BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0108.p (301 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 576 e-165 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 94 2e-20 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 88 8e-19 gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 82 6e-17 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 576 bits (1484), Expect = e-165, Method: Compositional matrix adjust. Identities = 300/301 (99%), Positives = 300/301 (99%) Query: 1 VVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTEL 60 VVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTEL Sbjct: 978 VVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTEL 1037 Query: 61 AQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQRAHR 120 AQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQRAHR Sbjct: 1038 AQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQRAHR 1097 Query: 121 VELAQVTRERDQAAASATIHVLPRTEREPVAQADPLVREAHGAGGASAAQPLPAYPRPEP 180 VELAQVTRERDQAAASATIHVLPRTEREPVAQADPLVREAHGAGGASAAQPLP YPRPEP Sbjct: 1098 VELAQVTRERDQAAASATIHVLPRTEREPVAQADPLVREAHGAGGASAAQPLPDYPRPEP 1157 Query: 181 QEDAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNL 240 QEDAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNL Sbjct: 1158 QEDAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNL 1217 Query: 241 PGMSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAHALPKPYDIDQLQEILDKIAA 300 PGMSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAHALPKPYDIDQLQEILDKIAA Sbjct: 1218 PGMSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAHALPKPYDIDQLQEILDKIAA 1277 Query: 301 G 301 G Sbjct: 1278 G 1278 Score = 57.8 bits (138), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 42/108 (38%), Positives = 62/108 (57%), Gaps = 5/108 (4%) Query: 189 LLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQ-GFDVLFTDVNLPGMSGV- 246 +LVVED+ + Q V + L +LG++ +GE AL+ L+ D++FTDV +PG V Sbjct: 976 ILVVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVT 1035 Query: 247 ELAKAALGRFPDLAVIFASGY--GTMVAR-NVGFAAHALPKPYDIDQL 291 ELA+ A PD+ ++F SGY T+V + L KPY I+QL Sbjct: 1036 ELAQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQL 1083 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 45/117 (38%), Positives = 76/117 (64%) Query: 1 VVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTEL 60 VV+D+ ++++ V + LS GY VL+A++G AL ILQS IDL+ TDV +PG + ++ Sbjct: 725 VVDDEASIRMLVAEILSDAGYDVLQAEDGPGALRILQSKQRIDLLITDVGLPGGLNGRQV 784 Query: 61 AQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQR 117 A R PD+ +LF +GY + G L+ G++L++KP+++ LA+R++ +L R Sbjct: 785 ADAGRAARPDLHVLFITGYAENAAFDNGHLERGMQLMTKPFKMEALARRVQSILESR 841 Score = 54.7 bits (130), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 40/121 (33%), Positives = 61/121 (50%), Gaps = 5/121 (4%) Query: 183 DAGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQALE-EQGFDVLFTDVNLP 241 D G +LVV+D + LV E+L G+ G AL+ L+ +Q D+L TDV LP Sbjct: 717 DGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAEDGPGALRILQSKQRIDLLITDVGLP 776 Query: 242 -GMSGVELAKAALGRFPDLAVIFASGYGTMVARNVGF---AAHALPKPYDIDQLQEILDK 297 G++G ++A A PDL V+F +GY A + G + KP+ ++ L + Sbjct: 777 GGLNGRQVADAGRAARPDLHVLFITGYAENAAFDNGHLERGMQLMTKPFKMEALARRVQS 836 Query: 298 I 298 I Sbjct: 837 I 837 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 39/116 (33%), Positives = 78/116 (67%) Query: 1 VVEDDPNLQLTVIDTLSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTEL 60 VV+D+P +++ ++ L LGY VL+A++G AL ++++ IDL+ TDV +P + +L Sbjct: 567 VVDDEPLVRMVTVEVLRDLGYSVLEAEDGPSALRVVEACQEIDLLLTDVGLPNGMNGRQL 626 Query: 61 AQQARLLVPDIEILFTSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQMLSQ 116 A R PD+ +LF +GY + +++ G L+ G+++L+KP+ + LA+R+++++ + Sbjct: 627 ADAIRAPRPDLPVLFVTGYAENAVLNHGHLERGMQVLTKPFAADVLARRVKELVGE 682 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 82.0 bits (201), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 41/99 (41%), Positives = 69/99 (69%), Gaps = 1/99 (1%) Query: 16 LSSLGYRVLKADNGERALEILQSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILF 75 L SLGYRV ++ AL++++SG +DL+FTD++MPGS+ LA++A+ ++P I++L Sbjct: 448 LESLGYRVSMVNSPAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLPTIKVLL 507 Query: 76 TSGYPRDTIVHEGRLDAGVELLSKPYRINQLAQRIRQML 114 T+GY +++ G D +L KPYR ++LA++IR +L Sbjct: 508 TTGYASESLERHG-ADGEFPVLDKPYRHDELARKIRIVL 545 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 51/156 (32%), Positives = 74/156 (47%), Gaps = 14/156 (8%) Query: 150 VAQADPLVREAHGA-GGASAAQPLPAYPR---------PEPQEDAGGLRLLVVEDNIDSQ 199 V Q+ VR GA GG + PA Q G +LVV+D ++ Sbjct: 382 VKQSGGSVRITSGAEGGTTVTIYFPATQDQVGGGFEYDNRSQSQGGQEHILVVDDRLEVA 441 Query: 200 QLVCELLETLGHQARGVISGEEALQALEE-QGFDVLFTDVNLPG-MSGVELAKAALGRFP 257 +L +LE+LG++ V S EALQ + + D+LFTD+ +PG M+GV LA+ A P Sbjct: 442 ELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRMLP 501 Query: 258 DLAVIFASGYGTMVARNVGFAAH--ALPKPYDIDQL 291 + V+ +GY + G L KPY D+L Sbjct: 502 TIKVLLTTGYASESLERHGADGEFPVLDKPYRHDEL 537 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 985,047 Number of extensions: 43450 Number of successful extensions: 313 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 9 Length of query: 301 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 216 Effective length of database: 1,217,395 Effective search space: 262957320 Effective search space used: 262957320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)