BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0126.p (808 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [He... 1641 0.0 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 108 2e-24 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 93 7e-20 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 93 9e-20 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 93 1e-19 >gi|300311789|ref|YP_003775881.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 808 Score = 1641 bits (4249), Expect = 0.0, Method: Compositional matrix adjust. Identities = 806/808 (99%), Positives = 807/808 (99%) Query: 1 MTIRMRITLLVILTFIAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 MTIRMRITLLVILTFIAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV Sbjct: 1 MTIRMRITLLVILTFIAIAGIGGYAMYQSRSSAVEVRSVTEGVMPSVLASADLMGQLKDV 60 Query: 61 QLAAMVMVTENDNQLVAQENEKLAEKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY 120 QLAAMVMVTENDNQLVAQENEKLAEKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY Sbjct: 61 QLAAMVMVTENDNQLVAQENEKLAEKKAVLEKALAAQAQQAEGVAQRGLIEQAKESLDNY 120 Query: 121 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL Sbjct: 121 FGAIDTTAKFKLAGKNEIAQAQFFGGVMQYQTELEGIVNTLRIEKNRFKDNAIASLNGKL 180 Query: 181 AQTTTTISIVTVMAVLLLGGIGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE 240 AQTTTTISIVTVMAVLLLGGIGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE Sbjct: 181 AQTTTTISIVTVMAVLLLGGIGFLLYRQITGPISRMAAMMGEIATSQDFTRRLPVQRMDE 240 Query: 241 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVEGNRIHPEYSAFLETV 300 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVEGNRIHPEYSAFLETV Sbjct: 241 VGHSIVAFNGMVEKIEEASAQVRQKTADMQAMLQNIPQGILTIVEGNRIHPEYSAFLETV 300 Query: 301 FETTDIAGRDVMELVFADTQLGADTLSQIEAIGGACIGEDVMNFEFNAHLMVGEIEKTMA 360 FETTDIAGRDVMELVFADTQLGADTLSQIEAIGGACIGEDVMNFEFNAHLMVGEIEKTMA Sbjct: 301 FETTDIAGRDVMELVFADTQLGADTLSQIEAIGGACIGEDVMNFEFNAHLMVGEIEKTMA 360 Query: 361 DGSKKILDLSWSPITDEADTVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVTPA 420 DGSKKILDLSWSPITDEADTVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVTPA Sbjct: 361 DGSKKILDLSWSPITDEADTVIRLMLCVRDVTELRQLAAEANEQKRELEMIGEILAVTPA 420 Query: 421 KFAEFISSARRFLDENEQIVHQNPEATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 KFAEFISSARRFLDENEQIVHQNPEATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT Sbjct: 421 KFAEFISSARRFLDENEQIVHQNPEATAFAIAKLFRNMHTIKGNGRTYGLLHLANVVHHT 480 Query: 481 EQHYDDLRHPASGAVWRQEELLRQLAQVREIIDRYARINDVTLGRGVAAAGEQTLPGYAR 540 EQHYDDLRHPASGAVWRQEELLRQLAQVREIIDRYARINDVTLGRGVAAAGEQTLPGYAR Sbjct: 481 EQHYDDLRHPASGAVWRQEELLRQLAQVREIIDRYARINDVTLGRGVAAAGEQTLPGYAR 540 Query: 541 HVITIDRKHIEQSLERLENVNTANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSLPA 600 HVITIDRKHIEQSLERLENVNTANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSLPA Sbjct: 541 HVITIDRKHIEQSLERLENVNTANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSLPA 600 Query: 601 LAADLGKAAPVVQIEDNGYVVHSFAVPLLQNVFMHLIRNAMDHGLESSEERLAKGKPEAG 660 LAADLGKAAPVVQIEDNGYVVHSFA PLLQNVFMHLIRNAMDHGLESSEERLAKGKPEAG Sbjct: 601 LAADLGKAAPVVQIEDNGYVVHSFAAPLLQNVFMHLIRNAMDHGLESSEERLAKGKPEAG 660 Query: 661 VIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARLILQPGFS 720 VIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARLILQPGFS Sbjct: 661 VIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARLILQPGFS 720 Query: 721 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSFQIIVMLPENVAVEVD 780 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSFQIIVMLPENVAVEVD Sbjct: 721 TRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSFQIIVMLPENVAVEVD 780 Query: 781 GIDVPYPMGGQETLQPVVLPQGGDSQVA 808 GIDVPYPMGGQET+QPVVLPQGGDSQVA Sbjct: 781 GIDVPYPMGGQETVQPVVLPQGGDSQVA 808 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 108 bits (270), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 96/301 (31%), Positives = 145/301 (48%), Gaps = 33/301 (10%) Query: 503 