BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0112.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid... 627 0.0 gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogen... 180 1e-46 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 81 1e-16 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 80 3e-16 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 75 1e-14 >gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 627 bits (1617), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/319 (98%), Positives = 314/319 (98%) Query: 1 MSALTPISPIAFVSQAGASEVQQWISALQAAMPEERVLPCEQLDADQLAQCRVAIVANPD 60 MSALTPISPIAFVSQAGASEVQQWISALQAAMPEER+LPCEQLDADQLAQCRVAIVANPD Sbjct: 1 MSALTPISPIAFVSQAGASEVQQWISALQAAMPEERILPCEQLDADQLAQCRVAIVANPD 60 Query: 61 PAQLKRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRD 120 PAQLKRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRD Sbjct: 61 PAQLKRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRD 120 Query: 121 MPLYARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAGAGLRQERFAVNGWSRSAREVE 180 MPLYARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAA A LRQ RFAVNGWSRSARE E Sbjct: 121 MPLYARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAAAALRQARFAVNGWSRSAREAE 180 Query: 181 GVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDD 240 GVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDD Sbjct: 181 GVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDD 240 Query: 241 AALKAALDAGQLRHAVLDVFAVEPLPPEAWPWSHPAVTVLPHCSAPTDRQTASAIVAANI 300 AALKAALDAGQLRHAVLDVFAVEPLPP AWPWSHPAVTVLPHCSAPTDRQTASAIVAANI Sbjct: 241 AALKAALDAGQLRHAVLDVFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVAANI 300 Query: 301 RRYRHSGEIAANVDLRRGY 319 RRYRHSGEIAANVDLRRGY Sbjct: 301 RRYRHSGEIAANVDLRRGY 319 >gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 108/255 (42%), Positives = 143/255 (56%) Query: 65 KRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRDMPLY 124 +R P L + SV AG+++ L + R+ +P +AA M + V L LHRD +Y Sbjct: 54 ERFPKLEILFSVGAGIDQFDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVY 113 Query: 125 ARRQREHHWEAQPYVLPEQRRVSLLGLGALGEAAGAGLRQERFAVNGWSRSAREVEGVQT 184 AR+QR+ W A RV +LGLG LG A L F GWSRSA +++G+ Sbjct: 114 ARQQRDQVWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDC 173 Query: 185 FHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAALK 244 + G AGL LA+TD+LVCL+PLT ETRGL+ A LP A LIN RG + + L Sbjct: 174 YSGSAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLL 233 Query: 245 AALDAGQLRHAVLDVFAVEPLPPEAWPWSHPAVTVLPHCSAPTDRQTASAIVAANIRRYR 304 AAL+ GQL AVLDV VEPLPP W+HP + + PH ++ T A V N+RRYR Sbjct: 234 AALNDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRYR 293 Query: 305 HSGEIAANVDLRRGY 319 + +D +RGY Sbjct: 294 EGLPLLGLIDRQRGY 308 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 68/229 (29%), Positives = 113/229 (49%), Gaps = 14/229 (6%) Query: 63 QLKRMPNLLWVHSVWAGVERLVADLGQSDLQLTRLVDPRLAAT----MAEAVLAWTLYLH 118 +++ +P+L V ++ AG E + D+ ++ + A T +A+ L L + Sbjct: 60 EMRALPHLQLVCTLGAGFENV--DVAHAEAHGIEIATG--AGTNEDCVADHALGLLLAIL 115 Query: 119 RDMPLYARRQREHHWEAQPYVLPE--QRRVSLLGLGALGEAAGAGLRQERFAVNGWSRSA 176 R++P+ R R+ W + P+ +RV ++G+G +G+ R F + Sbjct: 116 RNIPVLDRYTRDGGWRETIPLQPQLAGKRVGIVGMGNIGKKIAR--RAAAFDAEIAYCNR 173 Query: 177 REVEGVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGP 236 ++ + V + P +AQ+ D L+ P A+TR LI+A L L PQ L+N RG Sbjct: 174 KKRDDVDYHYFP-DVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGS 232 Query: 237 IIDDAALKAALDAGQLRHAVLDVFAVEPLPPEAWPWSHPAVTVLPHCSA 285 I+D AL AAL +G+L A LDV+ EP PP A + P V + PH + Sbjct: 233 IVDTDALGAALSSGRLAGAGLDVYEGEPQPPAAL-IALPNVVLTPHIAG 280 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 56/143 (39%), Positives = 77/143 (53%), Gaps = 7/143 (4%) Query: 126 RRQREHHWEAQPYVLPE--QRRVSLLGLGALGEAAGAGLRQERFAVNGWSRSAREV-EGV 182 +R RE HW+ + E R + L+GLGA+G A V A+E +GV Sbjct: 127 QRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHDPYAKEAPQGV 186 Query: 183 QTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAA 242 Q L + AQ+D++ PLTAE + +I+A LA + A L+N ARG +ID+ A Sbjct: 187 QLVD----LDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQA 242 Query: 243 LKAALDAGQLRHAVLDVFAVEPL 265 L AALD+G+LR A LD F EP Sbjct: 243 LIAALDSGKLRAAGLDSFEKEPF 265 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 64/207 (30%), Positives = 102/207 (49%), Gaps = 11/207 (5%) Query: 106 MAEAVLAWTLYLHRDMPLYARRQREHHWEAQPYVLPEQ---RRVSLLGLGALGEAAGAGL 162 +A+ +A L R++ R R W+ P+ + R+ ++GLG +G+A Sbjct: 107 VADLAMALMLATARNVVRADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRA 166 Query: 163 RQERFAVNGWSRSAREVEGVQTFHGPAGLAQMLAQTDILVCLLPLTAETRGLIDAAHLAM 222 ++ +RS ++V + + + + D LV + P A TR L++A L Sbjct: 167 AAFDMQISYHNRSRKDVP----YTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEA 222 Query: 223 LPPQAQLINFARGPIIDDAALKAALDAGQLRHAVLDVFAVEP-LPPEAWPWSHPAVTVLP 281 L P+ LIN ARG ++D+AAL AAL G + A LDVFA EP +P E + V + P Sbjct: 223 LGPKGFLINVARGSVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALDN--VVLTP 280 Query: 282 HCSAPT-DRQTASAIVAANIRRYRHSG 307 H ++ T +TA A +A N + SG Sbjct: 281 HMASGTLVTRTAMADLAFNNLQAHFSG 307 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.133 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 972,327 Number of extensions: 37169 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 9 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)