BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0030.0122.p
         (260 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase [Herb...   516   e-148
gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase [Herb...    75   6e-15

>gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 260

 Score =  516 bits (1330), Expect = e-148,   Method: Compositional matrix adjust.
 Identities = 260/260 (100%), Positives = 260/260 (100%)

Query: 1   MRSARLSERQTAPAPADTELVLSADAPVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAA 60
           MRSARLSERQTAPAPADTELVLSADAPVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAA
Sbjct: 1   MRSARLSERQTAPAPADTELVLSADAPVPGRDPWPLTVATYNIHGAVGTDGRFAPERVAA 60

Query: 61  VLDELQADVVALQEVPLGGADQVDVLALLRAHTGMFAAEGPTMESPARRYGNAVLSRYPI 120
           VLDELQADVVALQEVPLGGADQVDVLALLRAHTGMFAAEGPTMESPARRYGNAVLSRYPI
Sbjct: 61  VLDELQADVVALQEVPLGGADQVDVLALLRAHTGMFAAEGPTMESPARRYGNAVLSRYPI 120

Query: 121 LATESIDLSFGSREPRGALDADIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTNDA 180
           LATESIDLSFGSREPRGALDADIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTNDA
Sbjct: 121 LATESIDLSFGSREPRGALDADIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTNDA 180

Query: 181 PVILLGDVNEWFMWGRTLRWLVGHFEAAPAPPTFPSRWPVFALDRIWISPRRRLQHLAVH 240
           PVILLGDVNEWFMWGRTLRWLVGHFEAAPAPPTFPSRWPVFALDRIWISPRRRLQHLAVH
Sbjct: 181 PVILLGDVNEWFMWGRTLRWLVGHFEAAPAPPTFPSRWPVFALDRIWISPRRRLQHLAVH 240

Query: 241 RSALARVASDHLPLLAHIAG 260
           RSALARVASDHLPLLAHIAG
Sbjct: 241 RSALARVASDHLPLLAHIAG 260


>gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 265

 Score = 75.1 bits (183), Expect = 6e-15,   Method: Compositional matrix adjust.
 Identities = 80/265 (30%), Positives = 112/265 (42%), Gaps = 47/265 (17%)

Query: 36  LTVATYNIH-GAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGADQVDVLALLRAHT- 93
           L VATYNIH G     GR     +   L +L  D++ LQEV      + D+ AL  A   
Sbjct: 3   LRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEV----QGRHDLNALRHASNW 58

Query: 94  ------------GMFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDA 141
                          AA G         +GNA++SR+ I +  + D+S  + E RG L  
Sbjct: 59  PQQGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHC 118

Query: 142 DIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTN---DAPVILLGDVNEWF-MWGRT 197
            +   G  +     HLGL    RR QI  L+QA  T+   DAP+++ GD N+W     RT
Sbjct: 119 VVPVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRT 178

Query: 198 LRWLVGHFE----------------------AAPAPP--TFPSRWPVFALDRIWISPRRR 233
           L   +G  E                      +AP  P  TFP+  P   LDRI++   R 
Sbjct: 179 LYQNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRV 238

Query: 234 LQHLAVHRSALARVASDHLPLLAHI 258
                +     AR+ SDH P++  +
Sbjct: 239 ETAEVLKDGPWARL-SDHAPIVTEL 262


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.136    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 937,642
Number of extensions: 44185
Number of successful extensions: 161
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 158
Number of HSP's successfully gapped: 2
Length of query: 260
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 177
Effective length of database: 1,226,865
Effective search space: 217155105
Effective search space used: 217155105
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)