BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0133.p
         (442 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba...   874   0.0  
gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba...    56   6e-09

>gi|300311782|ref|YP_003775874.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 442

 Score =  874 bits (2257), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 439/442 (99%), Positives = 441/442 (99%)

Query: 1   MKNHHYPEQLYLLPYIGKWTLIACLIAALAGSASAFFLFALDWATHWRERHAWIIWLLPL 60
           MKNHHYPEQLYLLPYIGKWTLIACLIAALAGSASAFFLFALDWATHWRERHAWIIWLLPL
Sbjct: 1   MKNHHYPEQLYLLPYIGKWTLIACLIAALAGSASAFFLFALDWATHWRERHAWIIWLLPL 60

Query: 61  GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGASVGREG 120
           GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGASVGREG
Sbjct: 61  GGFAVGWLYLHTGKPVEAGNNLLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGASVGREG 120

Query: 121 TAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMR 180
           TAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMR
Sbjct: 121 TAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLAIGRMR 180

Query: 181 YEAIFPCFVAAIAADQVGLAWGVHHTHYAMSASAPMALWSVAAVIAAGVVFGLAGMIFAN 240
           YEAIFPCFVAAIAADQVGLAWGVHHTHYAM+ASAPMALWSVAAVIAAGVVFGLAGMIFAN
Sbjct: 181 YEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGLAGMIFAN 240

Query: 241 STHTLSAFMKRHVSYAPLRPFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPLAPWDF 300
           STHTLSAFMKRHVSYAPLRPFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPLAPWDF
Sbjct: 241 STHTLSAFMKRHVSYAPLRPFIGGFIVALAVWAVGTHRYIGLGIPVIVEAFQQPLAPWDF 300

Query: 301 LGKMVFTVTSLGSGFKGGEVTPLFYIGATLGNALAPLLHLPFPLLAGIGFVAVFAGAANT 360
           LGKMVFTVTSLGSGFKGGEVTPLFYIGATLGNALAPLLHLPFPLLAGIGFVAVFAGAANT
Sbjct: 301 LGKMVFTVTSLGSGFKGGEVTPLFYIGATLGNALAPLLHLPFPLLAGIGFVAVFAGAANT 360

Query: 361 PLASTIMAIELFGPQIGPFAALACVVAYLFSGHTGIYHAQRVGQAKLGLLPKGLRIGEVG 420
           PLASTIMAIELFGPQIGPFAALACVVAYLFSGHTGIYHAQRVGQAKLGLLPKGLRIGEVG
Sbjct: 361 PLASTIMAIELFGPQIGPFAALACVVAYLFSGHTGIYHAQRVGQAKLGLLPKGLRIGEVG 420

Query: 421 GYRRKLSAAPAEQASDLDQRRK 442
           GYRRKLSAAPAEQAS+LDQ RK
Sbjct: 421 GYRRKLSAAPAEQASELDQHRK 442


>gi|300311435|ref|YP_003775527.1| chloride channel protein
           [Herbaspirillum seropedicae SmR1]
          Length = 555

 Score = 55.8 bits (133), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 51/178 (28%), Positives = 81/178 (45%), Gaps = 2/178 (1%)

Query: 57  LLPLGGFAVGWLYLHTGKPVEAGNN-LLIDEIHDPRKVIPLRMAPLVLLGTVASHLFGAS 115
           LLP  G  V  L+L   K   AGN    ++ +      IP+R   L  + ++AS   G S
Sbjct: 51  LLPTCGGVVAGLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGS 110

Query: 116 VGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEVLA 175
           +GREG  VQ+    A  +           R+L+  G +AG +S +  P+AGA F  E++ 
Sbjct: 111 IGREGPMVQLSALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIV- 169

Query: 176 IGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMSASAPMALWSVAAVIAAGVVFGL 233
           +G +  E+  P  VA++ A+        +   Y M     +  W V   +  GV+ G+
Sbjct: 170 LGSLVMESFGPVVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGV 227


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.327    0.141    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,351,953
Number of extensions: 56053
Number of successful extensions: 268
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 262
Number of HSP's successfully gapped: 5
Length of query: 442
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 354
Effective length of database: 1,203,190
Effective search space: 425929260
Effective search space used: 425929260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)