RQLAQVREIIDRYARINDVTLGRGVAAAGEQTLPGYARHVITIDRKHIEQSLERLENVNT 562 R L + R ++ +I L + + GE + G + +I RK Q E + ++T Sbjct: 321 RALDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKD-SQFEEATQAIDT 379 Query: 563 ANLHELVAVRNDVRRTLRLLGTETIQEVLAPVTDSLPALAADLGKAAPVVQIEDNGYVVH 622 L+ D TLR++ I EV + +A DLGK +V + + Sbjct: 380 -----LMEQIRDAALTLRMV---QINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDK 431 Query: 623 SFAVPLLQNVFMHLIRNAMDHGLESSEERLAKGKPEAGVIKLEMGVMEDMFQMALSDDGR 682 S V L + MH++RNA+DHG+E + ERLA GKP ++L + + DDGR Sbjct: 432 SM-VERLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGR 490 Query: 683 GLALERVRQIGIEKHLITVDQALSDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFV 742 G+ E + + + L + D LSDAEI R + +PGFST +VTE+SGRGVGMD V + Sbjct: 491 GMDREAILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNI 550 Query: 743 TREHGQIEIRFTDTEEG-------------APFRSFQIIV-----MLPENVAVEVDGIDV 784 HG++ I DT G A FQ++V ++P ++ VE ID+ Sbjct: 551 EALHGEVSI---DTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVEC--IDM 605 Query: 785 P 785 P Sbjct: 606 P 606 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 93.2 bits (230), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 57/150 (38%), Positives = 85/150 (56%), Gaps = 7/150 (4%) Query: 629 LQNVFMHLIRNAMDHGLESSEERLAKGKPEAGVIKLEMGVMEDMFQMALSDDGRGLALER 688 L HL+RNA HG+ES+E+RLA+GKP G + ++ + + +SDDGRGL LE Sbjct: 457 LMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEA 516 Query: 689 VRQIGIEKHLITVDQALSDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQ 748 +RQ +E + +A S A +A LI QPG ST +V+ ++GRG+GMD V V G Sbjct: 517 IRQKALELGAAGLAEA-SQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGW 575 Query: 749 IEIRFTDTEEGAPFRSFQIIVMLPENVAVE 778 + + P + Q + LP ++AV+ Sbjct: 576 MRV------HSQPGQGVQFTLYLPLSLAVQ 599 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 92.8 bits (229), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 74/214 (34%), Positives = 111/214 (51%), Gaps = 35/214 (16%) Query: 601 LAADLGKAAPVVQIEDNGYVVHSFAVPL-------LQNVFMHLIRNAMDHGLESSEERLA 653 LA LGK Q+E +V H A L + + HL+RN++DHG+E + R A Sbjct: 386 LAHKLGK-----QVE---FVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARRA 437 Query: 654 KGKPEAGVIKLEMGVMEDMFQMALSDDGRGLALERVRQIGIEKHLITVDQALSDAEIARL 713 GK EAG + L + ++DDG GL E++ ++ + D LSDAE+ +L Sbjct: 438 SGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQGMPVTDN-LSDAEVWQL 496 Query: 714 ILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIRFTDTEEGAPFRSF--QIIVML 771 I +PGFST +VT+VSGRGVGMD V + G ++IR + + F I + L Sbjct: 497 IFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSS--------KGFGTTISISL 548 Query: 772 PENVAVEVDGIDVP-------YPMGG-QETLQPV 797 P +A+ +DG+ + P+G E+LQP+ Sbjct: 549 PLTLAI-LDGMSIRIGEEVYILPLGYVVESLQPL 581 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 92.8 bits (229), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 62/157 (39%), Positives = 94/157 (59%), Gaps = 4/157 (2%) Query: 635 HLIRNAMDHGLESSEERLAKGKPEAGVIKLEMGVMEDMFQMALSDDGRGLALERVRQIGI 694 HL+RNA+DHG+ESS +R A GKPE GVI+LE M + ++DDG G+ +E+VR + Sbjct: 357 HLLRNAVDHGIESSAQRRAAGKPEQGVIRLEARHSAGMLLIEVADDGAGIDIEQVRSAVV 416 Query: 695 EKHLITVDQA--LSDAEIARLILQPGFSTRSEVTEVSGRGVGMDAVLDFVTREHGQIEIR 752 + L + A LS+AE+ +L P FS R VTE+SGRGVG+D V D + + G I I Sbjct: 417 ARGLSNQETAERLSEAELLEFLLLPSFSLRDTVTEISGRGVGLDVVADMLKQVRGTIRI- 475 Query: 753 FTDTEEGAPF-RSFQIIVMLPENVAVEVDGIDVPYPM 788 T +G+ F + + + ++ V++ G +P+ Sbjct: 476 TTQAGQGSRFLMQLPLTLSVIRSLLVDIAGEPYAFPL 512 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,303,836 Number of extensions: 95896 Number of successful extensions: 447 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 455 Number of HSP's successfully gapped: 11 Length of query: 808 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 715 Effective length of database: 1,179,515 Effective search space: 843353225 Effective search space used: 843353225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)