BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0113.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 943 0.0 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 196 4e-51 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 194 1e-50 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 102 1e-22 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 98 2e-21 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 943 bits (2437), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/467 (99%), Positives = 466/467 (99%) Query: 1 VRLRWLPLRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISY 60 +RLRWLPLRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISY Sbjct: 1 MRLRWLPLRQSITARLVWMSAASALLIFALTGMVLYTVLKGELLRHERDGLVTTLNDISY 60 Query: 61 QIGRAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLAPQAIRADEGNSV 120 QIGRAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLAPQAIRADEGNSV Sbjct: 61 QIGRAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLAPQAIRADEGNSV 120 Query: 121 GRLHLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVL 180 GRLHLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVL Sbjct: 121 GRLHLPGRRYPLHIMTRTLPAFEERPAVDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVL 180 Query: 181 AVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIED 240 AVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIED Sbjct: 181 AVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIED 240 Query: 241 AYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVND 300 AYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVND Sbjct: 241 AYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVND 300 Query: 301 MLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSDMRIEVAGDTEATAMMETALF 360 MLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDS MRIEVAGDTEATAMMETALF Sbjct: 301 MLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMMETALF 360 Query: 361 KRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDS 420 KRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDS Sbjct: 361 KRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDS 420 Query: 421 ARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIARQ 467 ARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIARQ Sbjct: 421 ARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEGVNSIGFTIARQ 467 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 196 bits (497), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 109/281 (38%), Positives = 177/281 (62%), Gaps = 4/281 (1%) Query: 176 LGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGAL 235 +G +LA++ SY++ R+GL PLR M+ + ++ S +L R+ ++A P+EL + +FN L Sbjct: 176 VGALLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEML 235 Query: 236 DRIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIR 295 DR+ ++ QL F+AD+AH++RTPL NL+ +TQVAL + R E++ +L SN+EE +R+ Sbjct: 236 DRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLS 295 Query: 296 SIVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSDMRIEVAGDTEATAMM 355 ++ MLFLAR++ VA E+ + D+FE + D++ +R+ V G A Sbjct: 296 RMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQA-- 353 Query: 356 ETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRF 415 + L +RA+ NL+ NAI+++P ++R+ + + + ++VSNPG I L RLFDRF Sbjct: 354 DATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRF 413 Query: 416 YRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEG 456 YR D +R GS GLGLAIV+++ +HGG+A +S G Sbjct: 414 YRADPSRSSAGGS--SGLGLAIVRSIMMLHGGQASVTSSPG 452 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 194 bits (494), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 107/257 (41%), Positives = 158/257 (61%), Gaps = 8/257 (3%) Query: 195 PLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYTQLEAFNADVAH 254 PL MS RA +P LSAR+ E MP EL +AL+FN