + + +SD G G +N+I +P+ +TK G GLGL + +K G Sbjct: 355 VTICTARAGDKVSVSISDTGHGIAPENLNRIFEPFFTTKPVGSGTGLGLSLSYGIVKKHG 414 Query: 416 GRVWASNLPGAGARVTLELPLSAISL--EADAEPVAGSAT 453 G + S+ PG G T+ELP+ S+ ++ A +G A+ Sbjct: 415 GDIRVSSAPGCGTTFTIELPIDPASVLAQSSATETSGEAS 454 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 66/239 (27%), Positives = 109/239 (45%), Gaps = 39/239 (16%) Query: 198 ISSNLRAGDALLADLRQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEIS 257 IS +R+ + +LA L Q A + + + AAH++ TPLA + + E++ Sbjct: 128 ISPLVRSLNDMLARLSQTIAIQKRFI------ADAAHQMKTPLAGM-------RMQSELA 174 Query: 258 QRQDMLDDIAEMEAQLQRCKSIVSGV---LLSAGQARGESS-----IKTTLNTFLDSVAE 309 RQD D+ QL + + + LLS +A + S I LN SV + Sbjct: 175 LRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVVQ 234 Query: 310 DWRRSRPVAVFDYQNLIGQ--DLPVAFDSA---IKQMLGNVLDNAFEVSPH----WLSLE 360 DW P++ +L + D P+ ++++L N+LDNA +PH +++ Sbjct: 235 DWV---PMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVR 291 Query: 361 VRRE--GGLLLMEVSDQGPGFPEWMINQIGKPYQSTKGKP----GRGLGLFFVVNVARK 413 R + GGL ++EV D GPG P + + + G P G GLGL V +A++ Sbjct: 292 ARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQ 350 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 56.6 bits (135), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 76/290 (26%), Positives = 122/290 (42%), Gaps = 39/290 (13%) Query: 195 ITRISSNLRAGD---ALLADLRQRAAEEDHIVRMGL--LASGAAHELGTPLATVS----V 245 I IS RA + AL ADL+ R A H V L + +H+L PL +S + Sbjct: 356 IYDISDRKRAEEEIEALNADLK-RQALHLHSVNKELESFSYSVSHDLRAPLRAISGYAMI 414 Query: 246 ILGDWKRMPEISQRQDMLDDIAEMEAQL-----QRCKSIVSGVLLSAGQARGESSIKT-T 299 + D+ D +D+ + Q+ ++ +++ +L A GE +++ + Sbjct: 415 LEEDYA---------DAIDEKGREQLQVIRRNVRKMDELINDLLKLAKSTTGELTLERFS 465 Query: 300 LNTFLDSVAEDWRRSRPVAVFDYQNLIGQDLPVAFDSAIKQMLGNVLDNAFEVSPH---- 355 ++ + V RR P +FD L G VA I Q+ N+L NA + S Sbjct: 466 MDDLVRQVIAGLRRENPNVLFDVPPLEG---AVANRGLITQVWENLLSNAVKFSSKSEQP 522 Query: 356 --WLSLEVRREGGLLLMEVSDQGPGFPEWMINQIGKPYQSTKGKP---GRGLGLFFVVNV 410 +S++V E + V D G GF +++ +Q + G G+GL V + Sbjct: 523 AVRVSMQVEPEE--YIYGVHDNGVGFDMRYAHKLFGTFQRLHRQEEYAGTGIGLALVQRI 580 Query: 411 ARKLGGRVWASNLPGAGARVTLELPLSAISLEADAEPVAGSATPAFPSGA 460 + GRVWA + PG GA LP +S AGS + F + A Sbjct: 581 VIRHTGRVWAQSKPGEGASFYFSLPRKGLSPSLVERREAGSGSSTFTNEA 630 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,362,226 Number of extensions: 55627 Number of successful extensions: 295 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 13 Length of query: 460 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 372 Effective length of database: 1,203,190 Effective search space: 447586680 Effective search space used: 447586680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)