BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0101.p (535 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311810|ref|YP_003775902.1| two component sensor histidine ... 1061 0.0 gi|300311887|ref|YP_003775979.1| two component sensor histidine ... 128 1e-30 gi|300313070|ref|YP_003777162.1| two component sensor histidine ... 100 3e-22 gi|300310697|ref|YP_003774789.1| two component hybrid sensor his... 89 6e-19 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 82 1e-16 >gi|300311810|ref|YP_003775902.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 535 Score = 1061 bits (2745), Expect = 0.0, Method: Compositional matrix adjust. Identities = 531/535 (99%), Positives = 534/535 (99%) Query: 1 MRLLTKGLLIVAIPGLFEIVLLAALFKSQADAADAERWAAHSAEVINKAADVREPLLLES 60 MRLLTKGLLIVAIPGLFEIVLLAALFKSQADAADAERWAAHSAEVINKAADVREPLLLES Sbjct: 1 MRLLTKGLLIVAIPGLFEIVLLAALFKSQADAADAERWAAHSAEVINKAADVREPLLLES 60 Query: 61 ARIRNAIIIDDPSPISRPELWTDLEIKLGDLARLVSDNPEQSRSVQALRELVGQFRVWAD 120 ARIRNAIIIDDPSPISRPELWTDLEIKLGDLARLVSDNPEQS SVQALRELVGQFRVWAD Sbjct: 61 ARIRNAIIIDDPSPISRPELWTDLEIKLGDLARLVSDNPEQSHSVQALRELVGQFRVWAD 120 Query: 121 SARERMQRGQRDELVRQLRDEQGPHRLSDFLNRLEGFLNEERHLGSLRNQALQSARQTQT 180 SARERMQRGQRDELVRQLRDEQGPHRLSDFLNRL+GFLNEERHLGSLRNQALQSARQTQT Sbjct: 121 SARERMQRGQRDELVRQLRDEQGPHRLSDFLNRLDGFLNEERHLGSLRNQALQSARQTQT 180 Query: 181 AWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARRLADGLPLAARVKGHDEISLLDD 240 AWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARRLADGLPLAARVKGHDEISLLDD Sbjct: 181 AWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARRLADGLPLAARVKGHDEISLLDD 240 Query: 241 ALHRSSERLQEASQQSEQYRSQLERRARELTEVNADLLQQTQDNEMFIYSVSHDLRSPLV 300 ALHRSSERLQEASQQSEQYRSQLERRARELTE+NADLLQQTQDNEMFIYSVSHDLRSPLV Sbjct: 241 ALHRSSERLQEASQQSEQYRSQLERRARELTELNADLLQQTQDNEMFIYSVSHDLRSPLV 300 Query: 301 NLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIID 360 NLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIID Sbjct: 301 NLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIID 360 Query: 361 SMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAMVEVAPELPPAFGDPTAIEQ 420 SMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGA+VEVAPELPPAFGDPTAIEQ Sbjct: 361 SMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAVVEVAPELPPAFGDPTAIEQ 420 Query: 421 VLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFI 480 VLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFI Sbjct: 421 VLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFI 480 Query: 481 AFQRLHGNAAPGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALPARPPAPV 535 AFQRLHGNAAPGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALPARPPAPV Sbjct: 481 AFQRLHGNAAPGEGIGLALVKRVVERHGGRIWVESTEGQGSSFYVALPARPPAPV 535 >gi|300311887|ref|YP_003775979.