BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0117.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 930 0.0 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 420 e-118 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 300 2e-82 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 297 1e-81 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 202 4e-53 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 930 bits (2404), Expect = 0.0, Method: Compositional matrix adjust. Identities = 472/476 (99%), Positives = 474/476 (99%) Query: 1 MTALVLAALLGGCAVGPDYVRPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLN 60 MTALVLAALLGGCAVGPDY RPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLN Sbjct: 35 MTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLN 94 Query: 61 ALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQRLGNS 120 ALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQRLGNS Sbjct: 95 ALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQRLGNS 154 Query: 121 YSAGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQK 180 YS GLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQK Sbjct: 155 YSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQK 214 Query: 181 DLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIA 240 DLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIA Sbjct: 215 DLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIA 274 Query: 241 MLIGKAPAQFSLPALPPASASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERL 300 MLIGKAPAQFSLPALPPA+ASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERL Sbjct: 275 MLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERL 334 Query: 301 AAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAETLFDGGARS 360 AAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAE+LFDGGARS Sbjct: 335 AAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAESLFDGGARS 394 Query: 361 ARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAM 420 ARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAM Sbjct: 395 ARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAM 454 Query: 421 RQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGWQAGRDAP 476 RQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGWQAGRDAP Sbjct: 455 RQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGWQAGRDAP 510 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 420 bits (1079), Expect = e-118, Method: Compositional matrix adjust. Identities = 248/482 (51%), Positives = 329/482 (68%), Gaps = 28/482 (5%) Query: 1 MTALVLAAL-LGGCAVGPDYVRPALDL-PQAYKETGPWKLAAPRQIDDHQPWWEAYGDPV 58 + A+++A+L L C+VGPDYVRP +D P +KE WK A PR + WWE +GDP Sbjct: 12 LLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMFGDPQ 71 Query: 59 LNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQR-- 116 LNAL+ Q + +NQ + A A +R A A + +R++ PT+ A AGASR + + G Sbjct: 72 LNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGASSSN 131 Query: 117 ---------LGNSYSAGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLA 167 + NSY+ L A+WEAD+WG++RR E+ +A+AQASAA LAAARLS QA +A Sbjct: 132 AASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVA 191 Query: 168 QDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLID 227 Q+YLQLRV D Q+ L TV AY RSLQ++ +QY AG +SDV QA+TQL++AQA +D Sbjct: 192 QNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASALD 251 Query: 228 LDDTRNQLEHAIAMLIGKAPAQFSLPALPPASASDSAIDANAARLQGSLPQIPVGLPSEL 287 TR Q+EHAIA+L+GK PAQF+LP P A+ + P +P G+PS L Sbjct: 252 NGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVA---------------PPVPQGVPSML 296 Query: 288 LERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGS 347 LERRPD+A AER A+ANA IGVA+AAYFP+LTLSASGGY S FA+ F P RVWSLG Sbjct: 297 LERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGP 356 Query: 348 ALAETLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQ 407 LA+T+FDGGAR+A D A+A YD VA YKQTVL QEVED L+ LR+L+QE+ VQ + Sbjct: 357 QLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGE 416 Query: 408 AVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGG 467 A+Q+A+ A L QY+AGTV+Y++V+T QA +L+ + +++ +L R++ ASV L KA GG Sbjct: 417 ALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGG 476 Query: 468 GW 469 GW Sbjct: 477 GW 478 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 300 bits (767), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 197/479 (41%), Positives = 274/479 (57%), Gaps = 36/479 (7%) Query: 12 GCAVGPDYVRPALDLPQAYKETGP----------WKLAAPRQIDDHQPWWEAYGDPVLNA 61 GC+V P Y RP++D P A+KE P WK A P + WW+ +GD LNA Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 62 LMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTN--TNGV----- 114 L D A ANQ ++ AAA+ QA+A ARA +P + A GA+R + + + G+ Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 115 QRLGNSYSAGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLR 174 R Y A +N