LDR+E Y L F+ D+AH Sbjct: 201 PLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLAH 260 Query: 255 ELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAAT 314 E+RTP+ L+G++QVAL + RS E++ VL+SN+EEL R+ IV ++LFLA++D A Sbjct: 261 EIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQADHAALAV 320 Query: 315 ARVPARVADEVQKTVDFFEFVLDDSDMRIEV--AGDTEATAMMETALFKRALTNLIQNAI 372 R +A+E+ + D+FE + D+ MR +V AG +A +M ++R ++NL+ NA+ Sbjct: 321 ERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIM----WRRVVSNLVINAV 376 Query: 373 QHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQDINGSHGHG 432 ++ + +RI E R+ V N + + + R+FDRFYR D +R S +G Sbjct: 377 RYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDRSRSAFTES--NG 434 Query: 433 LGLAIVKAVARMHGGRA 449 LGLAIVKA+ +HGG A Sbjct: 435 LGLAIVKAIMALHGGTA 451 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 102 bits (253), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 92/281 (32%), Positives = 139/281 (49%), Gaps = 40/281 (14%) Query: 187 WIARA-GLAPLRQMSDRAQSFSPRKLSARMPVE--AMPQELSVLALAFNGALDRIEDAYT 243 W+A + GLAP+R+ A + R +++ P+ MPQE+ L A N L R++ A Sbjct: 158 WVAVSRGLAPVRKA---AAEVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHATE 214 Query: 244 QLEAFNADVAHELRTPLANLIGETQV---ALARERSAGEFKDVLQSNLEELDRIRSIVND 300 AF AD AHELRTPL L +TQ+ A+ E F D L++ LE R +V+ Sbjct: 215 TQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFAD-LKNGLE---RTTHLVHQ 270 Query: 301 MLFLARSDQGEAATARVPAR-----VADEVQKTVDFFEFVLDDSDM-----RIEVAGDTE 350 +L LAR + + +V R V ++ + L SD + V G+ + Sbjct: 271 LLTLARQEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNAD 330 Query: 351 ATAMMETALFKRALTNLIQNAIQHSPEGALLRIDIACQEET---VRVAVSNPGKPIDEHH 407 A ++ NL+ NA++++P G + +D+ T V V + G I E Sbjct: 331 ALRIL--------FNNLVDNALRYTPAGGV--VDVGVDLSTAGYCTVVVQDSGPGIAESE 380 Query: 408 LPRLFDRFYRVDSARQDINGSH---GHGLGLAIVKAVARMH 445 LPR+FDRFYR + Q+ +GS G GLGLAIV+ +A +H Sbjct: 381 LPRVFDRFYRAQHS-QEGSGSRAAFGSGLGLAIVRQIADLH 420 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 97.8 bits (242), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 108/413 (26%), Positives = 179/413 (43%), Gaps = 37/413 (8%) Query: 49 DGLVTTLNDISYQIGRAGNTERWGRVQAKLATLSQADQHVSFWISSSDARFTYGDLSRLA 108 DG VT D+ Y + T+ GR+ K+ L+ + VS Y DL + Sbjct: 65 DGHVTA--DVPYVALDSFETDNLGRIYYKVTGLN--GELVS----------GYDDLPPVP 110 Query: 109 PQAIRADEGNSVGRLHLPGRR-YPLHIMTRTLPAFEE--RPAVDVTVGVDGEPYFEMRRT 165 P R++ ++ R + P+ I P +++ R + VG E M R Sbjct: 111 PDVKRSEVYPALVRFYQADYHDQPVRIAALLQPVYDDSIRGIALIQVGETMEGRNGMTRK 170 Query: 166 FLLATSVFSLLGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELS 225 L T + +A + ++ R L PL Q+ +S +P LS + +E+ Sbjct: 171 ILFDTLWRQGTLVTVAALLVWFAVRFVLRPLMQLKGEVESRAPTDLSG-FDTALVHKEMR 229 Query: 226 VLALAFNGALDRIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARER----SAGEFK 281 L A NG + R++ + F AD +H+LRTPL L ++++ L R A E + Sbjct: 230 PLVQAMNGYMGRLQALISAQRRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELR 289 Query: 282 DVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSDM 341 D+++ D + N +L LAR++ G A +P ++D ++ E Sbjct: 290 DLVEGMARTTDAAVHLANRLLTLARAEHGAAEGGTMPLSLSDAARQVA--LELSQQAVSR 347 Query: 342 RIEVAGDTEATAMMETA--LFKRALTNLIQNAIQHSPEGALLRIDIACQEET-------V 392 I +A + E +E L + NL+ NAI+++P G + + + Q + Sbjct: 348 EIGLAFEEEGDVSIEANALLLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALL 407 Query: 393 RVAVSNPGKPIDEHHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMH 445 V S PG P E ++F+ FYR A Q +N G GLGL+IV+ +A MH Sbjct: 408 EVEDSGPGIPAAERE--KVFEPFYRA-GATQHVNPG-GTGLGLSIVREIAAMH 456 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,422,561 Number of extensions: 59891 Number of successful extensions: 357 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 33 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)