BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0104.p (732 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311807|ref|YP_003775899.1| light-regulated signal transduc... 1492 0.0 gi|300312674|ref|YP_003776766.1| two-component system transcript... 128 2e-30 gi|300312803|ref|YP_003776895.1| two component sensor histidine ... 104 2e-23 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 84 6e-17 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 76 9e-15 >gi|300311807|ref|YP_003775899.1| light-regulated signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 732 Score = 1492 bits (3862), Expect = 0.0, Method: Compositional matrix adjust. Identities = 732/732 (100%), Positives = 732/732 (100%) Query: 1 MSNPYHEGRHRTVDLSNCDSEPIHIPGSIQNHGALLCFAPDGALASRSANASTLLGALPE 60 MSNPYHEGRHRTVDLSNCDSEPIHIPGSIQNHGALLCFAPDGALASRSANASTLLGALPE Sbjct: 1 MSNPYHEGRHRTVDLSNCDSEPIHIPGSIQNHGALLCFAPDGALASRSANASTLLGALPE 60 Query: 61 VGQALGEAHLDASARAVIQAALEDRYGYVDARMLVLPAGVFDLVTHKSEGVLIAEFERRP 120 VGQALGEAHLDASARAVIQAALEDRYGYVDARMLVLPAGVFDLVTHKSEGVLIAEFERRP Sbjct: 61 VGQALGEAHLDASARAVIQAALEDRYGYVDARMLVLPAGVFDLVTHKSEGVLIAEFERRP 120 Query: 121 ADVAALDSFALAAHQAIQRIQRQQSVADLLSLAVDEIARITGFDRVMAYRFLHDDSGEVV 180 ADVAALDSFALAAHQAIQRIQRQQSVADLLSLAVDEIARITGFDRVMAYRFLHDDSGEVV Sbjct: 121 ADVAALDSFALAAHQAIQRIQRQQSVADLLSLAVDEIARITGFDRVMAYRFLHDDSGEVV 180 Query: 181 AEHRREDLESFLGQRYPASDIPAQARRLYVINPIRLIADVGAPTVPLQPDLNPLTGRALD 240 AEHRREDLESFLGQRYPASDIPAQARRLYVINPIRLIADVGAPTVPLQPDLNPLTGRALD Sbjct: 181 AEHRREDLESFLGQRYPASDIPAQARRLYVINPIRLIADVGAPTVPLQPDLNPLTGRALD 240 Query: 241 LSHGILRSVSPVHIEYLSNMGVGASMSLSIVIGERLWGLIACHHMSPHLVPHAVRMSCQL 300 LSHGILRSVSPVHIEYLSNMGVGASMSLSIVIGERLWGLIACHHMSPHLVPHAVRMSCQL Sbjct: 241 LSHGILRSVSPVHIEYLSNMGVGASMSLSIVIGERLWGLIACHHMSPHLVPHAVRMSCQL 300 Query: 301 LSQFVSALVERNLNGERARALDDSTALRREIVAQVTDREDLVLALSSAPESFLRLMNAHG 360 LSQFVSALVERNLNGERARALDDSTALRREIVAQVTDREDLVLALSSAPESFLRLMNAHG Sbjct: 301 LSQFVSALVERNLNGERARALDDSTALRREIVAQVTDREDLVLALSSAPESFLRLMNAHG 360 Query: 361 GAITLDRKTQVFGKAPSREGVNALINWLNEHHSADLFSTSSLGADVPDLKAAMGDICGVL 420 GAITLDRKTQVFGKAPSREGVNALINWLNEHHSADLFSTSSLGADVPDLKAAMGDICGVL Sbjct: 361 GAITLDRKTQVFGKAPSREGVNALINWLNEHHSADLFSTSSLGADVPDLKAAMGDICGVL 420 Query: 421 AVRFYREQNGYAFWFRNEQVEDVRWGGNPEKKYSIGPLGPRLTPRGSFAVWKETVRGKSL 480 AVRFYREQNGYAFWFRNEQVEDVRWGGNPEKKYSIGPLGPRLTPRGSFAVWKETVRGKSL Sbjct: 421 AVRFYREQNGYAFWFRNEQVEDVRWGGNPEKKYSIGPLGPRLTPRGSFAVWKETVRGKSL 480 Query: 481 PWEKTQTEAASQLRLDLQEVALTNENLVKRARETLLATLGHDLRDPLQAIMMAARMIEVR 540 PWEKTQTEAASQLRLDLQEVALTNENLVKRARETLLATLGHDLRDPLQAIMMAARMIEVR Sbjct: 481 PWEKTQTEAASQLRLDLQEVALTNENLVKRARETLLATLGHDLRDPLQAIMMAARMIEVR 540 Query: 541 EHSPSSSNLSKRISSSSSRMHRLISQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEAR 600 