S+E DL+GR+ +A A+AQ S A + L++QA +AQ Y +R Sbjct: 150 TRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVR 209 Query: 175 VTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQ 234 D LYR TV SL+L +++ G DVA+A +L +AQ++ + + R Sbjct: 210 ELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRAT 269 Query: 235 LEHAIAMLIGKAPAQFSLPALPPASASDSAIDANAARLQGSLPQIPVGLPSELLERRPDI 294 EHA+A+L+G+ PA+FSLP P RL + +P GLPS LLERRPDI Sbjct: 270 AEHALAILLGRTPAEFSLPPQP------------LTRLAVT---VPPGLPSALLERRPDI 314 Query: 295 ANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLG----SALA 350 A AER AAANA +GVA++A+FP+L L+ GY S +LFN SR + LG +AL+ Sbjct: 315 AAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALS 374 Query: 351 ETLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQ 410 LFDGG R A D A A+Y+ VA Y+ TVL +EVED L+ LR+LDQ++ Q +AV Sbjct: 375 MPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVA 434 Query: 411 SAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGW 469 ++ A +L+ QY G V+Y SV+ L QR +VTL G + ++V LI+A GGGW Sbjct: 435 ASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 297 bits (761), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 192/470 (40%), Positives = 269/470 (57%), Gaps = 26/470 (5%) Query: 12 GCAVGPDYVRPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLNALMDQANAANQ 71 GCA GPDY RP +D+P A+ PW+ P PWWE +GD LNAL QA A NQ Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 72 TIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTN---------GVQRLGNSYS 122 T+ A A+ QA+A V A+ P + A A+R++ + N + N ++ Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFSTVQNDFA 137 Query: 123 AGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQKDL 182 G + S+E D +GR++R EA A A+ SAA L RL + A LA +Y LR D++ D+ Sbjct: 138 LGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDV 197 Query: 183 YRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIAML 242 RR +A ++L+L+T +++ G D+AQ + L + Q+ L R Q EHAIA L Sbjct: 198 VRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATL 257 Query: 243 IGKAPAQFSL-PALPPASASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERLA 301 G FS+ P+L P + P IPVG+PS++LERRPDIA AER Sbjct: 258 TGTPAPAFSITPSLTPITP----------------PAIPVGVPSDVLERRPDIAAAERAM 301 Query: 302 AAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAETLFDGGARSA 361 AAANA IGVA AAY+P+ L S G +S +A LFN PS +WSLG + +++LFDGG A Sbjct: 302 AAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRA 361 Query: 362 RNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAMR 421 D + A Y+A VA Y++TVL +QEVED ++ L L++ A+ SA+ +A Sbjct: 362 GVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASS 421 Query: 422 QYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGWQA 471 +YE G YL V+T Q + L ++R A L+G++L+ SV LIKA GG WQ+ Sbjct: 422 RYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQS 471 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 202 bits (515), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 162/467 (34%), Positives = 253/467 (54%), Gaps = 29/467 (6%) Query: 13 C-AVGPDYVRPALDLPQAYKETGPWKLAAPRQIDDHQ----PWWEAYGDPVLNALMDQAN 67 C ++ P Y RPA + Y + GP AA + ++ PW + + DP L AL+ QA Sbjct: 25 CQSLAPHYQRPAAPVAAVYPD-GPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQAL 83 Query: 68 AANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQT--NTNGVQR--LGNSYSA 123 ++ ++ AA + ++AQA + A L+PTIGAQAG R++T + N +R +G++Y A Sbjct: 84 ENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQA 143 Query: 124 GLN-ASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQKDL 182 GL +SWE DLWGR+R +A +A+AS A+ +A LS+ A +A +L+L D + ++ Sbjct: 144 GLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEI 203 Query: 183 YRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIAML 242 +T+A+ S ++ + + E G R ++ Q ET L AQA L R Q +A+ +L Sbjct: 204 ANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLL 263 Query: 243 IGKAPAQFSLPALPPASASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERLAA 302 +G P + P + + +DA A LQ GLP+ LL RRPD+A AE Sbjct: 264 VGS-------PVVLPVGQARAGLDAVFAPLQP-------GLPALLLTRRPDVAAAEHRLM 309 Query: 303 AANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAETLFDGGARSAR 362 AANANIG ARAA+FP+++L+ + G S + LF S W+ ++ +F G R Sbjct: 310 AANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAG--RLR 367 Query: 363 NDAAVAAY--DAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAM 420 N+ A++ D AVA Y++TV +EV D L+ + L Q+ + AV+ + RL+ Sbjct: 368 NNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSA 427 Query: 421 RQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGG 467 +Y+ G +L V+ Q L+ Q+ V + L +SVAL A GG Sbjct: 428 LRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.125 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,006,238 Number of extensions: 28026 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 18 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)