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 633 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 111/382 (29%), Positives = 171/382 (44%), Gaps = 50/382 (13%) Query: 159 NEERHLGSLRNQALQSARQTQTAWLIAAVIGSILAAALASVAFTRSIGGRISVLIVNARR 218 E + LG R Q + R T T L A L R I G + +N RR Sbjct: 274 TELKWLGYTREQVV--GRMTHTDLLAPASAERYLRELQPQFMLKREISG----IDLNYRR 327 Query: 219 LADGLPLAARVKG-----HDEISLLDDALHRSSERLQEASQQSEQYRSQLERRARELTEV 273 ADG A V D L+ + + A ++ E + L+R+A L V Sbjct: 328 -ADGSEFTALVNATAIPSRDRSKLISRTVIYDISDRKRAEEEIEALNADLKRQALHLHSV 386 Query: 274 NADLLQQTQDNEMFIYSVSHDLRSPLVNLQGFSKEIGHATTDLLNAVQALPVPDADKAKL 333 N +L E F YSVSHDLR+PL + G++ + D ++ +K + Sbjct: 387 NKEL-------ESFSYSVSHDLRAPLRAISGYAMILEEDYADAID----------EKGR- 428 Query: 334 RSVVADDIQPALRFIQNAVTRSAGIIDSMLRLSRAGRVEYQPVMLDMNVIAQRIVAAMSG 393 L+ I+ V + +I+ +L+L+++ E M+ + ++++A + Sbjct: 429 ---------EQLQVIRRNVRKMDELINDLLKLAKSTTGELTLERFSMDDLVRQVIAGLR- 478 Query: 394 SIRDKGAMVEVAPELPPAFGDPTAIEQVLGNLVGNAVNYLDPARPGRITIDHLREEGGAA 453 R+ ++ P L A + I QV NL+ NAV + + + + E Sbjct: 479 --RENPNVLFDVPPLEGAVANRGLITQVWENLLSNAVKFSSKSEQPAVRVSMQVE----- 531 Query: 454 TGPETNVYVVRDNGLGIPPAYMHKMFIAFQRLH-GNAAPGEGIGLALVKRVVERHGGRIW 512 PE +Y V DNG+G Y HK+F FQRLH G GIGLALV+R+V RH GR+W Sbjct: 532 --PEEYIYGVHDNGVGFDMRYAHKLFGTFQRLHRQEEYAGTGIGLALVQRIVIRHTGRVW 589 Query: 513 VESTEGQGSSFYVALPARPPAP 534 +S G+G+SFY +LP + +P Sbjct: 590 AQSKPGEGASFYFSLPRKGLSP 611 >gi|300313070|ref|YP_003777162.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 939 Score = 100 bits (249), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 91/296 (30%), Positives = 135/296 (45%), Gaps = 37/296 (12%) Query: 249 LQEASQQSEQYRSQLERRAREL--TEVNADLLQQTQDNEM---FIYSVSHDLRSPLVNLQ 303 L E Q E + SQ+ L EV D L + + + ++SHDLR+PL +L Sbjct: 668 LPENRQMLEIFCSQIAMAIERLHYAEVAQDALVTMESERLRNSLLSAISHDLRTPLTSLV 727 Query: 304 GFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRFIQNAVTRSAGIIDSML 363 G + D+L A A+L DD + R I + G+++++L Sbjct: 728 G--------SADVLAA----------NARLD----DDARAMARTISEQSQKMVGLMNNLL 765 Query: 364 RLSR--AGRVEYQPVMLDMNVIAQRIVAAMSGSIRDKGAMVEVAPELPPAFGDPTAIEQV 421 ++R AG V + +A + ++ ++ + +APELP D +E+V Sbjct: 766 DMARLQAGVVTLNRQWNALEELAGSALRLLAKALEGRQVDTRIAPELPLLRVDAVLLERV 825 Query: 422 LGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVYVVRDNGLGIPPAYMHKMFIA 481 L NLV NA+ Y P P I I E G A P+ V V+ D G G PP F Sbjct: 826 LANLVENAIKYSPPGTP--IGISAHVEHGDGA--PQVKVCVM-DQGRGFPPTMTQHAFEK 880 Query: 482 FQRLHG-NAAPGEGIGLALVKRVVERHGGRIWVESTE-GQGSSFYVALPARP-PAP 534 F R +A PG G+GLA+ + ++E H GRIW+ E GQG++ LP PAP Sbjct: 881 FTRGDNESATPGVGLGLAICRAIIEAHQGRIWIAPQEAGQGATVCFTLPVETQPAP 936 >gi|300310697|ref|YP_003774789.