BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0116.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 796 0.0 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 288 4e-79 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 157 1e-39 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 135 5e-33 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 129 4e-31 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 796 bits (2055), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/403 (99%), Positives = 400/403 (99%) Query: 1 MSTSPTPPRRSRLAIALAAALLMAGIGGWTHLQRASAADQPQTKAPPPVSVTLASVQERD 60 MSTSPTPPRRSRLAIALAAALLMAGIGGWTHLQRASAADQPQ KAPPPVSVTLASVQERD Sbjct: 1 MSTSPTPPRRSRLAIALAAALLMAGIGGWTHLQRASAADQPQAKAPPPVSVTLASVQERD 60 Query: 61 LPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAE 120 LPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAE Sbjct: 61 LPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAE 120 Query: 121 AQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKV 180 AQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKV Sbjct: 121 AQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKV 180 Query: 181 QLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRA 240 QLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRA Sbjct: 181 QLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRA 240 Query: 241 QRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVN 300 QRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVN Sbjct: 241 QRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVN 300 Query: 301 ARLQMRSHGNALTVPAAAIQRNQQGTYVYTIDQEDQARMQPVKVERIQDGIAVLAAGNAV 360 ARLQMRSHGNALTVPAAAIQRNQQGTYVY IDQEDQARMQPVKVERIQDGIAVLAAGNAV Sbjct: 301 ARLQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVERIQDGIAVLAAGNAV 360 Query: 361 QAGQRVVIDGQYKLKPGSRIVESKPQPAKAKAEAEAKAKGNAK 403 QAGQRVVIDGQYKLKPGSRIVESKPQPAKA+A AEAKAKGNAK Sbjct: 361 QAGQRVVIDGQYKLKPGSRIVESKPQPAKAEANAEAKAKGNAK 403 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 288 bits (738), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 158/341 (46%), Positives = 223/341 (65%), Gaps = 10/341 (2%) Query: 41 PQTKAPPPVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKK 100 PQT PV V A +Q D+ +L G+G VT A+ V+ R+DGQL K+ ++EG+ VK Sbjct: 29 PQTM---PVGVAKARLQ--DVNVFLNGLGAVTPTATATVRARVDGQLMKLHYKEGQVVKA 83 Query: 101 GQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKAL 160 G LLA+ID R+LQA L QAE Q A+D A L +ARLDL+RY TL +QD+ QQ+DTQ AL Sbjct: 84 GDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLKRYQTLLAQDSIASQQVDTQVAL 143 Query: 161 VAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQI 220 V Q E VK DE VA A++QLS+T + APISGR+G R D GN V +D NGLV+I Q+ Sbjct: 144 VKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLRQADVGNNVTTSDTNGLVIITQL 203 Query: 221 DPITLVFTLPEEAVAGI-NRAQRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGT 279 PIT +F++PE+ + + + Q + P Q A+ R+ + LA G L+ +DN ++ T GT Sbjct: 204 QPITAIFSIPEDNIPKVLQQLQSGRKLPAQ--AWDREQKNKLADGVLLTIDNVVDATTGT 261 Query: 280 VQLKARFDNPDHKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQGTYVYTIDQEDQARM 339 V+LKA+F N D+ L+P Q+VN RLQ+ + A +P AAIQR +G +VY + + + Sbjct: 262 VKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTAAIQRGSKGLFVYVVKDDSSVTV 321 Query: 340 QPVKVERIQDGIAVLAAGNAVQAGQRVVIDGQYKLKPGSRI 380 +PVK +QD + V+ G V AG+ VVIDG +L+ G+++ Sbjct: 322 RPVKTGPVQDDLTVITDG--VSAGETVVIDGIDRLREGAKV 360 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 157 bits (398), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 129/411 (31%), Positives = 203/411 (49%), Gaps = 40/411 (9%) Query: 10 RSRLAIALAAALLMAGIGGWTHLQRASAADQPQTKAPPPVSVTLASVQERDLPQYLAGV- 68 R + +A+AAAL+ G Q+ +A P PP V++ ++ PQ +A Sbjct: 9 RLTMTVAIAAALVACG-------QKPAAQGGPM----PPSEVSVVTIA----PQRVAITN 53 Query: 69 ---GTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAK 125 G V A V+ R+ G ++K F+EG +VK+GQ+L +ID +A L A+A A+ Sbjct: 54 ELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLAR 113 Query: 126 DSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYT 185 A L L QRY L +A ++Q+ D A Q EA V +A V A + L Y Sbjct: 114 AQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYA 173 Query: 186 TINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAHQ 245 T+ +PISGR+G LV G +V DA L ++ Q+DP+ + T + + +A A Q Sbjct: 174 TVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQ 233 Query: 246 ------QPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYV 299 V+ + Q GKL+ D ++ G+V L+A F NP L PG YV Sbjct: 234 LKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYV 293 Query: 300 NARLQMRSHGNALTVPAAAIQRNQQGTYVYTIDQEDQARMQPVKVERIQ-DGIAVLAAGN 358 A+++ AL VP A+ R+ G+ V ++ E++ +PVK + + VL + Sbjct: 294 RAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVL---D 350 Query: 359 AVQAGQRVVIDGQYKLKPGSRI--VESKP---------QPAKAKAEAEAKA 398 ++ G RV+++G K++PG+ + V+ P QPAK +A A+ KA Sbjct: 351 GLKEGDRVIVEGLQKVRPGATVKPVQWNPAGAPAQASGQPAKQEASAQPKA 401 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 135 bits (341), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 122/395 (30%), Positives = 184/395 (46%), Gaps = 38/395 (9%) Query: 3 TSPTPPRRSRLAIA---LAAALLMAGIGGWTHLQRASAADQPQTKAPPPVSVTLASVQER 59 T+ TP R+ R + A LA +L++ + + + DQP+ A V + Q + Sbjct: 4 TASTPRRQGRTSSATARLALSLILVAVLAAC----SRSGDQPKPTAQAEVGYVVVKAQRQ 59 Query: 60 DLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQA 119 L L G TA + ++ ++ G ++K F+EG VK GQ L QID + QA A Sbjct: 60 ALTTELPG--RTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSA 117 Query: 120 EAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAK 179 A K ATL +++L +RY L DA ++Q D ++ + Q +A V+ +A V A Sbjct: 118 RASLLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAA 177 Query: 180 VQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFT--------LPE 231 + L YT I +PISGR+ V PG +V A+ L + QIDPI + T L Sbjct: 178 INLQYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRR 237 Query: 232 EAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDH 291 E +G R ++ + QV D GKL ++T G + L+A NPD Sbjct: 238 ELASGQIR--KSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDG 295 Query: 292 KLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQG---TYVYTIDQEDQARMQPVKVERIQ 348 L PG YV A L+ +AL P A+ R+Q G V D + Q R+ V V R Sbjct: 296 VLLPGAYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRL--VSVAR-- 351 Query: 349 DGIAVLAAGN------AVQAGQRVVIDGQYKLKPG 377 A GN ++ G ++++DG K++ G Sbjct: 352 ------AVGNNWWVSRGLKEGDKLIVDGLQKVRTG 380 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 129 bits (325), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 94/310 (30%), Positives = 153/310 (49%), Gaps = 12/310 (3%) Query: 79 VKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQ 138 V+ +++G ++ F+EG DVK GQ+L QI+ + QA + A AK A++ ARL Sbjct: 69 VRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKAS 128 Query: 139 RYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGAR 198 RY L + A +QQ D A + + EA V + A V +++ L Y ++APISGR+G Sbjct: 129 RYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKS 188 Query: 199 LVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQ 258 V PG +V A L I Q+DPI + T + + +N + L+ S + Sbjct: 189 SVTPGALVTANQTTALTTIQQLDPIYVDVT--QSSTTWLNLKEALANGQLEKSGNGAKVR 246 Query: 259 EVLATGK-------LVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNA 311 VL G+ L D +N G + L+A F NP L PG YV A LQ Sbjct: 247 LVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQG 306 Query: 312 LTVPAAAIQRNQQGTYV-YTIDQEDQARMQPVKVERIQDGIAVLAAGNAVQAGQRVVIDG 370 L VP A+ R+ G V + ++ + + + +R ++++G ++ G ++V+DG Sbjct: 307 LLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSG--LKEGDQLVVDG 364 Query: 371 QYKLKPGSRI 380 Q + G+++ Sbjct: 365 QQRASAGAKV 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.127 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,008,173 Number of extensions: 37230 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 10 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)