BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0115.p (1051 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 696 0.0 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 315 1e-86 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 291 2e-79 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 85 3e-17 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 81 4e-16 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 696 bits (1795), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/329 (99%), Positives = 329/329 (100%) Query: 1 VSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 +SVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA Sbjct: 1 MSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 Query: 61 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP 120 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP Sbjct: 61 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP 120 Query: 121 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ 180 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ Sbjct: 121 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ 180 Query: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ Sbjct: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 Query: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQ 300 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQ Sbjct: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQ 300 Query: 301 PGANVIETVGRIKAAMPRLRAAIPAAIHV 329 PGANVIETVGRIKAAMPRLRAAIPAAIHV Sbjct: 301 PGANVIETVGRIKAAMPRLRAAIPAAIHV 329 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 315 bits (807), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 164/341 (48%), Positives = 233/341 (68%), Gaps = 15/341 (4%) Query: 7 FIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMASNVAQP 66 FI RP+ T+LL +AILLVG+ + LLP++ LP+VD+PTIQV PG SP+ M S+V P Sbjct: 7 FILRPVATSLLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMTSSVTAP 66 Query: 67 LERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLPANLPNP 126 LERQF + GL QM+S S+ G++ ITLQF+L+ ++D A +VQAAINA + LP++LP P Sbjct: 67 LERQFGQMPGLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLPSDLPMP 126 Query: 127 PTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQQKPAVR 186 P + K+NPAD+PI+ ++V S +PL V D D +AQ+ISQ+SGVGLV+++G Q+PAVR Sbjct: 127 PVYSKVNPADTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGGQRPAVR 186 Query: 187 IQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQWNDMVL 246 +Q++P +A LGL+L+D+R I VNQ KG+ DG +A T+ NDQL A+++ ++++ Sbjct: 187 MQLNPRAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADEYRNLII 246 Query: 247 AWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGRSIVLAITKQPGANVI 306 A++NGAPIRV D+ VDG EN ++ AW ANT I N I +QPGANVI Sbjct: 247 AYKNGAPIRVSDVADVVDGAENVRLGAW-----ANTQPAVIVN-------IQRQPGANVI 294 Query: 307 ETVGRIKAAMPRLRAAIPAAI--HVIRS-STAPRPSAPRWK 344 V IK +P+L+A++P AI H++ +T R S K Sbjct: 295 AVVDSIKKILPQLQASLPGAIDVHILTDRTTTIRASVDDVK 335 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 291 bits (744), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 149/330 (45%), Positives = 230/330 (69%), Gaps = 14/330 (4%) Query: 1 VSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 +++S FI+RPI TTLL I I L G+ + LLPV+PLPQVD+PTI ++A +PG SPETMA Sbjct: 1 MNISLPFIQRPIATTLLTIGIALAGIVAFRLLPVSPLPQVDYPTISISASMPGASPETMA 60 Query: 61 SNVAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLP 120 + VA PLER IAG+++MTS S+ ST+ITLQFDL+R+ID AA DVQAA+NA+ LP Sbjct: 61 ATVATPLERAMGAIAGVTEMTSTSSQNSTRITLQFDLSRDIDGAARDVQAALNAARNLLP 120 Query: 121 ANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQ 180 +P+ PT+RK+NPAD+PI+I+++ S+ + + D AD+I+AQ++SQ+ GVG V+V G Sbjct: 121 TGMPSNPTYRKVNPADAPILILAMTSDTMTQGQMYDAADSIVAQKLSQVRGVGQVSVGGS 180 Query: 181 QKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQ 240 +PAVRI+++P L G+ D+R +A T N+PKG ++ + + +Y NDQ A + Sbjct: 181 SQPAVRIELNPTALNKYGIGTADVRTAVAATNANRPKGLLEDQDRNWQIYANDQAKTAAE 240 Query: 241 WNDMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGR-SIVLAITK 299 + +++++RNG P+RV+D+ + +A AG+ +NG+ S+++ I + Sbjct: 241 YAPLIVSYRNGTPVRVQDV-----AEVRDSVADVRNAGS--------SNGKPSVLIIINR 287 Query: 300 QPGANVIETVGRIKAAMPRLRAAIPAAIHV 329 QPGAN+I+TV ++ +P+L+A+IP+AI + Sbjct: 288 QPGANIIDTVDNVRELLPQLQASIPSAIDM 317 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 84.7 bits (208), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 85/264 (32%), Positives = 132/264 (50%), Gaps = 14/264 (5%) Query: 7 FIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMASNVAQP 66 FI RP+ +LAI I+L G LPV+ P + PTI VTA PG + +T+ +V Sbjct: 5 FIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDSVTSL 64 Query: 67 LERQFSLIAGLSQMTSQSTL-GSTQITLQFDLNRNIDAAALDVQAAINASTGQLPANLPN 125 +E++ + GL + SQS G QIT+ F N D AA+DVQ + +LPA + Sbjct: 65 IEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPAAVTQ 124 Query: 126 PPTFRKINPADSPIMI---MSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQQK 182 ++ + S ++ +S L + DY + +I ++ GVG + G ++ Sbjct: 125 QGV--QVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGTER 182 Query: 183 PAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVD-----GARQ-AFTVYTNDQLL 236 A+RI +DP KL L L+ D+ I GT+ G +Q A TV N QL Sbjct: 183 -AMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQLT 241 Query: 237 KAEQWNDMVL-AWRNGAPIRVKDI 259 EQ+ +++L A NGA +R++D+ Sbjct: 242 SPEQFGNIILRANANGATVRLRDV 265 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 81.3 bits (199), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 103/359 (28%), Positives = 167/359 (46%), Gaps = 51/359 (14%) Query: 7 FIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMASNVAQP 66 FI RPI ++A+ I+L GV LP+A P V PT+ + A PG +P+TM V Sbjct: 5 FIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVISL 64 Query: 67 LERQFSLIAGLSQM-TSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLPANL-P 124 +ER+ S + L +S T G+ QI++ F + + A +DVQ + A +LP + Sbjct: 65 IERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTVRQ 124 Query: 125 NPPTFRKINPADSPIMIMSVQS-----EALPLTVVNDYADNILAQQISQISGVGLVNVNG 179 N T + A S ++++S+ S + + L NDY + +++ ++ GVG V + G Sbjct: 125 NGVTVE--SAASSFLLMVSLTSVDGRYDEVRL---NDYLARNIVEELRRMEGVGRVQLFG 179 Query: 180 QQKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKG------TVDGARQAFTVYTND 233 ++ A+RI +DP KL GL++ DI I+ G V G + A + Sbjct: 180 AEQ-AMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQG 238 Query: 234 QLLKAEQWNDMVL-AWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSITNGR- 291 QL Q+ +VL A +G+ + + D+ G ++ +AFA NG+ Sbjct: 239 QLQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQS-----YAFANR--------ENGKP 285 Query: 292 SIVLAITKQPGANVIETVGRIKAAMPRLRAAIPAAIHVIRSSTAPRPSAPRWKTWSSPW 350 + V A+ PGAN + T I+A M L RPS P+ +S P+ Sbjct: 286 AGVAAVQLSPGANAVRTAAAIQARMEEL-----------------RPSLPQGMAFSLPY 327 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.341 0.140 0.535 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,561,604 Number of extensions: 44882 Number of successful extensions: 424 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 405 Number of HSP's successfully gapped: 11 Length of query: 1051 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 956 Effective length of database: 1,170,045 Effective search space: 1118563020 Effective search space used: 1118563020 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 38 (21.6 bits) S2: 136 (57.0 bits)