EHSPSSSNLSKRISSSSSRMHRLISQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEAR Sbjct: 541 EHSPSSSNLSKRISSSSSRMHRLISQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEAR 600 Query: 601 MAYPDNEIILEAEDCGEIALDGDRISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIA 660 MAYPDNEIILEAEDCGEIALDGDRISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIA Sbjct: 601 MAYPDNEIILEAEDCGEIALDGDRISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIA 660 Query: 661 VSNHGPAIPEPVRSNLFKPYKKESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAVSCDNNI 720 VSNHGPAIPEPVRSNLFKPYKKESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAVSCDNNI Sbjct: 661 VSNHGPAIPEPVRSNLFKPYKKESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAVSCDNNI 720 Query: 721 ITFTVTLPILAD 732 ITFTVTLPILAD Sbjct: 721 ITFTVTLPILAD 732 >gi|300312674|ref|YP_003776766.1| two-component system transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 128 bits (321), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 87/245 (35%), Positives = 134/245 (54%), Gaps = 19/245 (7%) Query: 491 SQLRLDLQEVALTNENLVKRARETLLATLGHDLRDPLQAIMMAARMIEVREHSPSSSNLS 550 QL L+L+E E L R E +A LGHDLR+PL A+MMAA +++ + S Sbjct: 138 QQLALELRE---RTETL--RLNEMFVAILGHDLRNPLSAVMMAATLLQRTYKEEVAQKAS 192 Query: 551 KRISSSSSRMHRLISQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEI-- 608 ++I + RM +LI +LD++R + G+ + R + ++ +V E + YP + I Sbjct: 193 QQILNGGKRMAKLIENMLDLARARLAGGIPLARASACLGEITERVVQEYQATYPGSVIER 252 Query: 609 ILEAEDCGEIALDGDRISQVVSNLISNTRHHGEIGKPSTL-IVFRREDVLTIAVSNHGPA 667 +E +D G DGDR++QV +NL+ N HGE G P + I R E + + ++N G Sbjct: 253 RIEGDDNG--YWDGDRLAQVAANLLGNALQHGEPGHPICIGIDGRSEHEVVMVIANRG-V 309 Query: 668 IPEPVRSNLFKPYKKESLGNQRNT---RGLGLGLYIVSEIVHGHGGKIAVSCDNNIITF- 723 IP + +F P++ G +R GLGLGL+I +IV HGG I++S D+ + T Sbjct: 310 IPADLLPVIFDPFR----GGEREAGRKEGLGLGLFIAQQIVKAHGGSISMSSDDQLQTMV 365 Query: 724 TVTLP 728 TV LP Sbjct: 366 TVRLP 370 >gi|300312803|ref|YP_003776895.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 104 bits (260), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 72/230 (31%), Positives = 116/230 (50%), Gaps = 9/230 (3%) Query: 506 NLVKRARETLLATLGHDLRDPLQAIMMAARMIEVREH-SPSSSNLSKRISSSSSRMHRLI 564 L+ ++R LA LGHDLR+PL A + A+++ + E ++ RI +S RM +L+ Sbjct: 151 KLLDQSRNLFLAILGHDLRNPLGATIHGAQIMLLAEDIDDRHRKIAARIYNSGQRMSKLV 210 Query: 565 SQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEIILEAEDCGEI--ALDG 622 + +LD +R G GL ++ +V + ++ E ++PD I L+ GE+ D Sbjct: 211 ADLLDYTRTHLGSGLPLEISSANVGDIAQRVIDEISTSHPDKVITLKT--SGELNGEWDK 268 Query: 623 DRISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIAVSNHGPAIPEPVRSNLFKPYKK 682 +RI+Q +SNL+ N HG G+ + + + +A++N GP IPE +F P + Sbjct: 269 ERIAQAISNLLGNAVQHGATGEAIKVEISASRGEVHVAINNKGPLIPEEKLHTIFDPLVR 