BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0136.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 880 0.0 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 144 1e-35 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 137 2e-33 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 122 9e-29 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 116 5e-27 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 880 bits (2274), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/447 (100%), Positives = 447/447 (100%) Query: 1 MASTSIHNGQGSGELGPHSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVYALASVL 60 MASTSIHNGQGSGELGPHSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVYALASVL Sbjct: 1 MASTSIHNGQGSGELGPHSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVYALASVL 60 Query: 61 VFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS 120 VFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS Sbjct: 61 VFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFS 120 Query: 121 TAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALGQ 180 TAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALGQ Sbjct: 121 TAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALGQ 180 Query: 181 LGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK 240 LGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK Sbjct: 181 LGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAK 240 Query: 241 LLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREATDFL 300 LLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREATDFL Sbjct: 241 LLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREATDFL 300 Query: 301 VVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAGQNTFIL 360 VVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAGQNTFIL Sbjct: 301 VVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAGQNTFIL 360 Query: 361 IGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY 420 IGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY Sbjct: 361 IGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGMAY 420 Query: 421 VSFYLLSGAVGSLAALSLNRALEARDD 447 VSFYLLSGAVGSLAALSLNRALEARDD Sbjct: 421 VSFYLLSGAVGSLAALSLNRALEARDD 447 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 144 bits (364), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 91/321 (28%), Positives = 163/321 (50%), Gaps = 18/321 (5%) Query: 18 HSTHPSTSHDHGKIEPGEIAVGVVIGRASEYFDFFVYALASVLVFPAVFFPNEPKLEGTL 77 ST + H K + A IG A EY+DFF+YA A+ L+FP +FFP + Sbjct: 2 QSTQSNAHHQQSK----KAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAII 57 Query: 78 YAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFSTAGIALLPTFEHIGVWA 137 + + ++ARPIG + + +GR+ L +F++GFST G+ LLPT++ +G+WA Sbjct: 58 ASLATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWA 117 Query: 138 IILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAALGQLGAPLGFVVAASLFAYM 197 LL ++R+ QG A+ G G S++ ++P +RG++++ G G ++AA++F + Sbjct: 118 PALLVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPL 177 Query: 198 LSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEYAKLLDERELE-PAGVVELA 256 + F WGWR PF ++F + + R + P + K D+ EL+ + VV+ Sbjct: 178 AHYMPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVF-KAADKEELKRRSPVVDAF 236 Query: 257 TTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREA-------TDFLVVQIWGGLI 309 N++ + AL +++ V + Y+++ A +L + + G ++ Sbjct: 237 RDCSPNML-----RVVCMALMNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIV 291 Query: 310 MAVGIVISGLIADRFGRRNTL 330 + I G ++DR GRR + Sbjct: 292 AVLVIPYVGKLSDRIGRRPPI 312 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 109/415 (26%), Positives = 190/415 (45%), Gaps = 7/415 (1%) Query: 38 VGVVIGRASEYFDFFVYALASVLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIF 97 + ++G A E++DF VY S ++ +FFP + + L A F F RP+G + Sbjct: 12 IAAIVGNALEWYDFIVYGFFSAII-ARLFFPADNEYTSLLVALATFGIGFFMRPVGGVLL 70 Query: 98 MAIQKHYGREVKLTLALFMLGFSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWD 157 GR+ + + + ++ + A IA PT+ IG+ A I++ + R+ QG A GG + Sbjct: 71 GLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFATGGEYS 130 Query: 158 GLPSLLAMNAPQHKRGWYAALGQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYV 217 + L +AP HKRG Y + +G L A + A + +L+ WGWR PF + Sbjct: 131 SSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWRLPFVL 190 Query: 218 AFAINVVALFSRLRLVSAPEYAKLLDERELEPAGVVELATTQGRNIIIGALAALASYALF 277 I V L+ R + ++ + ++ P ++++ T R +++ + F Sbjct: 191 GLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAF 250 Query: 278 HLVTV-FPLSWIKLYSLREATDFLVVQIWGGLIMAVGIVISGLIADRFGRRNTL--GSMA 334 ++V V P K L F+V I L+ AV I +G ++DR GRR L S+A Sbjct: 251 YVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAV-IPFAGALSDRLGRRTVLLYSSLA 309 Query: 335 LLIAILSAFVPTLMNGGEAGQNTFILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTS 394 L+ + FV ++ ++G ++G A++ F R R TG A+ Sbjct: 310 FLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAY 369 Query: 395 DLAWLVGAGFAPLVALGLSAHFG--MAYVSFYLLSGAVGSLAALSLNRALEARDD 447 +LA ++ GFAP + L+ G +A + L + +G AA ++ A + Sbjct: 370 NLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRDQSPAAKN 424 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 122 bits (305), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 103/371 (27%), Positives = 174/371 (46%), Gaps = 8/371 (2%) Query: 38 VGVVIGRASEYFDFFVYALASVLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIF 97 V VIG E+FDF VY+ +V++ +FFP L L A F F RP+G + Sbjct: 24 VATVIGNGLEWFDFTVYSFFAVII-AKLFFPTGNDLTSLLLAVATFGVGFFMRPVGGILL 82 Query: 98 MAIQKHYGREVKLTLALFMLGFSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWD 157 GR+ L++ + ++ T I L PT+E IG++A +++ + R+ QG + GG Sbjct: 83 GIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMG 142 Query: 158 GLPSLLAMNAPQHKRGWYAALGQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYV 217 + L AP+ +R +Y++ Q + ++ A++ ++ +SL A WGWR PF + Sbjct: 143 SATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLL 202 Query: 218 AFAINVVALFSRLRLVSAPEYAKLLDERELEPAGVVELATTQGRNIIIG-ALAALASYAL 276 I V + R L P + L+E++ + + E+ R ++ L + Sbjct: 203 GIIIGPVGYYIRHHLEETPTF---LEEKDKTDSPLKEVIRNFPRETSASFSMVILWTVCT 259 Query: 277 FHLVTVFPLSWIKLYSLREATDFLVVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALL 336 + L+ P +K+ L + T F+ + GGL + V I G +ADR GRR L A L Sbjct: 260 YVLLFYMPTYSVKVLKLPQTTGFIAGMV-GGLCIMVLAPIVGRLADRIGRRPLLSGSAAL 318 Query: 337 IAILSAFVPTLMNGGEAGQNTFI--LIGFALLGLSYGQAAGAVTANFKARYRYTGAALTS 394 I +L+ + +N + I L+ L+ G A + F A+ TG ++ Sbjct: 319 ILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAY 378 Query: 395 DLAWLVGAGFA 405 +LA + GFA Sbjct: 379 NLAVTIFGGFA 389 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 116 bits (290), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 116/408 (28%), Positives = 186/408 (45%), Gaps = 10/408 (2%) Query: 42 IGRASEYFDFFVYALASVLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQ 101 IG A E+FD +Y A +V FFP L F F +F RP+G + A Sbjct: 26 IGNALEWFDILIYG-AFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYS 84 Query: 102 KHYGREVKLTLALFMLGFSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPS 161 GR+ L L++ ++ TA IA +P++ IG+ A ++L ++ QG + GG + + Sbjct: 85 DRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTA 144 Query: 162 LLAMNAPQHKRGWYAALGQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAI 221 L +AP H+RG++++ + ++AA A + + LT WGWR PF I Sbjct: 145 FLVEHAP-HRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLI 203 Query: 222 NVVALFSRLRLVSAPEYAKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVT 281 ++ R L APE+ + E A + + Q ++IGA + + + +L Sbjct: 204 APAGIYIRRHLDEAPEFK---ESSEKTNAPLRDTFAHQKMRLLIGAGSVIMATVSVYLSL 260 Query: 282 VFPLSWIKLYSLREATDFLVVQIWGGLIMAVGIVISGLIADRFGRRN-TLGSMALLIAIL 340 P +K L + F + + GLIM +G + G ++D+ GR + S AL I + Sbjct: 261 YIPTYAVKQLGLPAWSSFAAMSV-AGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLT 319 Query: 341 SAFVPTLMNGGEAGQNTFILIGFALLGLSYGQAAGAVTAN-FKARYRYTGAALTSDLAWL 399 L N Q + +L Y A A+ A+ F R TG +L ++A Sbjct: 320 YPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIAVT 379 Query: 400 VGAGFAPLVALGLSAHFGMAY-VSFYLLSGAVGSL-AALSLNRALEAR 445 + GFA L+ L G VS++++ GAV SL A+LS L R Sbjct: 380 IFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSARLVLRLR 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,402,045 Number of extensions: 62800 Number of successful extensions: 384 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 357 Number of HSP's successfully gapped: 11 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)