BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0109.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 833 0.0 gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 247 1e-66 gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 69 6e-13 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 833 bits (2152), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/416 (98%), Positives = 413/416 (99%) Query: 1 MRATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSASQPGAGAPMP 60 MRATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSA+QPGAGAPMP Sbjct: 1 MRATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMP 60 Query: 61 GLELARRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPVTA 120 GLELARRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDP TA Sbjct: 61 GLELARRVEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTA 120 Query: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY Sbjct: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 Query: 181 LSFPNWRSWRKSFAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPL 240 LSFPNWRSWRKS AFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPL Sbjct: 181 LSFPNWRSWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPL 240 Query: 241 LQTLSTLTPSPFDTRTPTAPEKVAEPRISREQAVALGQAQARKMGLTEPVGGVFYSGMFG 300 LQTLSTLTPSPFDTRTPTAPEKVAEPRI+REQAVALGQAQARKMGLTEPVGGVFYSGMFG Sbjct: 241 LQTLSTLTPSPFDTRTPTAPEKVAEPRITREQAVALGQAQARKMGLTEPVGGVFYSGMFG 300 Query: 301 VYGVGYFQPGNDHGDVGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 VYGVGYFQPGNDHGD+GLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL Sbjct: 301 VYGVGYFQPGNDHGDIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHSGRIL 360 Query: 361 GLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERTVAQKTPAREGPVAELQ 416 GLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERT AQKTPAREGPVAELQ Sbjct: 361 GLPGRILISFMGALVAMLSVTGVIIWVRKRKARLQQRERTAAQKTPAREGPVAELQ 416 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 247 bits (631), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 159/427 (37%), Positives = 233/427 (54%), Gaps = 25/427 (5%) Query: 2 RATLVLLHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSASQPGAGAPMPG 61 R LVLLHRW GL A FL + LTG+++ +D ELDAWL PQL A+ A A A M Sbjct: 19 RHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDP 78 Query: 62 LELARRVEQAE-PRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPVTA 120 L +V QA P++ ++ ++ A PG T+ +P DPATGK Y L Y++I V+P T Sbjct: 79 YLLREKVAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRLGYDEILVNPYTG 138 Query: 121 EIQGRRMWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALY 180 +QG R ISL +L+PFL+KLH+++ LP G LL+G +G++W LDC + + Sbjct: 139 VVQGVRDRDKISLKPADLMPFLFKLHHSLALPR----APGALLLGTVGLLWTLDCFVGMI 194 Query: 181 LSFPNWR----SWRKSFAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQV 236 L++P R W+ ++ +WR G +++N DLHR+ G+W W +LL+ A +SV +NL Q+ Sbjct: 195 LTWPRARPFLVKWKPAWLIKWRAGFYRVNLDLHRAFGLWCWVMLLLFAWSSVMLNLRDQI 254 Query: 237 MRPLLQTLSTLTPSPFDTR---TPTAPEKVAEPRISREQAVALGQAQ----ARKMGLT-E 288 P++ + + FDT P P+ ++ P S +A AL + AR+ GL + Sbjct: 255 YLPVMSRILS-----FDTSWRPGPPLPQALSGPPQSWPEAHALARRTMDGFARQRGLVID 309 Query: 289 PVGGVFYSGMFGVYGVGYFQPGNDHGDVGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFL 348 + + GVY Y N +GN L DA +G G +P G+ F Sbjct: 310 FEEKLDFDRRRGVY--IYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFS 367 Query: 349 QAQFPLHSGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKA-RLQQRERTVAQKTPA 407 LH G + GLP RIL+ +G V++L+VTGV++W +KR A RLQ R +K A Sbjct: 368 NWLGALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWWKKRAARRLQLRSPGDGRKPSA 427 Query: 408 REGPVAE 414 VA Sbjct: 428 SHSDVAS 434 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 69.3 bits (168), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 94/398 (23%), Positives = 167/398 (41%), Gaps = 49/398 (12%) Query: 8 LHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSASQPGAGAPMPGLELARR 67 LH + G+ A L G+TGA++++ E+ L+P + + Q A + ++ + Sbjct: 9 LHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQ----ATLTPQQVLEK 64 Query: 68 VEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLD-YNQIAVDPVTAEIQGRR 126 + Q EP+ R+T + AEPG ++ ++ P G+ +D Y + PVT Sbjct: 65 LRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTG------ 118 Query: 127 MWGAISLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALYLSFP-- 184 F + H + LP E G +++G + + + L LYL +P Sbjct: 119 ---------MEFFEFTERFHRWLLLPT----EIGKIVLGTLALCLLMLALSGLYLRWPRR 165 Query: 185 --NWRSW-RKSFAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPLL 241 + R+W R F + G L ++LH G W + +I A T L+ + + Sbjct: 166 ALSLRAWLRLDFQLQ----GRSLLWNLHSVIGTWSLVMYVIFATTGAYWALD--WFKDGV 219 Query: 242 QTLSTLTPSPFDTRTPTAP-EKVAEPRISREQAVALGQAQARKMGLTEPVGGVFYSGMF- 299 TL+ T R P A E + + + VAL QA LT+ + S Sbjct: 220 NTLAGET-----RRAPMARGEGKKKDGPNNQTPVALDLTQAWNAFLTKAPD--YQSAQLR 272 Query: 300 ----GVYGVGYFQPGNDHGDVGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLH 355 G + F G+D + + SG + + S G F+ A +PLH Sbjct: 273 LPEQGTQPLQIFFLGHD-APHERARSRMVVLPQSGEIKQLERYEDKSTGGRFIAAIYPLH 331 Query: 356 SGRILGLPGRILISFMGALVAMLSVTGVIIWVRKRKAR 393 G GLPGRI+++ ++ + +TG ++++ +R+ + Sbjct: 332 MGTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKK 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,397,750 Number of extensions: 62817 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 3 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)