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 916 Score = 89.4 bits (220), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 81/310 (26%), Positives = 140/310 (45%), Gaps = 62/310 (20%) Query: 242 LHRSSERLQEASQQSEQYRSQLERRARELTEVNADLLQQTQD--------------NEMF 287 LHR E++Q + L R RE ++ + +TQ+ F Sbjct: 404 LHRKHEQMQW---------TMLYLRVREQLKLEKAVAARTQELRQALQAADEANLAKSGF 454 Query: 288 IYSVSHDLRSPLVNLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADDIQPALRF 347 + +SHDLR+PL ++ GF+ + +QA P A R Sbjct: 455 LTRISHDLRTPLTSILGFA-----------DMIQASGGPHAGHG--------------RI 489 Query: 348 IQNAVTRSAGIIDSMLRLSRAGRVEY-QPVMLDMNVIAQRIVAAMSGSI----RDKGAMV 402 I + +++ ++ +R ++ QP L ++ + Q I A G + + + Sbjct: 490 IMRSARHMLTMVNDLIDYARGDPLDTPQPTPLYLHALLQEI--AQEGQVLAQRQHNHFTL 547 Query: 403 EVAPELPPAFG-DPTAIEQVLGNLVGNAVNYLDPARPGRITIDHLREEGGAATGPETNVY 461 +++P+LPP D + ++LGNL+ NA Y R GRI + H++ + A + + Sbjct: 548 DISPDLPPVLALDARRLHRILGNLLDNAAKY---TREGRIGL-HVQWQADHADAGKGALQ 603 Query: 462 V-VRDNGLGIPPAYMHKMFIAFQRLHGNA-APGEGIGLALVKRVVERHGGRIWVESTEGQ 519 + V D G GI P + ++F F+R + PG G+GLA+V+ V+R GG I +ES G Sbjct: 604 IEVSDTGCGIAPQFQSRIFEPFERADADRRQPGIGMGLAIVRLWVQRMGGSIVLESQPGM 663 Query: 520 GSSFYVALPA 529 G+ ++ LPA Sbjct: 664 GTRMHLQLPA 673 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 82.0 bits (201), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 70/260 (26%), Positives = 128/260 (49%), Gaps = 45/260 (17%) Query: 281 TQDNEMFIYSVSHDLRSPLVNLQGFSKEIGHATTDLLNAVQALPVPDADKAKLRSVVADD 340 T+ + F+ ++SH+LR+PL + G+++ + + +D AD Sbjct: 47 TRLKDEFLATLSHELRTPLNAIVGWTQYLLNDHSD----------------------ADR 84 Query: 341 IQPALRFIQNAVTRSAGIIDSMLRLSR--AGRVEYQPVMLDMNVIAQRIVAAMSGSIRDK 398 + L+ I+ A +++ +L +SR AG++ + +D+ +A+ +AA+ + + Sbjct: 85 LVKGLQVIERNARLQAQMVEDLLDMSRMLAGKLRLETRPMDLASVAEEAIAAVRPAAEAR 144 Query: 399 GAMVEVAPELPPA---FGDPTAIEQVLGNLVGNAVNYLDPARPGRITIDHLREEGGAATG 455 +E+ P L GDP ++QV+ NL+ NA+ + R GR+++ LR+ Sbjct: 145 --QIELHPALGQGGVILGDPARLQQVVWNLLMNAIKF--TPRQGRVSLS-LRQH------ 193 Query: 456 PETNVYVVRDNGLGIPPAYMHKMFIAFQR------LHGNAAPGEGIGLALVKRVVERHGG 509 +T V DNG GI ++ +F F + G+ A G G+GLA+ + +VE HGG Sbjct: 194 DDTVALEVSDNGRGIKGEFLGSVFDRFTQEDVSTGSTGSTASGLGLGLAIARHLVELHGG 253 Query: 510 RIWVEST-EGQGSSFYVALP 528 +I S G G++F V LP Sbjct: 254 QIRAASAGPGMGAAFTVTLP 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,578,979 Number of extensions: 65120 Number of successful extensions: 450 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 425 Number of HSP's successfully gapped: 30 Length of query: 535 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 445 Effective length of database: 1,193,720 Effective search space: 531205400 Effective search space used: 531205400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)