328 Query: 683 ---ESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAVSCDN-NIITFTVTLP 728 +GLGLYIV E+ HGG + S TFT+ LP Sbjct: 329 LVANGGEGSGQGGSMGLGLYIVREVASAHGGSVTASSSTAEGTTFTIRLP 378 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 83.6 bits (205), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 78/249 (31%), Positives = 130/249 (52%), Gaps = 21/249 (8%) Query: 495 LDLQEVALTNENLVKRARETLLATLGHDLRDPLQAIMMAARMIEVREHSPSSSNLSK--R 552 L+ Q A + V R ++ LATL H+LR PL AI+ + + + +HS + L K + Sbjct: 33 LETQRAARRQADSVTRLKDEFLATLSHELRTPLNAIVGWTQYL-LNDHS-DADRLVKGLQ 90 Query: 553 ISSSSSRMH-RLISQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEIILE 611 + ++R+ +++ +LD+SR+ +G L ++ RP+D+ + E ++ R A +I L Sbjct: 91 VIERNARLQAQMVEDLLDMSRMLAGK-LRLETRPMDLASVAEEAIAAVRPAAEARQIELH 149 Query: 612 AE--DCGEIALDGDRISQVVSNLISN----TRHHGEIGKPSTLIVFRREDVLTIAVSNHG 665 G I D R+ QVV NL+ N T G + +L + + +D + + VS++G Sbjct: 150 PALGQGGVILGDPARLQQVVWNLLMNAIKFTPRQGRV----SLSLRQHDDTVALEVSDNG 205 Query: 666 PAIPEPVRSNLFKPYKKE--SLGNQRNT-RGLGLGLYIVSEIVHGHGGKI-AVSCDNNI- 720 I ++F + +E S G+ +T GLGLGL I +V HGG+I A S + Sbjct: 206 RGIKGEFLGSVFDRFTQEDVSTGSTGSTASGLGLGLAIARHLVELHGGQIRAASAGPGMG 265 Query: 721 ITFTVTLPI 729 FTVTLP+ Sbjct: 266 AAFTVTLPL 274 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 76.3 bits (186), Expect = 9e-15, Method: Compositional matrix adjust. Identities = 62/229 (27%), Positives = 104/229 (45%), Gaps = 10/229 (4%) Query: 506 NLVKRARETLLATLGHDLRDPLQAIMMAARMI-EVREHSPSSSNLSKRISSSSSRMHRLI 564 N ++A++ +A + H LR PL + + ++ + + P ++ ++SS RM R Sbjct: 231 NKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQS 290 Query: 565 SQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEIILEAEDCGEIALDGDR 624 Q+L ++R + G+ + + + K+V E V + D + I D L GDR Sbjct: 291 RQLLALARSEPGLFESRKLEELSLDKLVEESVQHF-IEEADKKRIDLGFDLRPARLRGDR 349 Query: 625 --ISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIAVSNHGPAIPEPVRSNLFKPYKK 682 +S ++ NLI N + T+ +D ++V + GP I R +F + + Sbjct: 350 FLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYR 409 Query: 683 ESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAVSC--DNNIITFTVTLPI 729 + G GLGL IV EI H HGG I+V C FTV P+ Sbjct: 410 AN----DKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPL 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,188,549 Number of extensions: 91475 Number of successful extensions: 362 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 339 Number of HSP's successfully gapped: 31 Length of query: 732 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 640 Effective length of database: 1,184,250 Effective search space: 757920000 Effective search space used: 757920000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)