BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0114.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 462 e-132 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 273 8e-75 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 266 7e-73 gi|300309981|ref|YP_003774073.1| two component response regulato... 159 1e-40 gi|300312680|ref|YP_003776772.1| two component response regulato... 157 1e-39 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 462 bits (1190), Expect = e-132, Method: Compositional matrix adjust. Identities = 229/230 (99%), Positives = 230/230 (100%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA Sbjct: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 Query: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM Sbjct: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATADNSDD 230 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATAD+SDD Sbjct: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATADSSDD 230 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 273 bits (698), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 134/223 (60%), Positives = 170/223 (76%), Gaps = 2/223 (0%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 M IL++ED+ K +YL KGL E G AVDLA NG DG H+A+E DYD++VLDVMLPGLDG+ Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 V+ ALR ++ PV+ LTARD V+DRI+GL GADDYL+KPFSF EL+ R++ L RRG Sbjct: 61 QVMSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGVT 120 Query: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 +E I DLQ+D + K R G+ I LT KEF LL ++ +RQGE LS++ IA +WDM Sbjct: 121 REAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVL--EP 221 NFDS+TNVV+VAIKRLRAKIDAPF++KL+HTVR +GY+ EP Sbjct: 181 NFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMFSEEP 223 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 266 bits (681), Expect = 7e-73, Method: Compositional matrix adjust. Identities = 132/219 (60%), Positives = 166/219 (75%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 M IL+VEDE K +YL +GLTE G VD GVDG H A DY +I+LDVMLPG++G+ Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 SVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGRA 120 +LR LR+ +TPV+ LTARD VEDR+KGL GADDYL+KPF++ ELLAR++ L RRG + Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 QEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWDM 180 +E + D+ +D+L RK R G RIDLTAKEFALL ++ RR GE+LS+ IA VWDM Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWDM 180 Query: 181 NFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVL 219 NFDS+TNVV+VA++RLRAK+D FE+KLLHTV GMGYV Sbjct: 181 NFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300309981|ref|YP_003774073.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 237 Score = 159 bits (403), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 89/221 (40%), Positives = 135/221 (61%), Gaps = 3/221 (1%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 M+IL+ ED+ +++ L + L G A D NGV+ D+D+++LD+ LP + G Sbjct: 1 MRILLAEDDSVLADGLTRSLRHSGYAADCVKNGVEADSALSTQDFDLLILDLGLPKMSGL 60 Query: 61 SVLRALR-KVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 VLR LR + PV++LTA DSVE R++GL GADDY+ KPF+ EL AR++ALTRRG Sbjct: 61 EVLRRLRARNSRLPVLILTAADSVEQRVQGLDLGADDYMAKPFALSELEARVRALTRRGA 120 Query: 120 AQEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWD 179 P +R G L D + R A+ G ++L+A+E LL V+ +R G ++SK + + + + Sbjct: 121 GGGPTVIRHGPLSYDQVGRIAYIGEQMLELSARELGLLEVLLQRTGRLVSKEQLVDHLCE 180 Query: 180 MNFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLE 220 + + N +EV + RLR KI+ + + TVRG+GY LE Sbjct: 181 WGEEVSNNAIEVYVHRLRKKIEVGGVR--IATVRGLGYCLE 219 >gi|300312680|ref|YP_003776772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 157 bits (396), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 91/222 (40%), Positives = 137/222 (61%), Gaps = 8/222 (3%) Query: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 M +L+VED+ +++ L + L G V NG D L+ + V+VLD+ LPG+DGF Sbjct: 1 MNVLLVEDDPVLTDGLSRVLNSHGFTVHTVNNGNDA--LSQRFNAAVLVLDIGLPGIDGF 58 Query: 61 SVLRALRKV-KETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 VLR +R TPV++LTARD++ DR+ GL GADDYL+KPF+ EL+AR++AL RR Sbjct: 59 EVLRRMRAAGNNTPVLLLTARDTIPDRVHGLELGADDYLVKPFATPELVARIKALIRRS- 117 Query: 120 AQEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWD 179 A +PAQL +G L +D +++A G +I+L+ +E+ +L + + ++SK I + V Sbjct: 118 APQPAQLTVGGLSLDNATKRADIDGRKIELSLREWTVLEYLMQHASRVVSKQQIIDAVLP 177 Query: 180 MNFDSNTNVVEVAIKRLRAKI-DAPFEQKLLHTVRGMGYVLE 220 D N VEV + R+R KI D+ + T+RG GY+LE Sbjct: 178 WGEDFTINAVEVYVSRIRLKIADSGVA---IRTIRGFGYMLE 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 667,044 Number of extensions: 26208 Number of successful extensions: 232 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 41 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)