BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0125.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 529 e-152 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 140 2e-34 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 135 2e-33 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 129 3e-31 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 129 3e-31 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 529 bits (1363), Expect = e-152, Method: Compositional matrix adjust. Identities = 260/261 (99%), Positives = 261/261 (100%) Query: 1 MSALNPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP 60 MSALNPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP Sbjct: 1 MSALNPSRLQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPP 60 Query: 61 ERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGIFAEGNVTEVSPEDWHRVQATN 120 ERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAG+FAEGNVTEVSPEDWHRVQATN Sbjct: 61 ERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATN 120 Query: 121 VNGVFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 VNGVFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR Sbjct: 121 VNGVFYGAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 Query: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF Sbjct: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 Query: 241 VNGVNLPVDGGLMASNGQPPQ 261 VNGVNLPVDGGLMASNGQPPQ Sbjct: 241 VNGVNLPVDGGLMASNGQPPQ 261 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 87/255 (34%), Positives = 132/255 (51%), Gaps = 5/255 (1%) Query: 6 PSRLQRF--EGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERT 63 P+ LQ F EGK+ ++TG++ GIG A AR + GA ++L RDA L V L E Sbjct: 9 PAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGH 68 Query: 64 AARET--DVSHHEQVRQLVDFAIERFGQLDVLVSDAGIFAEGNVTEVSPEDWHRVQATNV 121 E+ D + E+V+ V G + +LV++AGI + E DWH + +TN+ Sbjct: 69 TIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNL 128 Query: 122 NGVFYGAREALPHL-EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGR 180 N VF+ A+ H+ + G I+N+ SV ++ Y A+KGAV T+ MA+D+G+ Sbjct: 129 NSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGK 188 Query: 181 KGVRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASF 240 G+++N + P T M + +D D R R D ++ FLAS + F Sbjct: 189 YGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRF 248 Query: 241 VNGVNLPVDGGLMAS 255 VNG L VDGG+ A+ Sbjct: 249 VNGHILYVDGGVTAT 263 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 135 bits (341), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 86/244 (35%), Positives = 122/244 (50%), Gaps = 10/244 (4%) Query: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETDVS 71 +GK +VTG GIG A AR+ GARV+ A A + E +V+ Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFA----HEEFAGSEVTLVEMNVA 63 Query: 72 HHEQVRQLVDFAIERFGQLDVLVSDAGIFAEGNVTEVSPEDWHRVQATNVNGVFYGAREA 131 E VR A+ QLD++V+ AGI G E+ E + +V A N+ G A Sbjct: 64 SEESVRA----ALAGLTQLDIVVNCAGIIKRG--AELETEVFEQVIAVNLTGTMRVCAAA 117 Query: 132 LPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVCPS 191 L ++RGCIVN AS+ + Y+ASKG V LT+++A+ + G+R+NAV P Sbjct: 118 RELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 192 LTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 T +T + DDP R LGR P ++ V+ FL SP ASF+ G +PVDGG Sbjct: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 Query: 252 LMAS 255 + + Sbjct: 238 YLVA 241 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 129 bits (323), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 86/249 (34%), Positives = 136/249 (54%), Gaps = 7/249 (2%) Query: 10 QRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPE--RTAARE 67 R + KV IVTG SG GE A+ ++ EGA +++AD A +V + + R + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 68 TDVSHHEQVRQLVDFAIERFGQLDVLVSDAGIFAEGN-VTEVSPEDWHRVQATNVNGVFY 126 DVS + QL+ A+E FG+LD++V++AG + EV +++ RV A NV +F Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 127 GAREALPHLEKTRG-CIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRI 185 A+ +P+ + G +N+AS +G+ L+ YN SKGAV +++MA + G +R+ Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 186 NAVCPSLTHTAMTADMA---DDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVN 242 N V P ++ T + ++ D P KF I +GR + P +IA +L S +A FV Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 Query: 243 GVNLPVDGG 251 GV + VDGG Sbjct: 241 GVCIEVDGG 249 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 129 bits (323), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 89/249 (35%), Positives = 130/249 (52%), Gaps = 11/249 (4%) Query: 10 QRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETD 69 ++ GK+ +VTG SGIG ATA+ + +GA+V + R A L S+ T R D Sbjct: 3 KQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIR-AD 61 Query: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAG---IFAEGNVTEVSPEDWHRVQATNVNGVFY 126 S ++ + + G+LD+L ++AG + G +TE E + R+ TNV GV + Sbjct: 62 ASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITE---EHFDRIFGTNVRGVLF 118 Query: 127 GAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRIN 186 ++ALP L I+ ++ S L N S Y+ASK AV N R+ ALD + +R+N Sbjct: 119 TVQKALPLLVDGASVILTSSTTSVLGT-ANFSVYSASKAAVRNFARSWALDLKDRAIRVN 177 Query: 187 AVCPSLTHTAMTADMADDPP---LLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 V P T ++ D L D A ++ LGR +P EI + FLAS ASFVNG Sbjct: 178 VVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNG 237 Query: 244 VNLPVDGGL 252 + L VDGG+ Sbjct: 238 IELFVDGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 789,760 Number of extensions: 31646 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 48 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)